Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

Abstract

A novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs) was employed in the absence of volatile organic solvents or ion-pairing reagents to analyze thiamine, a very polar compound, by reverse phase high performance liquid chromatography (RP-HPLC). Due to its strongly hydrophilic nature, thiamine was eluted near the column dead time (t 0 ) using a mobile phase without adding RTILs or ion-pairing reagents, even if a 100% aqueous mobile phase, which has weak elution power under reverse phase conditions, was used. Thus, 1-ethyl-3-methyl-imidazolium hexafluorophosphate ([EMIM][PF 6 ]), which has the strongest chaotropic effect, was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t 0 . Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine thiamine at the proper retention time. Method validation was performed to assess linearity, intra-and inter-day accuracy and precision, recovery and repeatability; all results were found to be satisfactory. The developed method was also compared to the current official United States Pharmacopoeia (USP) and Korean Pharmacopoeia (KP) methods using an organic mobile phase containing an ionpairing reagent by means of evaluating various chromatographic parameters such as the capacity factor, theoretical plate number, peak asymmetry and tailing factor. The results indicated that the proposed method exhibited better efficiency of thiamine analysis than the official methods, and it was successfully applied to quantify thiamine in pharmaceutical preparations.

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

“…Some applications of ILs as mobile phase additives in RP-HPLC have been reported for the analysis of pharmaceutical compounds [ 125 , 126 , 127 , 128 , 129 , 130 ]. Different pharmaceutical compounds were analyzed such as β-blockers [ 125 ], antidepressants [ 126 ], β-lactam antibiotics [ 127 ], thiamine (vitamin B1) [ 128 ], urazamide [ 129 ], and ephedrines [ 130 ]. Among these, Ruiz-Angel et al [ 125 ] carried out a comparative study of the effect of 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]) and triethylamine (TEA) added to the mobile phase on the analysis of seven β-blockers in RP-HPLC.…”

Detection of vitamin b1 (thiamine) using modified carbon paste electrodes with polypyrrole

“…Vitamin B 1 is mixed with pyrrole monomer before electropolymerysation process that vitamin B 1 is at polipirola matrix. Trapped vitamin B 1 in polipirola occur through hydrogen interaction between the N-H group at the pyrrole with O-H and N-H group in vitamin B 1 . Following that, vitamin B 1 is extracted with a porogenic solvent in order to obtain moleculary imprinted polymer (MIP) on the surface of CPE.…”

“…Vitamin B 1 or thiamine (3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2hydroxyethyl)-4_methylthiazol-3-ium chloride) (figure 1) have a role in the development of brain and neurons [1]. There are at least 30 g Stock of vitamin B 1 in human body [2].…”

“…The importance of vitamin B 1 roles in human body, lead to development of methods to detect vit B 1 in foods or certain suplements. Some of the developed methods including high performance liquid chromatography [1], chemiluminesence [3], dan fluoresence [4]. The eminence of those methods are high accuracy and coefficient of determination, also low detection limit.…”

A novel online coupling of ion selective electrode with the flow injection system for the determination of vitamin B1

“…beriberi [2], and epilepsy [3]. Many of analytical methods are available for the determination of vitamin B 1 either alone or in conjunction with various drugs in pharmaceutical formulations and biological fluids using calorimetric [4], spectrophotometry [5][6][7], electrochemistry [8], fluorimetric [9], chromatography [10][11][12][13][14][15], chemiluminescence [16]. Potentiometric methods are more productive in innovations in view of the more possibilities for the design of the electrodes that can easily be converted into flow-through electrodes via passed in the flow cell.…”