Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Dokupilová, Ivana; Migliaro, Daniele; Mihálik, Daniel; Crespan, Manna; Kraic, Ján

doi:10.2478/s11535-014-0314-1

Cited by 2 publications

(2 citation statements)

References 23 publications

(25 reference statements)

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“…The PI or the Probability of identity is the probability that two individuals of a population or sample have the same genotype by chance and not by kinship (MIRIMIN et al, 2015). This is the most used theoretical estimator to access the relied statistical for individual identification and to quantify the levels of genetic diversity in populations or samples (WAITS;LUIKART;TABERLET, 2001;DOKUPILOVÁ et al, 2014;MOLLET et al, 2015). According to Hvarleva et al (2007), average PI values higher than 0.546 are associated to a low amount of identified alleles.…”

Section: Resultsmentioning

confidence: 99%

Use of molecular markers SSR and SCAR for identification of olive accessions

et al. 2020

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Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6. The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively. The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species.

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Section: Resultsmentioning

confidence: 99%

Use of molecular markers SSR and SCAR for identification of olive accessions

et al. 2020

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“…They suggested that the probability of finding two individuals with the same genotype was very close to zero, considering both the marker set and the sample size utilized. However, the PI is considered the most widely used theoretical estimator for accessing supporting statistics for individual identification and quantifying the level of genetic variability in populations or samples ( Dokupilová et al, 2014 ; Mollet et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Assessment of SSR and RAPD markers for genetic diversity in some Mango cultivars

A. A. Hussein,

Eid,

Rahimi

et al. 2023

PeerJ

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Genetic improvement mainly depends on the level of genetic variability present in the population, and the degree of genetic diversity in a population largely determines the rate of genetic advancement. For analyzing genetic diversity and determining cultivar identities, a molecular marker is a useful tool. Using 30 SSR (simple sequence repeat) and 30 RAPD (randomly amplified polymorphic DNA) markers, this study evaluated the genetic divergence of 17 mango cultivars. The effectiveness of the two marker systems was evaluated using their genetic diversity characteristics. Additionally, the effects of SM (simple matching) and Dice similarity coefficients and their effects on mango clustering were evaluated. The findings showed that SSR markers generated 192 alleles, all of which were polymorphic (100%). With RAPD markers, 434 bands were obtained, 361 of which were polymorphic (83%). The average polymorphic information content (PIC) for RAPD and SSR was 0.378 and 0.735, respectively. Using SSR markers resulted in much higher values for other genetic diversity parameters compared to RAPD markers. Furthermore, grouping the genotypes according to the two similarity coefficients without detailed consideration of these coefficients could not influence the study results. The RAPD markers OPA_01, OPM_12 followed by OPO_12 and SSR markers MIAC_4, MIAC_5 followed by mMiCIR_21 were the most informative in terms of describing genetic variability among the cultivars under study; they can be used in further investigations such as genetic mapping or marker-assisted selection. Overall, ‘Zebda’ cultivar was the most diverse of the studied cultivars.

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Genotyping of Vitis vinifera L. within the Slovak national collection of genetic resources

Cited by 2 publications

References 23 publications

Use of molecular markers SSR and SCAR for identification of olive accessions

Use of molecular markers SSR and SCAR for identification of olive accessions

Comparative Assessment of SSR and RAPD markers for genetic diversity in some Mango cultivars

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