Development and clinical application of high performance liquid chromatography for the simultaneous determination of plasma levels of theophylline and its metabolites without interference from caffeine

Kizu, Junko; Watanabe, Shigekazu; Yasuno, Nobuhiro; Arakawa, Yoshihiro; Uzu, Sonoko; Kanda, Susumu; Komoda, Fusako; Iwata, Tadahisa; Hayakawa, Hiroshi; Hayakawa, Tetsuo; Imai, Kazuhiro

doi:10.1002/(sici)1099-0801(199902)13:1<15::aid-bmc766>3.0.co;2-d

Cited by 23 publications

(5 citation statements)

References 10 publications

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“…The concentration of unchanged theophylline in plasma was measured based on the method reported by Kizu et al 18 with minor modifications. First, 300 μL of acetonitrile, containing 0.3 μg of β‐hydroxyethyltheophylline as an internal standard (IS), was added to 100 μL of a plasma sample.…”

Section: Methodsmentioning

confidence: 99%

Effect of St. John's Wort on the Pharmacokinetics of Theophylline in Healthy Volunteers

Morimoto

Kotegawa

Tsutsumi

et al. 2004

The Journal of Clinical Pharma

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The objective of this study was to investigate the effect of St. John's wort (SJW, Hypericum perforatum) on the pharmacokinetics of theophylline in healthy volunteers. Twelve healthy Japanese male volunteers participated in this randomized, open-labeled, crossover study. The subjects took an SJW caplet (300 mg) three times a day for 15 days. On day 14, they received a single oral dose of 400 mg of theophylline. They took the same dose of theophylline without SJW treatment on another occasion. Plasma and urine samples were obtained during a 48-hour period after theophylline administration. Theophylline concentrations in plasma and urine, as well as the major metabolites (13U, 1U, 3X) in urine, were measured. SJW caused no significant changes in the pharmacokinetics of theophylline in plasma. SJW administration tended to increase the ratio of 1U/the total amount excreted in urine. However, no changes in the ratio of unchanged theophylline, 13U, and 3X were observed. It is unlikely that the effect of 15 days of treatment with SJW on CYPs is sufficient to cause a change in plasma theophylline concentrations.

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Section: Methodsmentioning

confidence: 99%

Effect of St. John's Wort on the Pharmacokinetics of Theophylline in Healthy Volunteers

Morimoto

Kotegawa

Tsutsumi

et al. 2004

The Journal of Clinical Pharma

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“…Among the reported methods for the determination of theophylline in plasma, such as fluorescent polarized immunoassay (Rojanasthien et al, 2001), chemiluminescent immunoassay (Malliaros et al, 1997), HPLC (Schrieber-Deturmeny and Bruguerolle, 1996;Umemura et al, 1998;Kizu et al, 1999), LC-MS (Watson et al, 2001;Kanazawa et al, 2000), HPLC is the most common used one. In this study, an HPLC method was used to determine the pharmacokinetics of TGS SR tablets with the new asymmetric regimen, which was previously developed and validated in our group and used to study the single-and multiple-dose pharmacokinetics of TSG SR tablets (Yao et al, 2000).…”

Section: Methods Development and Validationmentioning

confidence: 99%

Pharmacokinetics of a new sustained‐release formulation of theophylline sodium glycerinate in healthy subjects with a new asymmetric dosage regimen

Wang

Zhong

et al. 2003

Biomedical Chromatography

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Pharmacokinetics of a new sustained-release formulation of theophylline sodium glycerinate in healthy subjects was studied. In this study, a new asymmetric dosage regimen was presented to achieve a better accordance with the chronotherapy of asthma. Each of 10 subjects was administered one tablet (equivalent to 0.1 g anhydrous theophylline) in the morning and four tablets in the evening for a consecutive 6 days and blood samples were collected at the predetermined time and analyzed by a validated HPLC method. This new regimen produced a steady and effective level of theophylline in plasma for the whole day, especially in the evening. A lower dose in the morning could reach the effective level (C(min)4.97 +/- 1.60 microg/mL and C(max)10.68 +/- 1.80 micro g/mL over the a.m. dosing interval) and a higher dose in the evening did not result in toxic levels but led to a reasonable concentration range (C(max)9.72 +/- 1.56 microg/mL over p.m. dosing interval), which could maintain a higher plasma theophylline concentration without the risk of serious adverse events and control asthmatic symptoms probably occurring during the night or early in the morning. The results suggested that the proposed asymmetric regimen was necessary, practicable and safe for twice daily sustained-release tablets of theophylline sodium glycerinate and also provides the basis for the clinical dosage regimen of other theophylline formulated products.

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“…Several high-performance liquid chromatography (HPLC)-based bioanalytical methods have been published for the quantitation of theophylline [3][4][5]. Most of these published methods suffer from long HPLC run times (over 60 min) and high detection limits (greater than 300 ng/mL for theophylline metabolites).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a sensitive LC–MS/MS method for the simultaneous quantitation of theophylline and its metabolites in rat plasma

Chae

Kim

Lee

et al. 2012

Journal of Chromatography B

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Development and clinical application of high performance liquid chromatography for the simultaneous determination of plasma levels of theophylline and its metabolites without interference from caffeine

Cited by 23 publications

References 10 publications

Effect of St. John's Wort on the Pharmacokinetics of Theophylline in Healthy Volunteers

Effect of St. John's Wort on the Pharmacokinetics of Theophylline in Healthy Volunteers

Pharmacokinetics of a new sustained‐release formulation of theophylline sodium glycerinate in healthy subjects with a new asymmetric dosage regimen

Development and validation of a sensitive LC–MS/MS method for the simultaneous quantitation of theophylline and its metabolites in rat plasma

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