Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

Heerden, Carel van; Burger, Phyllis; Vermeulen, Abraham; Prins, R.

doi:10.1007/s10681-014-1167-4

Cited by 69 publications

(55 citation statements)

References 66 publications

(134 reference statements)

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“…In the maternal maps, a region on chromosome 15 exhibited significant segregation distortion, an observation previously reported by Lowe and Walker (2006), Welter et al (2007), Riaz et al (2011), Heerden et al (2014) and Guo et al (2014). In contrast, the paternal maps (Figs.…”

Section: Discussionsupporting

confidence: 59%

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

et al. 2016

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Quantitative trait locus (QTL) identification in perennial fruit crops is impeded largely by their lengthy generation time, resulting in costly and labor-intensive maintenance of breeding programs. In a grapevine (genus Vitis) breeding program, although experimental families are typically unreplicated, the genetic backgrounds may contain similar progenitors previously selected due to their contribution of favorable alleles. In this study, we investigated the utility of joint QTL identification provided by analyzing half-sib families. The genetic control of powdery mildew was studied using two half-sib F1 families, namely GE0711/1009 (MN1264 × MN1214; N = 147) and GE1025 (MN1264 × MN1246; N = 125) with multiple species in their ancestry. Maternal genetic maps consisting of 1077 and 1641 single nucleotide polymorphism (SNP) markers, respectively, were constructed using a pseudo-testcross strategy. Ratings of field resistance to powdery mildew were obtained based on whole-plant evaluation of disease severity. This 2-year analysis uncovered two QTLs that were validated on a consensus map in these half-sib families with improved precision relative to the parental maps. Examination of haplotype combinations based on the two QTL regions identified strong association of haplotypes inherited from ‘Seyval blanc’, through MN1264, with powdery mildew resistance. This investigation also encompassed the use of microsatellite markers to establish a correlation between 206-bp (UDV-015b) and 357-bp (VViv67) fragment sizes with resistance-carrying haplotypes. Our work is one of the first reports in grapevine demonstrating the use of SNP-based maps and haplotypes for QTL identification and tagging of powdery mildew resistance in half-sib families.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-016-0586-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 59%

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

et al. 2016

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“…In grapevine, QTL mapping experiments proved that major R genes activate ETI, explaining from 49% to 78.9% of the observed phenotypic variance. The same experiments did not detect stable QTLs for the remaining genetic component of the phenotypic variance observed in resistant progeny (Bellin et al., ; van Heerden, Burger, Vermeulen, & Prins, ; Revers et al., ; Schwander et al., ; Venuti et al., ; Welter et al., ). We hypothesize that full‐sib progeny provide a too narrow range of variation in genetic backgrounds to unleash statistically significant effects and explain the apparent variation in ETI efficacy.…”

Section: Discussionmentioning

confidence: 88%

The genetic background modulates the intensity of Rpv3‐dependent downy mildew resistance in grapevine

et al. 2018

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Grape varieties with resistance to downy mildew (DM) carry alien chromosome segments in Vitis vinifera backgrounds. We previously showed that the largest descent group shares a non‐vinifera haplotype at the locus Rpv3. Here, we performed a common garden experiment with 76 varieties to evaluate the level of field resistance across four years. All varieties exhibited effector‐triggered immunity (ETI)‐associated necrosis. On a scale of 1–9, the median OIV452 value for foliar resistance was 7.1 in the resistant lineage vs. 3.2 in vinifera controls. Genotype, year and their interaction significantly affected the level of resistance. Some resistant genotypes showed high mean values of OIV452 and low variance among years. Other resistant genotypes showed lower mean OIV452 and higher variance. They were capable of activating ETI, but the intensity was inadequate to restrict pathogen growth under highly conducive conditions. Rpv3‐dependent responses were stronger in highly native genetic backgrounds and tended to attenuate in late backcross generations. Genetic backgrounds donated by European winegrapes of the convarietas occidentalis provided on average higher levels of Rpv3 resistance than backgrounds of orientalis table grapes.

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“…The SSR markers chosen for each objective varied, depending on whether a genome scan covering all or selected linkage groups was required, or whether a specific QTL region was targeted. The SSR markers used to screen the 'Villard Blanc' populations were selected from Van Heerden et al (2014), whilst the 'Kishmish Vatkana' markers were selected from Hoffmann et al (2008) and the National Centre for Biotechnology Information (NCBI) Map Viewer webpage (www.ncbi. nlm.nih.gov).…”

Section: Molecular Analysismentioning

confidence: 99%

“…In an attempt to achieve durable resistance, the general hypothesis is that the combination of more than one resistance locus in a single cultivar, which is called gene pyramiding, will ensure that pathogens do not easily overcome the respective resistance genes/QTL (Eibach et al, 2007;Iwata et al, 2016). Extensive research has been done in order to identify the resistance genes or QTL in known resistant varieties and to identify molecular markers linked to these loci, for MAS to improve selection efficiency (Bellin et al, 2009;Barba et al, 2014;Van Heerden et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Confirmation of the effectiveness and genetic positions of disease resistance loci in ‘Kishmish Vatkana’ (Ren1) and ‘Villard Blanc’ (Ren3 and Rpv3)

Veikondis¹,

Burger²,

Vermeulen³

et al. 2018

sajev

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This study aimed to validate the effectiveness and to genetically characterise the fungal disease resistance genes of 'Kishmish Vatkana' and 'Villard Blanc' in South Africa using microsatellite (SSR) markers and a Quantitative Trait Loci (QTL) approach. An F 1 'Sunred Seedless' × 'Kishmish Vatkana' cross was used to generate a partial linkage map for chromosome 13 known to harbour the Ren1 powdery mildew locus of 'Kishmish Vatkana'. The effectiveness of this locus was validated, explaining between 44.8% and 57.7% of the observed phenotypic variance. An F 1 'Villard Blanc' × 'G1-6604' cross was used to generate partial linkage maps for chromosomes 15 and 18, reported to harbour fungal resistance genes of 'Villard Blanc'. The powdery mildew QTL (Ren3) was validated on chromosome 15 of 'Villard Blanc', which explained between 18.9% and 23.9% of the phenotypic variance observed. The downy mildew resistance QTL on chromosome 18 (Rpv3) of 'Villard Blanc' was also confirmed, and it explained between 19.1% and 21.2% of the phenotypic variance observed. This molecular information and individual sources of resistance have already been implemented in the marker-assisted selection (MAS) and gene pyramiding efforts of the table grape breeding program of the Agricultural Research Council (ARC) Infruitec-Nietvoorbij.

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Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

Cited by 69 publications

References 66 publications

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

Genetic dissection of powdery mildew resistance in interspecific half-sib grapevine families using SNP-based maps

The genetic background modulates the intensity of Rpv3‐dependent downy mildew resistance in grapevine

Confirmation of the effectiveness and genetic positions of disease resistance loci in ‘Kishmish Vatkana’ (Ren1) and ‘Villard Blanc’ (Ren3 and Rpv3)

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