Determination of Variations of Some Enzymes in Orchardgrass ( Dactylis glomerata L.) Ecotypes

Tosun, Metin; Akgün, İlknur; Taspinar, Mahmut Sinan; Kanli, Meryem

doi:10.1080/090647102321089855

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…Variations in cocksfoot morphological features, distributional patterns, adaptive, agronomic characters and allozymes have been well documented (Lumaret et al 1987;Sahuquillo and Lumaret 1995;Volaire and Thomas 1995;Gauthier et al 1998;Gauthier and Lumaret 1999;Tosun et al 2002). More recently, a number of molecular genetic marker systems have been developed for use in cocksfoot breeding, including random amplified polymorphic DNA (RAPD) markers (Kolliker et al 1999;Tuna et al 2004;, amplified fragment length polymorphism (AFLP) (Reeves et al 1998;Peng et al 2008), inter-simple sequence repeat (ISSR) markers ) and sequence-related amplified polymorphism (SRAP) markers (Zeng et al 2008).…”

Section: Mots Clé S: Dactylis Glomerata L Fragments Microsatellitaimentioning

confidence: 99%

Diversity comparison and phylogenetic relationships of cocksfoot (Dactylis glomerata L.) germplasm as revealed by SSR markers

Xie¹,

Zhang²,

Ma³

et al. 2010

Can. J. Plant Sci.

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. 2010. Diversity comparison and phylogenetic relationships of cocksfoot (Dactylis glomerata L.) germplasm as revealed by SSR markers. Can. J. Plant Sci. 90: 13Á21. Phylogenetic relationships among and within 16 accessions collected from China, Russia, Kazakhstan, India and Japan were investigated using SSR markers. The 21 SSR primer pairs generated a total of 143 polymorphic alleles, with an average of 6.8 alleles per locus, and a range of 3Á13 alleles. The average genetic diversity, as measured by the polymorphic information content (PIC), was 0.33. The average polymorphic rate (P) for this species was 90.7%, suggesting a high degree of genetic diversity. Analysis of molecular variation (AMOVA) revealed larger genetic variation within accessions (65.75%) and geographical regions (75.58%) rather than between them, while among ploidy level variance contributed only 2.62%. A high degree of genetic diversity within tetraploid group was detected based on the polymorphic rate (P 094.23%) and shannon's information index of diversity (I00.4300) when compared with diploid group (P 091.25%, I 00.3922). The values of the genetic identity (GI) among accessions ranged from 0.7320 to 0.9691 and were used to produce a dendrogram using the UPGMA approach. The 16 accessions were grouped into two major clusters (GI00.876); specifically, all tetraploid (2n 04x028) accessions originating from different regions were grouped into the same cluster, whereas the diploid (2n 02x014) accessions were grouped into the two major clusters. The dendrogram was concordant with the morphological variability, agronomic traits and karyotype. Generally, the results of this study indicate that cocksfoot germplasm contains high molecular variation within accession and geographical region. The implications of these results for conservation of the species were discussed. Les 21 paires d'amorces SSR ont donne´143 alle`les polymorphes, avec une moyenne de 6,8 alle`les par locus et une fourchette de 3 a`13 alle`les. La diversite´ge´ne´tique moyenne e´tablie d'apre`s l'importance de l'information polymorphe est de 0,33. Le taux moyen de polymorphisme pour l'espe`ce est de 90,7%, ce qui laisse croire a`une grande diversite´ge´ne´tique. L'analyse de la variation mole´culaire (AMOVA) re´ve`le que la variation ge´ne´tique est plus grande au sein des obtentions (65,75%) et a`l'inte´rieur des re´gions (75,58%) qu'entre elles, mais la variance des ploı¨dies n'explique que 2,62% de cette variation. Le taux de polymorphisme (P 094,23%) et l'indice Shannon de diversite´(I 00,4300) re´ve`lent une grande diversite´ge´ne´tique pour les groupes te´traploı¨des, comparativement aux groupes diploı¨des (P 091,25%, I 00,3922). L'identite´ge´ne´tique (GI) des obtentions varie de 0,7320 a`0,9691; on s'en est servi pour produire un dendrogramme par la me´thode UPGMA. Les 16 obtentions ont e´te´re´unies en deux grandes grappes (GI00,876), plus pre´cise´ment, les obtentions te´traploı¨des (2n04 x028) venant des diverses re´gions se sont toutes retrouve´es dans une grappe, alors ...

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Section: Mots Clé S: Dactylis Glomerata L Fragments Microsatellitaimentioning

confidence: 99%

Diversity comparison and phylogenetic relationships of cocksfoot (Dactylis glomerata L.) germplasm as revealed by SSR markers

Xie¹,

Zhang²,

Ma³

et al. 2010

Can. J. Plant Sci.

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“…Variations in orchardgrass morphological features, distributional patterns, adaptive and agronomic characters, and allozymes are well documented (Lumaret et al 1987; Lumaret and Borrientos 1990; Sahuquillo and Lumaret 1995; Volaire and Thomas 1995; Volaire 1995; Bretagnolle and Thompson 1996; Gauthier et al 1998; Gautier and Lumaret 1999; Lindner et al 1999; Sugiyama and Nakashima 1999; Tosun et al 2002). DNA profiling techniques that have been successfully used in assessing genetic diversity and relatedness of orchardgrass germplasm include randomly amplified polymorphic DNA (RAPD) markers (Kolliker et al 1999; Tuna et al 2004; Zeng et al 2006a) and inter‐simple sequence repeat (ISSR) (Zeng et al 2006b).…”

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Evaluation of genetic diversity in wild orchardgrass (Dactylis glomerata L.) based on AFLP markers

et al. 2008

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Studnicki M., Mądry W., Schmidt J. (2013): Comparing the efficiency of sampling strategies to establish a representative in the phenotypic-based genetic diversity core collection of orchardgrass (Dactylis glomerata L.). Czech J. Genet. Plant Breed., 49: 36-47. Establishing a core collection that represents the genetic diversity of the entire collection with a minimum loss of its original diversity and minimal redundancies is an important problem for gene bank curators and crop breeders. In this paper, we assess the representativeness of the original genetic diversity in core collections consisting of one-tenth of the entire collection obtained according to 23 sampling strategies. The study was performed using the Polish orchardgrass Dactylis glomerata L. germplasm collection as a model. The representativeness of the core collections was validated by the difference of means (MD%) and difference of mean squared Euclidean distance (d-D%) for the studied traits in the core subsets and the entire collection. In this way, we compared the efficiency of a simple random and 22 (20 cluster-based and 2 direct cluster-based) stratified sampling strategies. Each cluster-based stratified sampling strategy is a combination of 2 clusterings, 5 allocations and 2 methods of sampling in a group. We used the accession genotypic predicted values for 8 quantitative traits tested in field trials. A sampling strategy is considered more effective for establishing core collections if the means of the traits in a core are maintained at the same level as the means in the entire collection (i.e., the mean of MD% in the simulated samples is close to zero) and, simultaneously, when the overall variation in a core collection is greater than in the entire collection (i.e., the mean of d-D% in the simulated samples is greater than that obtained for the simple random sampling strategy). Both cluster analyses (unweighted pair group method with arithmetic mean UPGMA and Ward) were similarly useful in constructing those sampling strategies capable of establishing representative core collections. Among the allocation methods that are relatively most useful for constructing efficient samplings were proportional and D2 (including variation). Within the Ward clusters, the random sampling was better than the cluster-based sampling, but not within the UPGMA clusters.

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“…1994, Bennett and Leitch 1997). Because of its auto‐tetraploid status and large genome, the genetic markers that were reported for this species are mainly non‐species‐specific types such as isozymes (Tosun et al. 2002), randomly amplified polymorphic DNA (Kölliker et al.…”

Section: High‐scoring Matches Between the 969 Unique Primers And Thementioning

confidence: 99%

“…Orchardgrass has 28 chromosomes and is a mostly crosspollinated, auto-tetraploid species with a large genome (4312 Mbp) (Creber et al 1994, Bennett andLeitch 1997). Because of its auto-tetraploid status and large genome, the genetic markers that were reported for this species are mainly non-species-specific types such as isozymes (Tosun et al 2002), randomly amplified polymorphic DNA (Ko¨lliker et al 1999, Tuna et al 2004, amplified fragment length polymorphisms (AFLP) (Peng et al , 2008, inter-simple sequence repeats (ISSRs) (Zeng et al 2006) and sequence-related amplified polymorphisms (Zeng et al 2008).…”

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confidence: 99%

Isolation and characterization of simple sequence repeat markers for the tetraploid forage grass Dactylis glomerata

Hirata¹,

Yuyama²,

Cai

2011

Plant Breeding

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To isolate simple sequence repeat (SSR) markers from Dactylis glomerata, four SSR-enriched genomic libraries for different repeat motifs from genomic DNA of a Japanese orchardgrass variety, ÔAkimidori IIÕ, were constructed. A total of 969 unique SSR-containing clones were identified and used for primer design. A BLAST search revealed that 63 of the sequences showed significant homology with sequences of other plant species including 18 that showed significant gene homology. After screening of a panel consisting of eight orchardgrass individuals, 606 SSR markers showed polymorphism, 70 generated monomorphic bands and the other primers either generated multiple bands or did not provide any amplification product. In the 606 polymorphic SSR markers, most had a large number of alleles, ranging from 1 to 13 with an average of 4.5 alleles per SSR primer pair. The number of alleles per individual ranged from 1 to 4. These SSR markers developed in this study will have broad utility for genotyping applications in this species, including gene targeting, quantitative trait locus mapping, variety or species identification and marker-assisted selection.

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Determination of Variations of Some Enzymes in Orchardgrass ( Dactylis glomerata L.) Ecotypes

Cited by 3 publications

References 10 publications

Diversity comparison and phylogenetic relationships of cocksfoot (Dactylis glomerata L.) germplasm as revealed by SSR markers

Diversity comparison and phylogenetic relationships of cocksfoot (Dactylis glomerata L.) germplasm as revealed by SSR markers

Evaluation of genetic diversity in wild orchardgrass (Dactylis glomerata L.) based on AFLP markers

Isolation and characterization of simple sequence repeat markers for the tetraploid forage grass Dactylis glomerata

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