Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Tavlarakis, Panagiotis; Urban, John J; Snow, Nicholas H.

doi:10.1093/chrsci/49.6.457

Cited by 26 publications

(13 citation statements)

References 23 publications

(23 reference statements)

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“…Protein concentration was assayed using dye binding with bicinchoninic acid (BCA Protein Assay Kit, Pierce, Rockford, IL). PVP concentrations were measured using absorbance at 220 nm relative to a standard curve (24).…”

Section: Tetrahydrofuran Hydrate Bindingmentioning

confidence: 99%

Structural Basis for the Inhibition of Gas Hydrates by α -Helical Antifreeze Proteins

Sun

Davies

Walker

2015

Biophysical Journal

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Kinetic hydrate inhibitors (KHIs) are used commercially to inhibit gas hydrate formation and growth in pipelines. However, improvement of these polymers has been constrained by the lack of verified molecular models. Since antifreeze proteins (AFPs) act as KHIs, we have used their solved x-ray crystallographic structures in molecular modeling to explore gas hydrate inhibition. The internal clathrate water network of the fish AFP Maxi, which extends to the protein's outer surface, is remarkably similar to the {100} planes of structure type II (sII) gas hydrate. The crystal structure of this water web has facilitated the construction of in silico models for Maxi and type I AFP binding to sII hydrates. Here, we have substantiated our models with experimental evidence of Maxi binding to the tetrahydrofuran sII model hydrate. Both in silico and experimental evidence support the absorbance-inhibition mechanism proposed for KHI binding to gas hydrates. Based on the Maxi crystal structure we suggest that the inhibitor adsorbs to the gas hydrate lattice through the same anchored clathrate water mechanism used to bind ice. These results will facilitate the rational design of a next generation of effective green KHIs for the petroleum industry to ensure safe and efficient hydrocarbon flow.

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Section: Tetrahydrofuran Hydrate Bindingmentioning

confidence: 99%

Structural Basis for the Inhibition of Gas Hydrates by α -Helical Antifreeze Proteins

Sun

Davies

Walker

2015

Biophysical Journal

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“…To the best of our knowledge, the proposed sensors were the first sensors fabricated for the determination of the studied drugs. The previous work performed on these drugs was tedious, time consuming and requiring drug pre‐treatment or separation steps [8, 9, 18, 10–17, 25–27, 20, 21] while the proposed sensors are of great analytical value, as they offer fast response, simple and rapid analysis, and eliminate any drug pre‐treatment or separation steps. The incorporation of CH nanoparticles in the manufacture of PVP sensor readily improved the analytical response, the linear range and reduced the electrical resistance [32].…”

Section: Resultsmentioning

confidence: 99%

“…Polyvinylpyrrolidone (PVP) is a chain synthetic polymer of 1-vinyl-2-pyrrolidone [20] (Figure S1.b). When 3.5 % isotonic PVP was taken as plasma substitute, 40-60 % of it was excreted in urine unchanged mainly during the first two or three days after infusion [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Application of Precipitation‐based and Nanoparticle‐based Techniques for Fabrication of Potentiometric Sensors for Nano Molar Determination of Chitosan and Polyvinyl Pyrrolidone in Pharmaceutical Formulations and Biological Fluids

et al. 2021

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Chitosan (CH) is one of the most abundant biopolymers with multiple applications. Polyvinyl pyrrolidone (PVP) has specific binding and detoxification properties that are of great interest in health care. Hence, it arises a crucial urge to develop economic sensors to analyze CH and PVP in pharmaceutical formulations and biological samples. Two portable sensors were fabricated using precipitation‐based technique, and nanoparticles‐based technique, for determination of CH and PVP in sensor 1 and 2; respectively. Linear responses of 10−5 to10−7 M and 10−2 to10−7 M at pH 3.6–4.8 and 7.2–8.4, with ideal Nernstian slopes of 60.00 and 59.83 mV /decade, and nanomolar LODs of 94.90 and 81.20 nM were observed for CH and PVP; respectively. The percentage recoveries were 100.40±1.03 and 100.19±0.64 for sensors 1 and 2; respectively. Both sensors were successfully applied in biological fluids without pre‐treatment. Accurate results were obtained using sensor 1, in pure form, pharmaceutical formulations, human plasma, rat liver and rat brain, as well as sensor 2, in pure form, pharmaceutical formulations and urine samples. The results were statistically compared with the reported methods and no significant difference was observed.

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“…It is an odorless and non-flammable material [ 37 ]. Moreover, pVP exhibits hygroscopic and adhesive properties [ 38 , 39 ]. Due to rising ecological awareness, absolutely safe materials should be selected for the pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

2021

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Due to the unwanted toxic properties of some drugs, new efficient methods of protection of the organisms against that toxicity are required. New materials are synthesized to effectively disseminate the active substance without affecting the healthy cells. Thus far, a number of polymers have been applied to build novel drug delivery systems. One of interesting polymers for this purpose is povidone, pVP. Contrary to other polymeric materials, the synthesis of povidone nanoparticles can take place under various condition, due to good solubility of this polymer in several organic and inorganic solvents. Moreover, povidone is known as nontoxic, non-carcinogenic, and temperature-insensitive substance. Its flexible design and the presence of various functional groups allow connection with the hydrophobic and hydrophilic drugs. It is worth noting, that pVP is regarded as an ecofriendly substance. Despite wide application of pVP in medicine, it was not often selected for the production of drug carriers. This review article is focused on recent reports on the role povidone can play in micro- and nano drug delivery systems. Advantages and possible threats resulting from the use of povidone are indicated. Moreover, popular biomedical aspects are discussed.

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Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection

Cited by 26 publications

References 23 publications

Structural Basis for the Inhibition of Gas Hydrates by α -Helical Antifreeze Proteins

Structural Basis for the Inhibition of Gas Hydrates by α -Helical Antifreeze Proteins

Application of Precipitation‐based and Nanoparticle‐based Techniques for Fabrication of Potentiometric Sensors for Nano Molar Determination of Chitosan and Polyvinyl Pyrrolidone in Pharmaceutical Formulations and Biological Fluids

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

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