Development and evaluation of the effect of ethanol and surfactant in vesicular carriers on Lamivudine permeation through the skin

Sudhakar, Kalvatala; Mishra, Vijay; Jain, Sanjay; Rompicherla, Narayana Charyulu; Malviya, Neelesh; Tambuwala, Murtaza M.

doi:10.1016/j.ijpharm.2021.121226

Cited by 24 publications

(16 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For all the suggested combinations, entrapment efficiency was calculated and enlisted in Table 6 . It was noticed that by increasing the amount of lipid entrapment efficiency increases, whereas increasing the concentration of ethanol drug entrapment decreases as shown in Figure 4 d. In this case, also, ANOVA suggests that the model is quadratic in nature having an R 2 value of 0.8443 with a significant F-value for the model and a non-significant P value for the lack of fit [ 43 ].…”

Section: Resultsmentioning

confidence: 97%

Quality by Design Assisted Optimization and Risk Assessment of Black Cohosh Loaded Ethosomal Gel for Menopause: Investigating Different Formulation and Process Variables

et al. 2023

View full text Add to dashboard Cite

Black cohosh (Cimicifuga racemosa) (CR) is a popular herb and is medically lauded for ameliorating myriad symptoms associated with menopause. However, its pharmaceutical limitations and non-availability of a patient-compliant drug delivery approach have precluded its prevalent use. Henceforth, the current research premise is aimed at developing an ethosomal gel incorporating triterpene enriched fraction (TEF) obtained from CR and evaluating its effectiveness through the transdermal application. TEF-loaded ethosomes were formulated using solvent injection, optimized and characterised. The optimized ethosomes were then dispersed into a polymeric gel base to form ethosomal gel which was further compared with the conventional gel by in-vitro and ex-vivo experiments. Here, the quality by design (QbD) approach was exploited for the optimization and development of ethosomal gel. The elements of QbD comprising initial risk assessment, design of experimentation (DoE), and model validation for the development of formulation have all been described in detail. The optimized ethosomes (F03) showed a nanometric size range, negative zeta potential and good entrapment. The in vitro release profile of gel revealed a burst release pattern following the Korsmeyer Peppas model having Fickian diffusion. The transdermal flux of ethosomal gel was observed to be more than that of conventional gel. Texture analysis and rheological characterization of the gel, revealed good strength showing shear thinning and pseudoplastic behaviour. The confocal microscope investigation revealed the deeper skin permeation of ethosomal gel than conventional gel. This result was further strengthened by DSC, IR and histological assessment of the animal skin (Wistar rat), treated with the optimized formulation. Conclusively, the implementation of QbD in the formulation resulted in a better understanding of the process and the product. It aids in the reduction of product variability and defects, hence improving product development efficiencies. Additionally, the ethosomal gel was found to be a more effective and successful carrier for TEF than the conventional gel through the transdermal route. Moreover, this demands an appropriate animal study, which is underway, for a stronger outcome.

show abstract

Section: Resultsmentioning

confidence: 97%

Quality by Design Assisted Optimization and Risk Assessment of Black Cohosh Loaded Ethosomal Gel for Menopause: Investigating Different Formulation and Process Variables

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In vitro skin penetration study of Cur from Cur solutions, Cur-HNGs and Cur-SHNGs on rat skin was implemented using the transdermal diffusion cell systems ( Ali et al, 2021 ; Sudhakar et al, 2021 ). The skin membrane was fixed between the donor chamber and receiving chamber of the diffusion cell, with the epidermis facing the donor chamber and the dermis facing the receiving chamber.…”

Section: Methodsmentioning

confidence: 99%

Silk peptide-hyaluronic acid based nanogels for the enhancement of the topical administration of curcumin

et al. 2022

View full text Add to dashboard Cite

The present study focused on the development of Cur-loaded SOHA nanogels (Cur-SHNGs) to enhance the topical administration of Cur. The physiochemical properties of Cur-SHNGs were characterized. Results showed that the morphology of the Cur-SHNGs was spherical, the average size was 171.37 nm with a zeta potential of −13.23 mV. Skin permeation experiments were carried out using the diffusion cell systems. It was found that the skin retention of Cur-SHNGs was significantly improved since it showed the best retention value (0.66 ± 0.17 μg/cm2). In addition, the hematoxylin and eosin staining showed that the Cur-SHNGs improved transdermal drug delivery by altering the skin microstructure. Fluorescence imaging indicated that Cur-SHNGs could effectively deliver the drug to the deeper layers of the skin. Additionally, Cur-SHNGs showed significant analgesic and anti-inflammatory activity with no skin irritation. Taken together, Cur-SHNGs could be effectively used for the topical delivery of therapeutic drugs.

show abstract

“…Prior to sonication, the creation of vesicular structures and the shape of the produced vesicles were examined using an optical microscope (Labomed inverted microscope, TCM 400, St. Louis, MO, USA). A drop of diluted vesicular suspension was maintained on a clear microscope slide, spread uniformly by putting on the cover slip, and viewed under a 10× magnification optical microscope [ 31 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

Central Composite Design (CCD) for the Optimisation of Ethosomal Gel Formulation of Punica granatum Extract: In Vitro and In Vivo Evaluations

Alam

Shakeel

Foudah

et al. 2022

Gels

View full text Add to dashboard Cite

This research manuscript’s objective was to develop the Punica granatum extract ethosome gel. The use of nanotechnology can improve transdermal drug delivery permeation of its major bioactive compound β-sitosterol. The optimised and developed formulations were further studied in vitro and in vivo. The assessment of the anti-inflammatory activity of the gel was performed in Albino rats. Methanolic extract was prepared and developed into an ethosome suspension and an ethosome gel. To optimise the formulation’s response in terms of particle size (nm) and entrapment efficiency (%), the central composite design (CCD) was used in 22 levels. The effects of factors such as lecithin (%) and ethanol (mL) in nine formulations were observed. Characterisation of ethosome gel was performed and the results showed the particle size (516.4 nm) and mean zeta potential (−45.4 mV). Evaluations of the gel formulation were performed. The results were good in terms of pH (7.1), viscosity (32,158 cps), spreadability (31.55 g cm/s), and no grittiness. In an in vitro study, the percentages of β-sitosterol release of ethosome gel (91.83%), suspension (82.74%), and extracts (68.15%) at 279 nm were recorded. The effects of the formulated gel on formalin-induced oedema in Albino rats showed good results in terms of anti-inflammatory activity. The comparative anti-inflammatory activity of Punica granatum extract and gel showed that the gel action was good for their topical application.

show abstract

Development and evaluation of the effect of ethanol and surfactant in vesicular carriers on Lamivudine permeation through the skin

Cited by 24 publications

References 44 publications

Quality by Design Assisted Optimization and Risk Assessment of Black Cohosh Loaded Ethosomal Gel for Menopause: Investigating Different Formulation and Process Variables

Quality by Design Assisted Optimization and Risk Assessment of Black Cohosh Loaded Ethosomal Gel for Menopause: Investigating Different Formulation and Process Variables

Silk peptide-hyaluronic acid based nanogels for the enhancement of the topical administration of curcumin

Central Composite Design (CCD) for the Optimisation of Ethosomal Gel Formulation of Punica granatum Extract: In Vitro and In Vivo Evaluations

Contact Info

Product

Resources

About