Determination of thiamine by high-performance liquid chromatography

Lynch, P L Mark

doi:10.1016/s0021-9673(00)00089-3

Cited by 117 publications

(71 citation statements)

References 121 publications

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“…TMP and free thiamine were the only detectable species in the extracellular fluid. The majority of TPP in the body is found in erythrocytes and accounts for approximately 80% of the total bodies stores [14,[42][43][44]. There is no consensus on reference intervals for thiamine compounds in the human body.…”

Section: Physiology Of Thiaminementioning

confidence: 99%

“…However, the upper limit of normal has been greatly debated with published values ranging from 15.5% to 40% of increased activity [3,115]. Further considerations are required when interpreting results from these tests as they are known to be affected by patients with diabetes or liver dysfunction as well as any other condition impeding the synthesis of transketolase [14,115]. Recent end-of-cycle reports from the RCPAQAP and SKML EQA vitamin programmes indicate that no participating laboratory is currently reporting TPP values using indirect methodology [120,121].…”

Section: Testing Procedures Considerationsmentioning

confidence: 99%

“…A greater understanding of human thiamine biochemistry is highly desirable and clinically relevant as thiamine deficiency (TD) is still reported worldwide. Despite long-term thiamine fortification of a variety of common household foods (meats, vegetables, milk products, cereals and whole grains), TD continues to be present even in modern, western societies [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Vitamin B1 in critically ill patients: needs and challenges

Collie

Greaves

Jones

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: Thiamine has a crucial role in energy production, and consequently thiamine deficiency (TD) has been associated with cardiac failure, neurological disorders, oxidative stress (lactic acidosis and sepsis) and refeeding syndrome (RFS). This review aims to explore analytical methodologies of thiamine compound quantification and highlight similarities, variances and limitations of current techniques and how they may be relevant to patients. Content: An electronic search of Medline, PubMed and Embase databases for original articles published in peerreviewed journals was conducted. MethodsNow was used to search for published analytical methods of thiamine compounds. Keywords for all databases included "thiamine and its phosphate esters", "thiamine methodology" and terms related to critical illness. Enquiries were also made to six external quality assurance (EQA) programme organisations for the inclusion of thiamine measurement.

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Section: Physiology Of Thiaminementioning

confidence: 99%

Section: Testing Procedures Considerationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vitamin B1 in critically ill patients: needs and challenges

Collie

Greaves

Jones

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The choice of the method of analysis depends on the accuracy and sensitivity required. In addition, intrinsic differences between the various types of food matrices and the presence of interfering compounds imply the need for the optimization of specific techniques of extraction and analysis [8,9].…”

Section: Introductionmentioning

confidence: 99%

Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Silveira

Lúcia

Pirozi

et al. 2016

Acta Chromatographica

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Summary. This study aimed to optimize and validate methods for the analysis of thiamin and folic acid in fortified rice, pure and mixed to the milled rice (raw and cooked). The analysis was performed by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Different mobile phases were tested. Different ratios of organic modifier, pH ranges, triethylamine concentrations, and flow rates were used. For the validation, tests of recovery, repeatability, linearity, limit of detection (LOD), and limit of quantification (LOQ) were performed. The optimized methods showed good resolution of vitamins' peaks, excellent recovery (82.6 to 104%), repeatability with relative standard deviation of peak areas, and retention times less than 10% and high coefficients of determination (0.9998 for thiamin and 0.9997 for folic acid). The LOD and LOQ were 0.00193 μg and 0.0193 μg for thiamin and 0.000934 μg and 0.00934 μg for folic acid. The optimized methods demonstrated reliability and sensitivity in the detection and quantification of these vitamins in fortified rice, pure and mixed to milled rice (raw and cooked). Furthermore, the methods were performed in isocratic mode, with short run time (<13 min), reflecting positively on the economy of reagents and analysis times.

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“…From an analytical point of view, these physicochemical properties make it difficult to analyze thiamine. 8 First, extremely polar thiamine is scarcely retained under general reverse phase conditions. Second, due to its amine structure, thiamine can strongly interact with free silanol groups on the surface of the stationary phase, which causes band broadening or tailing.…”

mentioning

confidence: 99%

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

Suh¹,

Kim²,

Jung³

et al. 2013

Bulletin of the Korean Chemical Society

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A novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs) was employed in the absence of volatile organic solvents or ion-pairing reagents to analyze thiamine, a very polar compound, by reverse phase high performance liquid chromatography (RP-HPLC). Due to its strongly hydrophilic nature, thiamine was eluted near the column dead time (t 0 ) using a mobile phase without adding RTILs or ion-pairing reagents, even if a 100% aqueous mobile phase, which has weak elution power under reverse phase conditions, was used. Thus, 1-ethyl-3-methyl-imidazolium hexafluorophosphate ([EMIM][PF 6 ]), which has the strongest chaotropic effect, was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t 0 . Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine thiamine at the proper retention time. Method validation was performed to assess linearity, intra-and inter-day accuracy and precision, recovery and repeatability; all results were found to be satisfactory. The developed method was also compared to the current official United States Pharmacopoeia (USP) and Korean Pharmacopoeia (KP) methods using an organic mobile phase containing an ionpairing reagent by means of evaluating various chromatographic parameters such as the capacity factor, theoretical plate number, peak asymmetry and tailing factor. The results indicated that the proposed method exhibited better efficiency of thiamine analysis than the official methods, and it was successfully applied to quantify thiamine in pharmaceutical preparations.

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Determination of thiamine by high-performance liquid chromatography

Cited by 117 publications

References 121 publications

Vitamin B1 in critically ill patients: needs and challenges

Vitamin B1 in critically ill patients: needs and challenges

Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

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