Determination of urinary N-acetyl-β-D glucosaminidase (NAG) levels in experimental blunt renal trauma

Hanbeyoğlu, Alpaslan; Kazez, Ahmet; Üstündağ, Bilal; Akpolat, Nusret

doi:10.5505/tjtes.2011.57973

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…In a wide spectrum of studies, researchers have evaluated the usefulness of measurements of serum and urine enzymes in the detection of kidney diseases [7-10, 12]. Serum and urine NGAL, KIM-1, IL-8, and Cys-C tests have been shown to be important in the detection of renal damage that develops secondary to ischaemia or other diseases and in monitoring these diseases [14-16].…”

Section: Discussionmentioning

confidence: 99%

“…These include urinary alkaline phosphatase, gamma glutamyl transferase, leucine aminopeptidase, alanine aminopeptidase, β-galactosidase, neutral endopeptidase, α-glucosidase, and N-acetyl-β-D glucosaminidase measurements. However, the poor stability of these molecules and difficulties in collecting urine for 24 h restrict the use of most of these indicators [12, 31-34]. NGAL, KIM-1, and IL-8 have potential as biochemical parameters in the determination of renal damage.…”

Section: Discussionmentioning

confidence: 99%

“…The rats were placed in metabolic cages containing standard pellets and tap water and monitored. Trauma group: the renal trauma model and apparatus were performed according to described by Hanbeyoglu et al [12]. Under general anaesthesia, through a midline incision, the abdominal cavity was entered.…”

Section: Methodsmentioning

confidence: 99%

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A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Bakal¹,

Saraç²,

Tartar³

et al. 2019

cejoi

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This study investigated changes in the concentrations of serum and urine neutrophil gelatinase lipocalin (NGAL), kidney injury molecule 1 (KIM-1), interleukin 18 (IL-18), and cystatin-C (Cys-C) induced by parenchymal and tubular damage following blunt kidney trauma, as well as their potential utility as biomarkers in the detection and follow-up of patients with suspected blunt renal trauma. Three-month-old male Sprague-Dawley rats (n = 18) were divided into three groups (n = 6 in each): group 1: control group (no intervention); group 2: sham group (explorative surgery and exposure of the left kidneys); and group 3: trauma group (explorative surgery and induction of blunt renal trauma of the left kidneys). Serum and urine samples were collected before and 12-24, 36-48, and 60-72 hours later for NGAL, KIM-1, IL-18, and Cys-C measurements. In the trauma group, there was a statistically significant increase in post-operative NGAL, KIM-1, and IL-18 values after 12-24 h and 36-48 h, as compared with pre-operative values. There was also a statistically significant increase in post-operative serum and urine Cys-C values after 60-72 h, as compared with pre-operative values. NGAL, KIM-1, and IL-18 may represent novel non-invasive descriptive candidate biomarkers of early-stage tubular damage in children with renal trauma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Bakal¹,

Saraç²,

Tartar³

et al. 2019

cejoi

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“…This is probably caused by abnormal metabolism and GRP excretion when kidney function is impaired. The kidney is the main organ excreting proGRP, a low molecular protein which could pass freely through the glomerular basement membrane . Therefore, in the early stage of renal injury, serum proGRP levels are mainly affected by electrolyte imbalance and gastrointestinal secretion.…”

Section: Discussionmentioning

confidence: 99%

“…The kidney is the main organ excreting proGRP, a low molecular protein which could pass freely through the glomerular basement membrane. 21 Therefore, in the early stage of renal injury, serum proGRP levels are mainly affected by electrolyte imbalance and gastrointestinal secretion. When renal failure progresses to the middle and late stages, the glomerular basement membrane is severely damaged, and proGRP could not pass through endothelial cells and the basilar membrane of the kidney, accumulating in blood circulation.…”

Section: Discussionmentioning

confidence: 99%

Expression and clinical value of gastrin‐releasing peptide precursor in nephropathy and chronic kidney disease

et al. 2019

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Aim To explore whether serum pro‐gastric releasing peptide (proGRP) is elevated in nephropathy patients and evaluate the diagnostic value of proGRP in chronic kidney disease (CKD) patients. Methods A total of 498 nephropathy patients and 170 healthy were selected in Zhongshan Hospital, Medical College of Xiamen University, from February 2016 to September 2017. The clinical data of the different groups including serum proGRP, CKD grading, and other serum and urine renal function biomarkers were analyzed by group comparison, correlation analysis and receiver operating characteristic curve. Results Serum proGRP levels were significantly higher in the acute kidney injury and CKD groups compared with the other groups of kidney disease patients (P < 0.01), and increased with CKD grading (P < 0.01). Serum proGRP was substantially correlated with serum creatinine (r = 0.637, P < 0.01) and cystain C (0.837, P < 0.01). Serum proGRP had moderate correlations with urine β2‐macroglobulin (β2‐m; r = 0.587, P < 0.01) and α1‐macroglobulin (α1‐m; r = 0.557, P < 0.01). There were fair associations of serum proGRP with albumin (r = 0.10, P = 0.067), 24 h proteinuria (24 h‐TPU; r = 0.092, P = 0.099), urinary albumin/urocreatinine (uAlb/Cr; r = 0.29, P < 0.01) and urinary N‐acetyl‐β‐D‐glucosidase (r = −0.142, P < 0.01). The sensitivity of proGRP was superior to that of simplified modification of diet in renal disease (MDRD) formula in diagnosing CKD I + II (81.25% vs 66.67%), CKD III (86.42% vs 74.36%) and CKD IV (71.19% vs 69.64%), while its specificity was inferior to that of simplified MDRD formula in diagnosing CKD I + II (37.65% vs 66.97%), CKD III (56.25% vs 86.67%) and CKD IV (75.31% vs 88.46%). Conclusion Serum proGRP is elevated in acute renal injury and CKD patients and increases with CKD grading. Serum proGRP is mainly affected by glomerular filtration rate and could be used for CKD staging, although the overall diagnostic sufficiency is inferior to simplified MDRD formula.

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A new biomarker (RENALASE) for the diagnosis of blunt renal trauma in an experimental study

Saraç

Bakal

Tartar

et al. 2021

Journal of Pediatric Urology

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Determination of urinary N-acetyl-β-D glucosaminidase (NAG) levels in experimental blunt renal trauma

Cited by 7 publications

References 20 publications

A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Expression and clinical value of gastrin‐releasing peptide precursor in nephropathy and chronic kidney disease

A new biomarker (RENALASE) for the diagnosis of blunt renal trauma in an experimental study

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