Assessment of genetic diversity among some fennel cultivars (Foeniculum vulgare Mill.) by ISSR and SCoT Markers

Abstract

Fennel (Family Apiaceeae) is one of those strategic medicinal and aromatic plants in Egypt with great value as an economical exported plant. Fennel undergoes propagation through breeding of some fennel cultivars from different world region in order to keep out of genetic deterioration in the native Egyptian cultivars, and enrich its favorable active ingredients. The advanced plant breeding programs depend on some markers such as morphological, cytological, biochemical and molecular markers to study its genetic diversity. In this work two molecular markers ISSR and SCoT markers were used to assessment of genetic diversity among six fennel cultivars (Foeniculum vulgare Mill.) planted in Egypt; three of Egyptian origin (Balady, Elalamy and Azoricum) and the other ones are of different origin (Holland, Indian and German). The analyzed data reveals that the ISSR marker is more discriminating, provides more informative data than SCoT marker and can be reliable to evaluate the genetic diversity among the six examined cultivars as it performs the highest percentage of polymorphism 61% and higher unique bands number (4). According to ISSR molecular marker German and Indian cultivars are close distant; can exchange gene in between them smoothly despite the ultimate change in the environmental and weather conditions of their origin land. In conclusion, according to Nie , s and li , s coefficient based on SCoT marker analysis between the cultivars; Indian cultivar was quite distant from Azoricum by the same time that Azoricum is close distant to Holland, as well as; Elalamy is close distant to Balady.

Description and Evaluation of Some Newly Introduced Grape Cultivars Under Egyptian Conditions وصف وتقييم بعض أصناف العنب المستوردة حديثاً تحت الظروف المصرية

“…They found that the SCoT marker was more informative and effective than the ISSR marker to estimate the genetic diversity and perform fingerprinting in these plants. While this result disagrees with Ramadan et al (2019), who found that the ISSR marker is more discriminating and provides more informative data than SCoT in fennel cultivars.…”

Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques

“…They found that the SCoT marker was more informative and effective than the ISSR marker to estimate the genetic diversity and perform fingerprinting in these plants. While this result disagrees with Ramadan et al (2019), who found that the ISSR marker is more discriminating and provides more informative data than SCoT in fennel cultivars. While Baghizadeha and Dehghan (2018) and other researchers recommended that it is preferable to use these molecular marker techniques in combination with each other for distinctive fingerprinting.…”

Association between Productivity, Fatty Acid Profiles, Oil Bodies’ Ultrastructure and Molecular Markers in Peanut (Arachis hypogaea L.) Cultivars

“…With the primer RMN-P3, the PIC of RAPD was 0.37 and the minimum PIC value of 0.15 was recorded with the OP-B18 primer with ISSR; the highest PIC record was 0.30 for primers UBC-812 and UBC-814, while the lowest was 0.0 for the primer UBC-810, with an average of 0.25. Ramadan et al [17] found more discriminating results with the ISSR marker than the SCoT marker among fennel cultivars. RAPD was also found to be an effective tool in detecting genetic diversity in peanut cultivars [18].…”

“…Ismailia-1 contained large oil bodies with D50 of 6.7 µm. The number of existing oil bodies in a slide measurement indicated the difference in abundance among cultivars (17,59 and 63% in Ismailia-1, Samnut-26 and NC-7, respectively). Samnut-22 and Ex-Dakar had a higher abundance (82 and 80%, respectively) (Table 8).…”

“…Moreover, the markers are useful in genetic diversity estimation, phylogenetic studies, gene tagging, genome mapping and evolutionary biology in a wide range of plant species [9,16]. Ramadan et al [17] evaluated the genetic diversity of six Egyptian fennel cultivars using Inter Simple Sequence Repeat (ISSR) and Start Codon Targeted (SCoT) markers. They found more discriminating results with the ISSR marker because it had the highest percentage of polymorphisms compared to the SCoT marker.…”