Determination of veterinary antibiotics in cow milk using rotating-disk sorptive extraction and liquid chromatography

Castillo-Aguirre, Alver; Cañas, Alejandro; Honda, Luis; Richter, Pablo

doi:10.1016/j.microc.2020.105851

Cited by 22 publications

(4 citation statements)

References 28 publications

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“…Although these advantages of ROX and other macrolides make them attractive in veterinary practice for use in the prevention and treatment of bacterial pathogens, abusive and unsafe use of macrolides antibiotics in farms can lead to the accumulation of macrolides and their residues in food products derived from animals, thereby causing allergic reactions or toxic effects on consumers 27 . Thus far, various analytical approaches for detecting antibiotics in animal-derived food products have been developed, such as rotating-disk sorptive extraction and liquid chromatography 28 , electrophoresis 29 , desorption corona beam ionization coupled mass spectrometry 30 , and microbiological assays 31 . Microbiology assays lack selectivity and sensitivity.…”

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confidence: 99%

Water-stable perovskite-loaded nanogels containing antioxidant property for highly sensitive and selective detection of roxithromycin in animal-derived food products

Han

Sharipov

Hwang

et al. 2022

Sci Rep

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Luminescent inorganic lead halide perovskite nanoparticles lack stability in aqueous solutions, limiting their application to optical sensors. Here, hybrid CsPbBr3-loaded MIP nanogels were developed with enhanced stability in aqueous media. Multifunctional MIP nanogels with antioxidant function and hydrophobic cavities were synthesized from HEMA derivatives in the presence of roxithromycin as a template. The CsPbBr3 nanoparticles were loaded into pre-synthesized MIP nanogels via in-situ synthesis with a size distribution of 200 nm. The developed CsPbBr3-nanogel exhibits excellent stability to air/moisture and enhanced stability toward an aqueous solvent. The developed CsPbBr3-loaded MIP nanogels showed a selective and sensitive detection of ROX with a limit of detection calculated to be 1.7 × 10–5 μg/mL (20.6 pM). The developed CsPbBr3-loaded MIP antioxidant-nanogels were evaluated on practical application for the quantitative determination of ROX antibiotic in animal-derived food products with excellent analytical performance. The detection of ROX in animal-derived food products showed good recovery results, making them an ideal candidate for sensing ROX.

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confidence: 99%

Water-stable perovskite-loaded nanogels containing antioxidant property for highly sensitive and selective detection of roxithromycin in animal-derived food products

Han

Sharipov

Hwang

et al. 2022

Sci Rep

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“…Debido a esta situación, son muchos los métodos que se han creado para detectar los diferentes antibióticos en la leche. Además, se buscan opciones alternas para las diferentes enfermedades y el uso responsable de los antibióticos cuando sea la única opción (20)(21)(22). También se ha encontrado no solo resistencia a antibióticos, siendo los antiparasitarios otros productos que también han generado resistencia y son eliminados en la leche, afectando la inocuidad de los alimentos (23).…”

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An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies

Zaatout

2022

Microbiological Research

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“…Additionally, its usage is forbidden during egg-laying. 10 Currently, the methods reported for detecting trimethoprim residues mainly included both large-scale instrument confirmation and rapid bioanalysis techniques, specially such as high-performance liquid chromatography (HPLC), 11 highperformance liquid chromatography−tandem mass spectrometry (HPLC-MS/MS), 12,13 and immunoassay methods. 14 Among them, immunoassays have further developed into a crucial and practical screening tool for rapidly detecting trimethoprim residues due to their low cost and ease of use.…”

Section: Introductionmentioning

confidence: 99%

Development of an Open Droplet Microchannel-Based Magnetosensor for Immunofluorometric Assay of Trimethoprim in Chicken and Pork Samples with a Wide Linear Range

Wang,

Zhang,

Zeng

et al. 2024

J. Agric. Food Chem.

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Trimethoprim (TMP), functioning as a synergistic antibacterial agent, is utilized in diagnosing and treating diseases affecting livestock and poultry. Human consumption of the medication indirectly may lead to its drug accumulation in the body and increase drug resistance due to its prolonged metabolic duration in livestock and poultry, presenting significant health hazards. Most reported immunoassay techniques, such as ELISA and immunochromatographic assay (ICA), find it challenging to achieve the dual advantages of high sensitivity, simplicity of operation, and a wide detection range. Consequently, an open droplet microchannelbased magnetosensor for immunofluorometric assay (OMM-IFA) of trimethoprim was created, featuring a gel imager to provide a signal output derived from the highly specific antibody (Ab) targeting trimethoprim. The method exhibited high sensitivity in chicken and pork samples, with LODs of 0.300 and 0.017 ng/mL, respectively, and a wide linear range, covering trimethoprim's total maximum residue limits (MRLs). Additionally, the spiked recoveries in chicken and pork specimens varied between 81.6% and 107.9%, maintaining an acceptable variation coefficient below 15%, aligning well with the findings from the ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technique. The developed method achieved a much wider linear range of about 5 orders of magnitude of 10 −2 −10 3 levels with grayscale signals as the output signal, which exhibited high sensitivity, excellent applicability and simple operability based on magnetic automation.

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Determination of veterinary antibiotics in cow milk using rotating-disk sorptive extraction and liquid chromatography

Cited by 22 publications

References 28 publications

Water-stable perovskite-loaded nanogels containing antioxidant property for highly sensitive and selective detection of roxithromycin in animal-derived food products

Water-stable perovskite-loaded nanogels containing antioxidant property for highly sensitive and selective detection of roxithromycin in animal-derived food products

An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies

Development of an Open Droplet Microchannel-Based Magnetosensor for Immunofluorometric Assay of Trimethoprim in Chicken and Pork Samples with a Wide Linear Range

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