Development and application of a SRAP marker for the identification of sex in Buchloe dactyloides

Zhou, Yingjie; Wang, Xianguo; Zhang, Xinquan

doi:10.1007/s10681-011-0419-9

Cited by 23 publications

(18 citation statements)

References 49 publications

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“…SRAP markers are more powerful for revealing genetic diversity among closely related cultivars than SSR, ISSR, or RAPD markers (Budak et al 2004) and can be used for linkage map construction (Yeboah et al 2007), genomic and cDNA fingerprinting, gene tagging (Li and Quiros 2001), genetic diversity analysis (Li et al 2009), map-based cloning (Zhang et al 2010), hybrid identification (Mishra et al 2011) and sex determination (Zhou et al 2011). In our study, SRAP markers were used for the first time to analyse genetic diversity in important cultivars and advanced breeding lines of mungbean varying in micronutrient (Fe and Zn) content.…”

Section: Introductionmentioning

confidence: 99%

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

Aneja

Yadav

et al. 2013

Physiol Mol Biol Plants

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Vigna radiata (L.) Wilczek, commonly called mungbean is an important pulse crop. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic variability in the available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study molecular diversity using Sequence-related amplified polymorphism (SRAP) among 21 Vigna radiata genotypes. Twenty nine SRAP primer combinations produced a total of 121 amplified bands which were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from 70 bp to 3,000 bp and 6 out of 29 SRAP primers were most useful in fingerprinting Vigna radiata genotypes under study. The similarity coefficients between different genotypes ranged from 0.45 to 0.96 with an average similarity value of 0.71. At an arbitrary cut-off at 60 % similarity level on a dendrogram, the Vigna radiata accessions were categorized into two major clusters. ML1108 and 2KM115 were found to be genetically similar. SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. Mapping population involving ML776 and Satya could be used for tagging gene(s) for micronutrient content. The results indicated that SRAP markers were efficient for identification of Vigna radiata genotypes and assessment of the genetic relationships among them.

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Section: Introductionmentioning

confidence: 99%

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

Aneja

Yadav

et al. 2013

Physiol Mol Biol Plants

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“…Engelm, a dioecious species native to North America (Beetle, 1950;Huff and Wu, 1987;Budak, 2004), has become the most widely cultivated warm-season grass in Northern China. Buffalograss produces female and male flowers, which are produced on separate unisexual individuals (Zhou et al, 2011). Furthermore, the inflorescences and spikelets are also sexually dimorphic, which is similar to the female and male plants of Bouteloua dimorpha (Kinney et al, 2008).…”

Section: Introductionmentioning

confidence: 86%

Changes in inflorescence protein during advanced stages of floret development in Buchloe dactyloides (Poaceae)

Zhou¹,

Xue²,

Wang³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Buffalograss, Buchloe dactyloides, is a dioecious species native to the Great Plains of North America. The florets at the early stages of development possess both gynoecium and androecium organ primordia but later become unisexual. Very little is known about the proteomic changes that occur when the florets change from hermaphroditism to unisexuality. We compared the protein composition of florets at the hermaphroditic stage with that at the unisexual stage. The development stage of the floret was determined by stereomicroscopic observation. Two-dimensional gel electrophoresis was used to separate the proteins extracted from female and male inflorescences. Stagespecific protein maps, with an average of about 400 spots per map, were analyzed with the protein analysis software. Eighteen spots were found to be differentially expressed between the hermaphrodite and unisexual stages. Of these, 12 were present at both stages but with a different expression value. Four specific spots appeared at the hermaphrodite stage and disappeared at the unisexual stage. Two specific protein spots were associated with female and male floret differentiation. One appears to be associated with contabescence in the female floret and the final protein appears to lead to the abortion of gynoecium in the male floret. The MALDI TOF/TOF technique was used for peptide mass fingerprinting of the differentially expressed proteins and the MASCOT software was used to search the protein database. However, only two protein spots were identified from the database. These were aldolase1 and Os05g0574400 (similar to malate dehydrogenase). This type of proteomic study can help to identify novel protein products and determine the mechanisms involved in the floral sex differentiation process in buffalo grass.

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“…Many dioecious plants have been successfully used for studying sexual determination, such as Asparagus officinalis L. (Jiang and Sink, 1997), Eucommia ulmoides Oliv. , and Buchloe dactyloides (Zhou et al, 2011), among others.…”

Section: Introductionmentioning

confidence: 99%

“…SSR is one of the most widely used marker systems because of its extensive dispersion around the genome and because it produces co-dominant markers, but it is expensive to develop. Although, RAPD is the most widely used molecular marker for sex identification, its low reproducibility limits its effectiveness (Zhou et al, 2011). These techniques generate relatively complex patterns for identification purposes and make the analysis of DNA fragments tedious and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

Wang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequencerelated amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify. A total of 342 primer combinations were screened and 2770 bands were examined. Only me14/em8 could amplify a specific fragment (210 base pairs) in all female but none in male plants. We analyzed this fragment using GenBank and found that the sequence similarity was 80% to the Populus trichocarpa clone POP006-H09 (sequence ID: gb|AC212923.1|) and that of the deduced amino acid sequence was 73% to the integrase of Mendicago truncatula (sequence ID: gb|ABD28291.1|) and 71% to the predicted retrotransposon integrase-like protein 1-like in Cicer arietinum (sequence ID: ref|XP 004515460.1|) (NCBI database through December 17, 2013). This fragment was converted into a stable and simple sequencecharacterized amplified region marker approximately 200 base pairs in length. This marker can be utilized for early sexual identification in I. polycarpa, which will facilitate future breeding programs.

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Development and application of a SRAP marker for the identification of sex in Buchloe dactyloides

Cited by 23 publications

References 49 publications

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

Changes in inflorescence protein during advanced stages of floret development in Buchloe dactyloides (Poaceae)

Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

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