Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR

Merdinoglu, Didier; Butterlin, Gisèle; Bévilacqua, Lucie; Chiquet, V.; Adam-Blondon, Anne-Françoise; Decroocq, Stéphane

doi:10.1007/s11032-004-7651-0

Cited by 206 publications

(114 citation statements)

References 37 publications

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“…Extracted DNA was quantified using a Nanodrop spectrophotometer and the concentrations were standardised to fall within the range 25-35 ng/ll. SSRs were selected from the literature and the NCBI database (Thomas and Scott 1993;Bowers et al 1996Bowers et al , 1999Scott et al 2000;Decroocq et al 2003;Adam-Blondon et al 2004;Di Gaspero et al 2005;Merdinoglu et al 2005;Doligez et al 2006;Cipriani et al 2008; http://www.ncbi.nlm.nih.gov/) with the aim to achieve comprehensive genome coverage and increased marker density for known minor and major mildew resistance QTL regions on chromosomes 5, 12, 15 and 18. A standard set of PCR conditions (1.8 mM MgCl 2 , 0.75 U Supertherm Taq, 5 mM dNTP and 0.3 pmol/ll of each primer) was used for all reactions.…”

Section: Molecular Analysismentioning

confidence: 99%

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

et al. 2014

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One hundred and eighty six F 1 plants from a 'Regent' 9 'RedGlobe' cross were used to generate a partial linkage map with 139 microsatellite markers spanning all 19 chromosomes. Phenotypic scores for downy mildew, taken over two years, confirmed a major resistance QTL (Rpv3) against downy mildew in the interval VVIN16-cjvh to UDV108 on chromosome 18 of 'Regent'. This locus explained up to 62 % of the phenotypic variance observed. Additionally a putative minor downy mildew resistance locus was observed on chromosome 1 in one season. A major resistance locus against powdery mildew (Ren3) was also identified on chromosome 15 of 'Regent' in the interval UDV116 to VChr15CenGen06. This study established the efficacy of and validated the 'Regent'-derived downy and powdery mildew major resistance genes/QTL under South African conditions. Closely linked SSR markers for marker-assisted selection and gene pyramiding strategies were identified.

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Section: Molecular Analysismentioning

confidence: 99%

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

et al. 2014

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“…Efficient use of microsatellites should also be increased by the availability of a larger set of new microsatellite markers suitable for multiloading and multiplexing, respectively [6]. In addition, microsatellites could be used for genetic uniformity detection in grapevine plants propagated in vitro [27] and plant origin certification.…”

Section: Discussionmentioning

confidence: 99%

“…They have been applied in grapevine genetic map construction, genetic diversity assessment, pedigree studies, and clonal pre-selection [5,7,8]. Suitable microsatellite primers for multiplex assays have also been developed [6].…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Dokupilová

Migliaro²,

Mihálik

et al. 2014

Open Life Sciences

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Microsatellites were used as a very effective tool for genetic diversity analysis and characterization of 51 grapevine (Vitis vinifera L.) accessions from the national collection of genetic resources. Genetic diversity was relatively high, 8.91 alleles were detected per analysed microsatellite locus in average, and fifty-one accessions were distinguished into 45 groups. Distribution of recent Slovak cultivars across the dendrogram accented both their genetic diversity and the effectiveness of the national breeding program in maintaining genetic diversity and generating new genetic variants. Each cultivar was different from the others and twelve of them contained 77.6% of the total genetic diversity of the whole analysed set. Microsatellite patterns were also able to confirm parentage in selected Slovak cultivars. An unusual phenomenon of triallelism was also detected in one of the analysed accessions. The present study has initiated molecular characterization within the national grapevine genetic resource collection and their comparison with well-established international cultivars.

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“…We used 468 pairs of SSR primers, including the VMC series, the VVS series (Thomas and Scott, 1993), the VVMD series (Bowers et al, 1996(Bowers et al, , 1999, the VrZAG series (Sefc et al, 1999), the VVI series (Merdinoglu et al, 2005), the UDV series (Di Gaspero et al, 2005), the Chr series (Blasi et al, 2011), and the VLG series, which were obtained from genomic sequence information. The PCR volume was 16 μL, which contained 10 ng DNA, 2.0 mM Mg 2+ , 100 μM dNTPs, 0.3 μM primer, 0.8 U Taq DNA polymerase, and 1X PCR buffer.…”

Section: Ssr Primers and Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

Quantitative trait locus analysis of grape weight and soluble solid content

Zhao¹,

Guo²,

Lin³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar '87-1' and the late-maturing male parent cultivar '9-22'. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequencerelated amplified polymorphism and simple-sequence repeat marker analysis were conducted we constructed molecular genetic maps of the parents. The molecular linkage map of '87-1' had 19 linkage groups that contained 188 markers, with an average interval of 5.7 cM and a total distance of 1074.5 cM; the '9-22' map had 19 linkage groups that contained 175 markers, with an average interval of 7.8 cM and a total distance of 1100.2 cM. The molecular linkage map of both parents had 19 linkage groups that contained 251 markers, with an average interval of 5.0 cM and a total distance of 1264.2 cM. We used the interval mapping method to conduct a quantitative trait locus (QTL) analysis 9873 QTL analysis of grape weight and soluble solid content ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9872-9881 (2015) of grape weight and soluble solid content of the mapping population. Six QTLs were related to grape weight, and the average contribution to the phenotypic variance was between 11.3 and 33.0%. Seven QTLs were related to soluble solid content, and the average contribution to the phenotypic variance was between 15.7 and 55.8%.

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Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR

Cited by 206 publications

References 37 publications

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Quantitative trait locus analysis of grape weight and soluble solid content

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