Determination of Total Lipid in the Cerebrospinal Fluid by Paper Chromatography

Determination of Fatty Acids in the Cerebrospinal Fluid IV. The Fatty Acid Content of the Major Lipid Fractions and the Total Fatty Acid Esters of Cerebrospinal Fluid

“…The mean total lipid content in the cerebro- spinal fluid is higher than found by other investigators (Tourtellotte et al 1962, Curtis & Seipel 1961. In cerebrospinal fluid from healthy persons, Tourtellotte et al (1962) found a mean lipid content of 1.25 mg/100 ml (0.766-1.738) by bichromate oxidation.…”

“…Cholesterol was not determined, but the calculated esterified cholesterol was The mean fatty acid content in the lipids of cerebrospinal fluid in this investigation is in agreement with previous findings in our laboratory (Farstad 1964 b) . The great relative standard deviation in the different fractions of cerebrospinal fluid lipids after silicic acid chromatography may reflect the problems mentioned by Curtis & Seipel (1961), and is probably not due to the fatty acid determination.…”

“…The discrepancies are partly due to differences in lipid extraction and methods of lipid determinaton, and partly to the origin of the cerebrospinal fluid samples. Varying amounts of the different fractions of the cerebrospinal fluid lipids also have been found (Brown, Gildea & Man 1938, Tourtellotte, Vander, Skrentny & De Jong 1958, Roboz, Hess, Di Nella & Cevallos 1958, Papadoupolos, Cevallos & Hess 1959and 1960, Curtis & Seipel 1961, Farstad 1964.…”

The Composition of Lipids in Cerebrospinal Fluid of Children and Adults

“…The fatty acid composition of these lipid classes for CSF derived from children was similar to that from adult subjects. Degradation of CSF lecithin by snake-venom phospholipase Az revealed the saturated acids to be located predominantly in the I-position with the unsaturated ones mainly in the 2-position.ALTHOUGH several studies have been conducted on both the lipid content (TOURTEL- LOTTE, 1959;CURTIS and SIEPEL, 1960; FARSTAD, 1965) and fatty acid composition (TUNA, LOGOTHETIS and KAMMERECK, 1963; FARSTAD, 1964) of adult human CSF, no corresponding information is available concerning the CSF of children. Considerable variations in the total lipid content of adult CSF has also been noted and been largely attributed to the different extraction procedures employed.…”

“…ALTHOUGH several studies have been conducted on both the lipid content (TOURTEL- LOTTE, 1959;CURTIS and SIEPEL, 1960; FARSTAD, 1965) and fatty acid composition (TUNA, LOGOTHETIS and KAMMERECK, 1963; FARSTAD, 1964) of adult human CSF, no corresponding information is available concerning the CSF of children. Considerable variations in the total lipid content of adult CSF has also been noted and been largely attributed to the different extraction procedures employed.…”