Determination of α- and β-amanitin in clinical urine samples by Capillary Zone Electrophoresis

Amatoxin and phallotoxin concentration inAmanita phalloidesspores and tissues

“…If the transplantation cannot be done, the patients may die within 5-7 days (Ferreira et al, 2011). BA showed similar characteristics in limited number of studies (Robinson-Fuentes et al, 2008). Relatively little is known about other amatoxins, and there is a strong need for further studies.…”

Simultaneous analysis of α-amanitin, β-amanitin, and phalloidin in toxic mushrooms by liquid chromatography coupled to time-of-flight mass spectrometry

“…Regarding the toxicity of phalloidin, there is a report insisting that the compound does not exert poisonous effects by its oral intake [1]. However, after parenteral administration of The analysis of amanitin(s) and/or phalloidin has been made using high-performance liquid chromatography [4−6], capillary electrophoresis, capillary electrophoresis coupled to mass spectrometry (MS) [7,8], and liquid chromatography (LC) coupled to MS or to tandem MS [9−11]. Although the latter LC-MS (-MS) methods seem to give the most reliable and sensitive results, each of the three reported methods [9−11] has its drawbacks.…”

Fabrication of a biomimetic adsorbent imprinted with a common specificity determinant for the removal of α- and β-amanitin from plasma

“…This may be attributed to the carboxyl group of Asp residue in β-amanitin which does not exist in α-amanitin. Therefore, at pH 7.2, α-amanitin was neutral, whereas β-amanitin was acidic, so β-amanitin was negatively charged due to ionization [41]. The repelling interaction between partially ionized MAA and β-amanitin could interfere the recognition of β-amanitin and decrease the specific adsorption of β-amanitin on the biomimetic 16 adsorbent.…”