Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers

Kumar, Jatin; Agrawal, Veena

doi:10.1016/j.heliyon.2019.e01346

Cited by 39 publications

(19 citation statements)

References 55 publications

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“…As shown in Figure (5) the generated dendrogram from the combined results of ISSR and SCoT markers after the cluster analysis; resembles somehow the dendrogram generated after the ISSR marker. Kumar and Agrawal (2019) obtained the similar conclusion on the superiority of the ISSR marker (95.96% polymorphism) over the SCoT marker (92.20% polymorphism) when analyze genetic diversity and population structure among 52 Trichosanthes dioica Roxb. Previously the ISSR marker have been approved to be the more discriminating marker for the genetic diversity between Hordeum vulgre cultivars and landraces compared with RAPD and SSR Osman (2016).…”

Section: Discussionsupporting

confidence: 57%

Assessment of genetic diversity among some fennel cultivars (Foeniculum vulgare Mill.) by ISSR and SCoT Markers

Ramadan

Shoaib

Ali

et al. 2019

African Journal of Biological Sciences

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Fennel (Family Apiaceeae) is one of those strategic medicinal and aromatic plants in Egypt with great value as an economical exported plant. Fennel undergoes propagation through breeding of some fennel cultivars from different world region in order to keep out of genetic deterioration in the native Egyptian cultivars, and enrich its favorable active ingredients. The advanced plant breeding programs depend on some markers such as morphological, cytological, biochemical and molecular markers to study its genetic diversity. In this work two molecular markers ISSR and SCoT markers were used to assessment of genetic diversity among six fennel cultivars (Foeniculum vulgare Mill.) planted in Egypt; three of Egyptian origin (Balady, Elalamy and Azoricum) and the other ones are of different origin (Holland, Indian and German). The analyzed data reveals that the ISSR marker is more discriminating, provides more informative data than SCoT marker and can be reliable to evaluate the genetic diversity among the six examined cultivars as it performs the highest percentage of polymorphism 61% and higher unique bands number (4). According to ISSR molecular marker German and Indian cultivars are close distant; can exchange gene in between them smoothly despite the ultimate change in the environmental and weather conditions of their origin land. In conclusion, according to Nie , s and li , s coefficient based on SCoT marker analysis between the cultivars; Indian cultivar was quite distant from Azoricum by the same time that Azoricum is close distant to Holland, as well as; Elalamy is close distant to Balady.

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Section: Discussionsupporting

confidence: 57%

Assessment of genetic diversity among some fennel cultivars (Foeniculum vulgare Mill.) by ISSR and SCoT Markers

Ramadan

Shoaib

Ali

et al. 2019

African Journal of Biological Sciences

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“…In hemp, previous studies on genetic diversity assessment have utilized RAPD markers (Faeti et al, 1996;Forapani et al, 2001;Mandolino and Ranalli, 2002). Microsatellite markers, in particular, have attracted interest as a tool to assess genetic diversity in a range of plant species, including those that are diecious (Barker et al, 2003;Teixeira et al, 2009;Dering et al, 2016;Szczecińska et al, 2016;Zhai et al, 2016;Kumar and Agrawal, 2019). Measures of genetic variability are expressed as the percent of polymorphic loci (P), number of alleles per locus (A), expected and observed heterozygosity (H E , H O ) and number of alleles per polymorphic loci (AP).…”

Section: Discussionmentioning

confidence: 99%

Hermaphroditism in Marijuana (Cannabis sativa L.) Inflorescences – Impact on Floral Morphology, Seed Formation, Progeny Sex Ratios, and Genetic Variation

Punja

Holmes

2020

Front. Plant Sci.

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Cannabis sativa L. (hemp, marijuana) produces male and female inflorescences on different plants (dioecious) and therefore the plants are obligatory out-crossers. In commercial production, marijuana plants are all genetically female; male plants are destroyed as seed formation reduces flower quality. Spontaneously occurring hermaphroditic inflorescences, in which pistillate flowers are accompanied by formation of anthers, leads to undesired seed formation; the mechanism for this is poorly understood. We studied hermaphroditism in several marijuana strains with three objectives: (i) to compare the morphological features of this unique phenotype with normal male flowers; (ii) to assess pollen and seed viability from hermaphroditic flowers; and (iii) to assess the effect of hermaphroditism on progeny male:female (sex) ratios and on genetic variation using molecular methods. The morphological features of anthers, pollen production and germination in hermaphroditic flowers and in staminate inflorescences on male plants were compared using light and scanning electron microscopy. Seeds produced on hermaphroditic plants and seeds derived from cross-fertilization were germinated and seedlings were compared for gender ratios using a PCR-based assay as well as for the extent of genetic variation using six ISSR primers. Nei's index of gene diversity and Shannon's Information index were compared for these two populations. The morphology of anthers and pollen formation in hermaphroditic inflorescences was similar to that in staminate flowers. Seedlings from hermaphroditic seeds, and anther tissues, showed a female genetic composition while seedlings derived from cross-fertilized seeds showed a 1:1 male:female sex expression ratio. Uniquely, hermaphroditic inflorescences produced seeds which gave rise only to genetically female plants. In PCR assays, a 540 bp size fragment was present in male and female plants, while a 390 bp band was uniquely associated with male plants. Sequence analysis of these fragments revealed the presence of Copia-like retrotransposons within the C. sativa genome which may be associated with the expression of male or female phenotype. In ISSR analysis, the percentage of polymorphic loci ranged from 44 to 72% in hermaphroditic and cross-fertilized

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“…Marker index (MI) is the primer capacity to detect polymorphic loci among different genotypes and was calculated as EMRxPIC. Resolving power (RP) is the ability of primers to distinguish between genotypes and was calculated as RP = ΣIb, where Ib is the informative fragments and can take values of: 1 − [2|0.5 − p|]; p is the proportion of total genotypes containing the band [47]. Genetic similarity was obtained according to the Jaccard similarity index.…”

Section: Molecular Analysismentioning

confidence: 99%

Genetic and Genomic Diversity in a Tarwi (Lupinus mutabilis Sweet) Germplasm Collection and Adaptability to Mediterranean Climate Conditions

et al. 2019

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Lupinus mutabilis (tarwi) is a species of Andean origin with high protein and oil content and regarded as a potential crop in Europe. The success in the introduction of this crop depends in part on in depth knowledge of the intra-specific genetic variability of the collections, enabling the establishment of breeding and conservation programs. In this study, we used morphological traits, Inter-Simple Sequence Repeat markers and genome size to assess genetic and genomic diversity of 23 tarwi accessions under Mediterranean conditions. Phenotypic analyses and yield component studies point out accession LM268 as that achieving the highest seed production, producing large seeds and efficiently using primary branches as an important component of total yield, similar to the L. albus cultivars used as controls. By contrast, accession JKI-L295 presents high yield concentrated on the main stem, suggesting a semi-determinate development pattern. Genetic and genomic analyses revealed important levels of diversity, however not relatable to phenotypic diversity, reflecting the recent domestication of this crop. This is the first study of genome size diversity within L. mutabilis, revealing an average size of 2.05 pg/2C (2001 Mbp) with 9.2% variation (1897, prompting further studies for the exploitation of this diversity.

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Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers

Cited by 39 publications

References 55 publications

Assessment of genetic diversity among some fennel cultivars (Foeniculum vulgare Mill.) by ISSR and SCoT Markers

Assessment of genetic diversity among some fennel cultivars (Foeniculum vulgare Mill.) by ISSR and SCoT Markers

Hermaphroditism in Marijuana (Cannabis sativa L.) Inflorescences – Impact on Floral Morphology, Seed Formation, Progeny Sex Ratios, and Genetic Variation

Genetic and Genomic Diversity in a Tarwi (Lupinus mutabilis Sweet) Germplasm Collection and Adaptability to Mediterranean Climate Conditions

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