Determining intestinal viability by near infrared spectroscopy: A veterinary application

Kohlenberg, Elicia; Payette, Jeri R.; Sowa, Michael G.; Levasseur, M; Riley, Christopher B.; Leonardi, Lorenzo

doi:10.1016/j.vibspec.2005.02.022

Cited by 8 publications

(9 citation statements)

References 10 publications

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“…Reference data (T0) are lacking as a result of abundant skin pigmentation in horses, which significantly alters light absorption and scattering, 39 making NIRS unusable on intact skin with no white markings. Moreover, specular reflectance created by highly reflective surfaces such as a fresh bleeding wound 27 handicapped data collection in wounds less than 24 hours old. Nonetheless, the study's main objective was to document differences between body and limb wounds.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue oxygen status (StO 2 ) can be evaluated using spectrophotometric techniques such as Near InfraRed Spectroscopy (NIRS) 24–27 . NIRS is a rapid, noninvasive analytical technique based on the relative ease with which near‐infrared light passes through biological tissues.…”

mentioning

confidence: 99%

“…Tissue oxygen status (StO 2 ) can be evaluated using spectrophotometric techniques such as Near InfraRed Spectroscopy (NIRS). [24][25][26][27] NIRS is a rapid, noninvasive analytical technique based on the relative ease with which near-infrared light passes through biological tissues. The concentrations of deoxyhemoglobin (dHb) and oxyhemoglobin (HbO 2 ) chromophores vary in relation to blood volume and oxygenation such that changes in the [dHb] and [HbO 2 ] in wound tissue provide information on tissue oxygenation status and blood volume.…”

mentioning

confidence: 99%

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Regional differences in wound oxygenation during normal healing in an equine model of cutaneous fibroproliferative disorder

Céleste

Deschêne

Riley

et al. 2010

Wound Repair Regeneration

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Wound repair in horse limbs is often complicated by the development of exuberant granulation tissue (EGT) and excessive scarring while body wounds tend to repair uneventfully. EGT resembles the human keloid. While the events leading to keloid formation are not fully elucidated, tissue hypoxia has been proposed as a major contributing factor. The objective of this study was to investigate tissue oxygen saturation in healing full-thickness wounds created on the horse limb and body, using near-infrared spectroscopy. Spectroscopic reflectance data were collected from both anatomic sites at specific times following wounding. The oxygen saturation values of limb wounds were significantly inferior to those of body wounds during the early period of healing, indicating a temporary, relative state of hypoxia in the former during the inflammatory phase of repair. Horses present a weak, persistent inflammatory response to wounding, especially at the limb level. The relative hypoxia present acutely in limb wounds of horses may promote a feeble yet prolonged inflammatory response, which could interfere with and retard the subsequent phases of healing. Ongoing low-grade inflammation in horse wounds is accompanied by up-regulation of various inflammatory and profibrotic mediators, which might ultimately promote the development of fibroproliferative disorders such as EGT.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Regional differences in wound oxygenation during normal healing in an equine model of cutaneous fibroproliferative disorder

Céleste

Deschêne

Riley

et al. 2010

Wound Repair Regeneration

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“…Focusing on other parameters: Intravital microscopy (Yasumura et al, 2003) Bowel wall contractility measurements (Dutkiewicz et al, 1997) Doppler ultrasound Microdialysis ) (flow, permeability and metabolic changes) Pulse oximetry (Erikoglu et al, 2005) Electrical bioimpedance (Carey et al, 1964) (electrical properties tied to structural changes) Hydrogen gas clearance (Mishima et al, 1979) Radioisotope studies (Prinzen and Bassingthwaighte, 2000) Fluorescence studies (Holmes et al, 1993, Boni et al, 2016, Johnson et al, 2016 Infrared imaging (Moss et al, 1978) Laser doppler flowmetry (LDF) (Humeau et al, 2007) Visible light spectrophotometry (VLS) (Karliczek et al, 2010) Near-infrared spectrophotometry (Kohlenberg et al, 2005) Diffuse reflectance spectroscopy (Karakas et al, 2014) pH measurement (Millan et al, 2006)) Of the methods listed in Table 1, visible light spectrophotometry (VLS) and laser doppler flowmetry (LDF) have been most frequently tested on humans (Urbanavicius et al, 2011). The sensor principle of VLS is transmission of visible light around 475-625 nm wavelengths, penetrating about 2 mm into the tissue.…”

Section: Focusing On Detection Of Blood Flowmentioning

confidence: 99%

In vivocharacterization of ischemic small intestine using bioimpedance measurements

et al. 2016

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The standard clinical method for the assessment of viability in ischemic small intestine is still visual inspection and palpation. This method is non-specific and unreliable, and requires a high level of clinical experience. Consequently, viable tissue might be removed, or irreversibly damaged tissue might be left in the body, which may both slow down patient recovery. Impedance spectroscopy has been used to measure changes in electrical parameters during ischemia in various tissues. The physical changes in the tissue at the cellular and structural levels after the onset of ischemia lead to time-variant changes in the electrical properties. We aimed to investigate the use of bioimpedance measurement to assess if the tissue is ischemic, and to assess the ischemic time duration. Measurements were performed on pigs (n = 7) using a novel two-electrode setup, with a Solartron 1260/1294 impedance gain-phase analyser. After induction of anaesthesia, an ischemic model with warm, full mesenteric arterial and venous occlusion on 30 cm of the jejunum was implemented. Electrodes were placed on the serosal surface of the ischemic jejunum, applying a constant voltage, and measuring the resulting electrical admittance. As a control, measurements were done on a fully perfused part of the jejunum in the same porcine model. The changes in tan δ (dielectric parameter), measured within a 6 h period of warm, full mesenteric occlusion ischemia in seven pigs, correlates with the onset and duration of ischemia. Tan δ measured in the ischemic part of the jejunum differed significantly from the control tissue, allowing us to determine if the tissue was ischemic or not (P < 0.0001, F = (1,75.13) 188.19). We also found that we could use tan δ to predict ischemic duration. This opens up the possibility of real-time monitoring and assessment of the presence and duration of small intestinal ischemia.

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“…In those works, a hyper spectral camera or a combination of LED light source and color CCD camera were used. Among those studies, H. Akbari et al [1] and E. Kohlenberg et al [2] segmented the ischemic site by focusing on the differences of the spectral reflectance between the ischemic intestine and normal intestine. Other groups [3]- [5] presented estimation methods of oxygen saturation based on reflected intensity analysis.…”

Section: Introductionmentioning

confidence: 99%

Fundamental Study on Intraoperative Quantification of Gastrointestinal Viability by Transmission Light Intensity Analysis

Yoshitaka

Ohnishi

Kawahira

et al. 2014

Lecture Notes in Computer Science

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Abstract. In gastrointestinal surgery, surgeon subjectively judges if the organ is healthy from the color. However it is difficult to discriminate a small difference of organ's color by visual inspection. In this paper, we focus on the tissue oxygen saturation (StO 2 ) that represents balance of oxygen demand and supply in tissue and try to estimate its value by transmitted light intensity analysis. We developed a system for measurement of transmitted light intensity using a compact spectrometer and a halogen light source and collected transmitted light intensity data from pig's small intestines. Absorbance of the tissue was then calculated from those data. On the basis of Beer-Lambert law, we estimated StO 2 from the calculated absorbance. Results of evaluation experiment to pig's small intestines suggested the possibility of quantitative evaluation of tissue viability by the proposed method.

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Determining intestinal viability by near infrared spectroscopy: A veterinary application

Cited by 8 publications

References 10 publications

Regional differences in wound oxygenation during normal healing in an equine model of cutaneous fibroproliferative disorder

Regional differences in wound oxygenation during normal healing in an equine model of cutaneous fibroproliferative disorder

In vivocharacterization of ischemic small intestine using bioimpedance measurements

Fundamental Study on Intraoperative Quantification of Gastrointestinal Viability by Transmission Light Intensity Analysis

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