Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

Abstract

ABSTRACT. Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequencerelated amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify. A total of 342 primer combinations were screened and 2770 bands were examined. Only me14/em8 could amplify a specific fragment (210 base pairs) in all female but none in male plants. We analyzed this fragment using GenBank and found that the sequence similarity was 80% to the Populus trichocarpa clone POP006-H09 (sequence ID: gb|AC212923.1|) and that of the deduced amino acid sequence was 73% to the integrase of Mendicago truncatula (sequence ID: gb|ABD28291.1|) and 71% to the predicted retrotransposon integrase-like protein 1-like in Cicer arietinum (sequence ID: ref|XP 004515460.1|) (NCBI database through December 17, 2013). This fragment was converted into a stable and simple sequencecharacterized amplified region marker approximately 200 base pairs in length. This marker can be utilized for early sexual identification in I. polycarpa, which will facilitate future breeding programs.

Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers

“…Prior to our study, SRAP markers have been used for sex identification only in few plants. Female sex specific SRAP markers have been identified in Buchloe dactyloides [20], Idesia polycarpa [21], Ficus pumila [74] and Litsea cubeba [75].…”

“…Different PCR based dominant markers such as ISSR, SCoT, SRAP, etc. have been effectively used for quantification of genetic diversity [16, 17, 18, 19] and sex identification in different plants [20, 21]. In the present study, ISSR and SCoT markers were employed to analyze genetic diversity and phylogenetic relationships in 52 T. dioica accessions belonging to nine different populations of India and for sex identification, SRAP markers were used.…”

The use of soil pollen to determine the sex of overhead individuals of a temperate dioecious shrub

“…Besides using flowering and fruiting, sex chromosomes and genetic markers have been used to determine the sex of individuals for some species (e.g., Joseph et al, 2014; Wang et al, 2015; Wu et al, 2015). One major advantage of these methods is that the sex can be determined for prereproductive individuals, as early as seedlings (e.g., Joseph et al, 2014; Sun et al, 2014).…”

Analysis of Chemical Composition and Antioxidant Activity of Idesia polycarpa Pulp Oil from Five Regions in China

“…Idesia polycarpa , a deciduous tree of the Flacourtiaceae family, is native to China, Korea, Japan, and Russian Far East [ 3 ]. In China, I. polycarpa is mainly distributed south of the Qinling Mountains and the Huaihe River [ 4 ]. The plant was formerly used as an ornamental plant in China because of its strong adaptability and attractive appearance [ 5 ].…”