Emerging Separation Techniques in Supercritical Fluid Chromatography

Yamamoto, Kazuhiro; Machida, Koichi; Kotani, Akio; Hakamata, Hideki

doi:10.1248/cpb.c21-00306

Cited by 5 publications

(3 citation statements)

References 75 publications

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“…SFC is a developing chromatographic technique that is expected to provide faster separation with higher resolutions in comparison with HPLC and GC [1][2][3][4][5][6]. Detection in SFC is mainly performed by UV visible absorption [7] Article Related Abbreviations: ECD, electrochemical detection; FL, fluorescence detection; IS, internal standard; αT, α-tocopherol; αT3, α-tocotrienol; βT, β-tocopherol; βT3, β-tocotrienol; γT, γ-tocopherol; γT3, γ-tocotrienol; δT, δ-tocopherol; δT3, δ-tocotrienol and MS [8,9], but other detection methods have also been applied in SFC to enhance its performance and application.…”

Section: Introductionmentioning

confidence: 99%

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Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers

Yamamoto

Nishimura

Machida

et al. 2022

J of Separation Science

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A supercritical fluid chromatography with electrochemical detection system was developed for the simultaneous determination of tocopherol and tocotrienol isomers. The supercritical fluid chromatography with electrochemical detection system was connected with an additional pump to create a flow path to add a supporting electrolyte solution. The supporting electrolyte solution was mixed with a mobile phase in a post‐column fashion, enabling the independent control of the separation and detection. After optimization of the measurement conditions, vitamin E isomers and an internal standard substance (2,2,5,7,8‐pentamethyl‐6‐hydroxychroman) were separated within 30 min using a mixture of supercritical carbon dioxide and methanol (99:1, v/v) as a mobile phase and a cyanopropyl column (4.6 mm inner diameter × 250 mm length, 5 μm). For the electrochemical detection, methanol containing 1.0 mol/L ammonium acetate was used as a supporting electrolyte solution, and the applied potential was set at +0.8 V. This analytical method showed good linearity (5–100 μg/mL) and repeatability (less than 2.5% relative standard deviation, n = 6) and was applicable to the determination of tocopherol and tocotrienol isomers in nutrition supplements.

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Section: Introductionmentioning

confidence: 99%

“…SFC is a developing chromatographic technique that is expected to provide faster separation with higher resolutions in comparison with HPLC and GC [1–6]. Detection in SFC is mainly performed by UV visible absorption [7] and MS [8, 9], but other detection methods have also been applied in SFC to enhance its performance and application.…”

Section: Introductionmentioning

confidence: 99%

Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers

Yamamoto

Nishimura

Machida

et al. 2022

J of Separation Science

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“…Recently, noteworthy advances in fluid control technology have afforded new developments such as ultrafast separation using SFC. 13,14) Plant cells have several enzymatic and nonenzymatic self-defense mechanisms against oxidation.…”

Section: Introductionmentioning

confidence: 99%

Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

et al. 2022

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We have developed a rapid and sensitive analytical method for α-tocopherol and its oxidative products by combining online hyphenation of supercritical fluid extraction-supercritical fluid chromatography (SFC) with proton transfer reaction (PTR) ionization mass spectrometry (MS). α-Tocopherol is a well-known antioxidant that plays a vital role in the antioxidant defense system in plant cells. However, studies on the cellular mechanisms of α-tocopherol have been limited owing to the lack of a rapid analytical method, which limits the comparison of plant cells incubated in various conditions. Additionally, complex sample preparation and long chromatography separation times are required. Moreover, the majority of the involved molecules are a combination of isomers, which must be separated before applying tandem MS. α-Tocopherol produces the α-tocopheroxyl radical in the first step of its antioxidant function; another ion with the same mass may also be generated from the source. SFC separation effectively distinguished the observed ions from their oxidative products in the sample and those produced during the ionization reaction process. This method enabled the measurement of α-tocopherol and its oxidative products such as α-tocopheroxyl radical and αtocopheryl quinone in approximately 3 min per sample, including the time required for sample preparation.

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Analytical performance evaluation of hemoglobin A1c on an ARKRAY HA-8160 analyzer with newly-developed mobile phase buffer

Yu,

Zhang,

Lin

2024

Practical Laboratory Medicine

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Emerging Separation Techniques in Supercritical Fluid Chromatography

Cited by 5 publications

References 75 publications

Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers

Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers

Rapid Analysis of α-Tocopherol and Its Oxidation Products Using Supercritical Carbon Dioxide and Proton Transfer Reaction Ionization Mass Spectrometry

Analytical performance evaluation of hemoglobin A1c on an ARKRAY HA-8160 analyzer with newly-developed mobile phase buffer

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