Developing a dual-RCA microfluidic platform for sensitive E. coli O157:H7 whole-cell detections

Jiang, Yuqian; Qiu, Zhenyu; Le, Tao; Zou, Shan; Cao, Xudong

doi:10.1016/j.aca.2020.06.046

Cited by 40 publications

(36 citation statements)

References 49 publications

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“…Many significant devices and platforms have been proposed. The review has shown that microfluidic devices integrated with amplification components such as PCR [ 39 ], LAMP [ 45 ], HCR [ 47 ], CHA [ 48 ], and RCA [ 91 ] provide an effective means of performing the timely detection of foodborne pathogens, such as S. aureus, Streptococcus, E. coli, Listeria, Salmonella, V. vulnificus, and Bacillus anthracis in milk samples. Integrated rotating microfluidic systems with multiple functions such as DNA extraction and LAMP amplification have also been successfully applied for foodborne pathogen detection [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the problem of developing microfluidic platforms for the on-site detection of common foodborne pathogens in milk samples has attracted great interest in the recent literature. Typically, these platforms have been designed for the detection of E. coli [ 86 , 90 , 91 , 92 , 93 ], S. aureus [ 94 , 95 , 96 ], Listeria [ 97 , 98 , 99 ], and Salmonella [ 100 , 101 , 102 , 103 , 104 ].…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

“…However, in severe cases, it can cause more serious problems such as sepsis and hemolytic uremic syndrome [ 105 ]. Various microfluidic platforms have been developed for the detection of E. coli in milk samples, including lab-on-a-chip devices combined with immunoassay [ 78 ], aptamer and fluorescence detection [ 76 ], rolling circle amplification (RCA), and fluorescence detection [ 91 ], and lab-on-paper devices based on RCA and colorimetric detection [ 105 ], colorimetric detection [ 86 , 106 ], and immunoassay [ 90 , 92 , 93 ]. Sun et al [ 105 ] developed a folding microfluidic lab-on-paper RCA device with DNAzyme-containing DNA superstructures for E. coli detection in juice and milk samples.…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

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Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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Section: Discussionmentioning

confidence: 99%

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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show abstract

“…One of the possible, and still more and more popular, is the use of electrochemical aptasensors as detection elements in such. This is especially driven by the microelectronics progress and digitalization of the surrounded world, which led, e.g., to the production of miniaturized handheld point-of-care (PoC) devices [191][192][193][194][195]. Such a medical diagnostic development is of special interest as in an aging society, improvement of quality of life is one of the most important goals.…”

Section: Microfluidic Devices and Point-of-care Testingmentioning

confidence: 99%

“…In turn, this would significantly increase the awareness of the current health status of an individual person, and the same time, it would reduce the time necessary to implement appropriate medical procedures. Such devices, in comparison to traditional laboratory procedures, offer significantly reduced sample volume and shorter detection time, improved sensitivity due to high surface to volume ratio (also surface modified with carefully chosen nanomaterials), high throughput by parallel operation, portability, and disposability and what is no less important also real-time detection and an automated measurement process [191][192][193][194][195].…”

Section: Microfluidic Devices and Point-of-care Testingmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

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This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

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Biotechnological Advances in Detection of Contaminants from Wastewater

Mehta

Patel

Mukherjee

et al. 2022

CLEAN Soil Air Water

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Wastewater from industries causes major environmental pollution problems. The urban development and commercialization in developing countries cause deterioration of surface water resources. Hence, for its improvement, it is essential to develop highly sensitive devices with quick response for monitoring of wastewater contamination. In this respect, biotechnological processes such as biosensors can be promising and a robust option for detection of toxic pollutants. Biosensors can be applied in order to acquire real‐time quantitative data. Nowadays, the enzymes or whole cell bio‐reporters‐based biosensors have promising potential due to the possibility of miniaturization and opportunity for development of in situ biosensors. The growing need of convenient, rapid, and efficient biosensing devices explains the current advancement of biosensors with new transduction materials for multiplexed contaminant detection. Considering the extensive range of sensing strategies including immunochemical‐based, enzymatic and nonenzymatic signals, different kinds of biosensors can be classified as electrochemical, optical, thermal, etc. For the past few years, more attention has been paid to nanomaterial‐based biosensors which exhibit monitoring on the basis of chemical reactions and biological phenomena. Overall, this review focuses on principle and operation (transduction mechanism) of biosensors based on bio‐recognition elements, for the early detection of organic and inorganic contaminants existing in wastewater.

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Developing a dual-RCA microfluidic platform for sensitive E. coli O157:H7 whole-cell detections

Cited by 40 publications

References 49 publications

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Biotechnological Advances in Detection of Contaminants from Wastewater

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