Development and Optimization of MicroPropagation, In Vitro Methodology for Gladiolus

Abstract

Gladiolus is a non-native, costly but an important flowering plant in South Asia. Considering its lower production rate and economic importance, micro-propagation technology was optimized in this study to establish a protocol for pathogen free clonal genotypes. Taken the results together, the best response was observed at 27°C as growing temperature, table sugar (sucrose) as a carbon source, apical Keywords BAP IBA Kinetin Micro-propagation MS basal medium NAA meristem of 3mm for shoot formation as an explant. The best plant regeneration was observed for shoot apical meristem (within 7.8 days after inoculation) by using Murashige and Skoog MS basal medium with supplementing 1.0 mg/L BAP and 0.5 mg/L of Kinetin. The best shoot multiplication was 98% in 18.2 days of inoculation using MS media with 1.0 mg/L BAP only. For root induction, MS media which was supplemented with 1.0 mg/L NAA and 2.0 mg/L IBA gave the best results (100% in 6.8 days). The best supporting agent was found to be cotton swab while the best media for hardening and acclimatization of well-developed plants was a combination of sand, soil, and peat in an equal ratio (1:1:1), leading to 80% survival rate of plants. The results obtained in this study may better help grow Gladiolus plants on an industrial scale with a lower cost of production Bioscientific Reviews Volume 1(2) Bioscientific Reviews Volume 1(2)

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

“…Some research reports, however, suggested lower BAP concentrations for shoot regeneration; one such report [94] found that 0.5 mg L-1 BA produced the best results, while [81] obtained better results at concentrations of BAP in the range of 0.5 to 1.0 mg L −1 . Furthermore, increasing BAP concentration from 0.5 to 1.0 mg L −1 increased shooting, while further increases resulted in a low rate of shoot development [80]. Several researchers have reported on NAA alone and its positive influence on in vitro growth [31,54,62,63], as well as in combination with other PGRs, as also reported by [28].…”

“…The surface sterilization of explants was accomplished using a variety of procedures, which differed based on the species of gladiolus and the type of explant. Explants of gladiolus were cleaned with detergents like Teepol, Tween 20, liquid detergent, or savlon, then exposed to sodium hypochlorite (NaOCl) as reported in various works [28,59,79,80]. Numerous research reports also advise applying clorox centramide, dithane M-45, bavistin, antiseptic savlon, dematos with alcohol, and other primary sterilants like HgCl 2 and NaOCl [11,23,35,53,64,66,[81][82][83].…”

“…Various researchers have reported varying and higher concentrations of sugar, up to 5% [31], 7% [29], 12% [108], and 15% sucrose [109], as well as different ranges of sucrose, like 2-12% [22], 3-8% [64], 3-9% [67], and 3-12% [110]. In one report, laboratory sucrose produced 92 percent of shoot formation in 7.6 days, whereas table sugar produced 94 percent of shoot induction in 7.4 days [80]. When sucrose was used in different concentrations (25-50-100 mg L −1 ), Ref.…”

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

“…The sprouted cormlets treated with R 4 took minimum (8.31) days for in-vitro root initiation, which was statistically at par with R 8 (8.61 days), R 7 (8.66 days) and R 5 required the maximum days (10.78 days). The minimum time of root induction (7.2 days) with maximum rooting (96%) was observed with 1.0 mg/L of IBA [15]. Similarly, R 4 produced the maximum number of roots/shoot (5.37) at 30 days after inoculation, which was statistically at par with R 6 (5.34) and R 7 developed the minimum number of roots/shoot (3.65) (Table 2).…”

“…Silva et al [14] found that the regeneration of gladiolus from dormant cormlets suggested the fact regarding breaking of dormancy through hormonal action followed by consequent supply of readily available nutrients suitable to growing tissues from the medium. Induction of shoot bud within 7-8 days 16 and 7.8 days with maximum shooting (96%) [15] was observed in B5 (Gamborg) and MS medium respectively when it was supplemented with 1.0mg/L of BAP. Similar kind of findings was also reported by Budiarto [16] and [17].…”