Development and characterisation of an enzyme electrode for the application to undiluted media

Pfeiffer, D.; Setz, K.; Klimes, N.; Schulmeister, Thomas; Scheller, Frieder W.

doi:10.1016/b978-1-85617-161-8.50027-6

Cited by 3 publications

(3 citation statements)

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“…An amperometric lactate biosensor was described by Pfeiffer and co-workers [69]. A suspension of lactate oxidase and bovine serum albumin in acetonic polyurethane solution was spread onto a cellulose membrane.…”

Section: Immobilization Of Biocomponentsmentioning

confidence: 99%

Use of polymer films in amperometric biosensors

1995

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Different types of polymeric films implemented in various capacities in amperometric biosensor design are reviewed. Conducting and nonconducting polymer films and composite films are considered. Methods of film fabrication such as solvent casting, electropolymerization, and adsorption are presented. Film function, including prevention of interference and fouling problems, immobilization of the enzyme or active biocomponent, extension of linear range, or to address biocompatibility problems are described. The use of polymeric films as a medium to incorporate mediators, while an important function, is too vast for review here. The applications of polymer film-modified biosensors for selected analytical determinations are also presented. This review focuses upon the use of polymer films in amperometric biosensors and emphasizes innovative biosensor designs and unique applications, and omits traditional free-standing films unless they are coupled with new types of polymer films or used innovatively.

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Section: Immobilization Of Biocomponentsmentioning

confidence: 99%

Use of polymer films in amperometric biosensors

1995

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“…Several other amperometric sensors for creatinine determination have been proposed. − All of these biosensors are based on the enzyme catalytic sequence introduced by Tsuchida and Yoda: 8 where CA = creatinine amidohydrolase, CI = creatine amidinohydrolase, SO = sarcosine oxidase.…”

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confidence: 99%

Miniaturized Biosensors Employing Electropolymerized Permselective Films and Their Use for Creatinine Assays in Human Serum

1996

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Miniaturized, disposable amperometric biosensors for determination of creatinine in human serum are described. The base electrodes are fabricated using micro-electronics techniques, to build a multilayer film structure on a polyimide foil. By using a thin electropolymerized film of poly(1,3-diaminobenzene), the electrochemical interferences from ascorbate, urate, acetaminophen, and other oxidizable species are greatly diminished. The multienzyme system (creatininase, creatinase, sarcosine oxidase) is immobilized on top of the permselective layer using cross-linking of the proteins with glutaraldehyde. The electropolymerization conditions for obtaining almost ideal permselectivity of the inner layer are defined, as well as the optimal enzyme layer preparation. A composite polymeric outer membrane [Nafion + poly-(2-hydroxy-ethyl methacrylate) is used for diffusion control and to protect the enzyme layer from fouling. The reagentless planar sensors for creatinine and creatine have fast response time (t95 = 1 min), linear response up to 1.2 mM in batch-type and 2.0 mM in flow injection analysis and a detection limit of 10-20 muM. They are applied in a differential setup for creatinine assay in control and hospital human serum samples and are suitable for incorporation in a portable analyzer.

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“…Lactate-sensitive amperometric bioelectrodes, based primarily on lactate oxidase (LOX) or lactate dehydrogenase (LDH) are widely used for these applications [ 1-71, Various immobilization schemes have been used, in connection with different electrode transducers, for fabricating these lactate probes. These include the entrapment of LDH [1,2] or LOX [3] behind different (dialysis or Nylon) membranes. or of LOX within an electropolymerized film [4]; the incorporation of LOX and methylene-green mediator within a carbon paste matrix [ 51; the adsorption of LOX and tetrathiafulvalene onto carbon foil [6]; or the immobilization of LDH [7] and LOX [8] onto carbon fiber surfaces.…”

Section: Introductionmentioning

confidence: 99%

Lactate biosensor based on a lactate dehydrogenase/nicotinamide adenine dinucleotide biocomposite

Wang

Chen

1994

Electroanalysis

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A new biocomposite. fabricated by incorporating lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide (NAD+) within a graphite-epoxy matrix, is used as a polishable (renewable) and robust biosensor for lactate. Highly sensitive and rapid amperometric biosensing is accomplished at relatively low potentials. The regeneration capability of the biocomposite probe circumvents problems of surface fouling inherent to the detection of NADH. Flom--injection detection of lactate, with a sample throughput of 6O/h, detection limit of 5 X lo-' hI. precision of 2.5% (RSD for n = 40), and linearit). up to 2 x lo-' M, is illustrated.

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Development and characterisation of an enzyme electrode for the application to undiluted media

Cited by 3 publications

References 0 publications

Use of polymer films in amperometric biosensors

Use of polymer films in amperometric biosensors

Miniaturized Biosensors Employing Electropolymerized Permselective Films and Their Use for Creatinine Assays in Human Serum

Lactate biosensor based on a lactate dehydrogenase/nicotinamide adenine dinucleotide biocomposite

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