Development and evaluation of a live attenuated camelpox vaccine from a local field isolate of the virus

Abstract

A strain of camelpox virus (CMLV) isolated in the Sudan was attenuated by serial passage in Vero cell monolayers for use as a future vaccine strain. The safety and potency of passage 115 virus (designated Sudan CMLV/115) was tested. Camels inoculated with CMLV/115 showed no clinical disease or skin lesions, developed low-level antibodies and cell-mediated immune response and resisted challenge with virulent wild-type CMLV. Field testing of the candidate vaccine showed that the developed vaccine induces immune response and is safe for young and pregnant camels.

Multiplex PCR for rapid diagnosis and differentiation of pox and pox-like diseases in dromedary Camels

“…These include four reference CMLV strains: VD45 previously supplied by CIRAD-EMVT, France [ 56 ], Dubai camelpox vaccine (Ducapox), and DNAs extracted from purified CMLV-1 and CMLV-14 (kindly provided by Dr. Sophie Duraffour, Rega Institute, leuven, Belgium). Additionally, some previously published CMLVs isolated from outbreaks of the disease in Sudan [ 49 ], CPPV positive specimens collected from previous outbreaks [ 19 ] and skin specimens from an outbreak of papilloma infection in the Sudan [ 2 ], as well as the vaccine strain Sudan CMLV/115 [ 57 ] were also included.…”

Influenza A Virus Infections in Dromedary Camels, Nigeria and Ethiopia, 2015–2017

“…The dromedaries that had influenza A virus RNA–positive nasal swabs were negative for HI antibody. Dromedaries recently infected with a virus are expected to be seronegative for that virus because antibody responses against viruses take around a week to develop ( 9 ), by which time nasal swab specimens are often negative for that virus’s genomic material. Microneutralization tests are more appropriate for testing antibody to contemporary H3N2 viruses ( 10 ); hence, we also tested serum samples for antibody to A/Hong Kong/4801/2014(H3N2) virus using the microneutralization test (Appendix).…”

More cell culture passaged Camelpox virus sequences found resembling those of vaccinia virus

“…10(2): 144-156 145 vaccine based on the isolate Jouf-78 strain produced in Saudi Arabia (Hafez et al, 1992) contains a Listerlike strain of VACV rather than CMLV and a possible contamination event during production may have caused this mistaken identity. Previously, the development of a live attenuated CMLV vaccine by passage of a Sudanese isolate in Vero cells has been reported by Abdellatif et al (2014). The present study was prompted by the interesting findings of Yousif and Al Ali (2012) on the mistaken identity of Al Jouf-78 CMLV vaccine.…”

“…Previously, the development of a live attenuated CMLV vaccine by passage of a Sudanese isolate in Vero cells has been reported by Abdellatif et al (2014) .…”