Determination of theophylline in plasma by electron capture gas chromatography

Schwertner, Harvey A.; Ludden, Thomas M.; Wallace, Jack E.

doi:10.1021/ac50007a016

Cited by 29 publications

(6 citation statements)

References 11 publications

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“…51 paper was carried out with H,O-pyridine-AcOH (95: 1 : 10, pH 3.6) for 40min at 2450V/60cm, and the spots were visualized with Pauly reagent sprays. TRH and Oic-His-Pro-NH, (2a) were prepared as previously described (respectively 13,14). tert.-bury1 ( S ) -1-substituted 3-benzyloxycarbonyl-2-oxoimidazolidine-4-carboxylates (3b-e, g-k) and…”

Section: Experl M Ental Proceduresmentioning

confidence: 99%

Synthesis and central nervous system actions of thyrotropin‐releasing hormone analogs containing a 1‐substituted 2‐oxoimadazolidine moiety*

Maéda

Suzuki

Sugano

et al. 1989

International Journal of Peptide and Protein Research

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A series of thyrotropin-releasing hormone (TRH) analogs in which the pyroglutamic acid residue was replaced by (S)-2-oxoimidazolidine-4-carboxylic acid (Oic-OH) and the related derivatives was prepared, and the central nervous system (CNS) actions were examined. Of these, 1-benzyl-Oic-His-Pro-NH, (2c) showed the most potent activities, which were 1.5-8 times greater than those of TRH. Moreover, the thyrotropin (TSH)-releasing activity of 2c was about 1/16 times weaker than that of TRH.Key words: central nervous system; effect on pentobarbital anesthesia; effect on reserpine-induced hypothermia; (S)-2oxoimidazolidine-4-carboxylic acid; spontaneous locomotor activity; TRH analogs

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Section: Experl M Ental Proceduresmentioning

confidence: 99%

Synthesis and central nervous system actions of thyrotropin‐releasing hormone analogs containing a 1‐substituted 2‐oxoimadazolidine moiety*

Maéda

Suzuki

Sugano

et al. 1989

International Journal of Peptide and Protein Research

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“…One /A was directly injected into the gas chromatograph. For theophylline, according to SCHWF.RaaqER et al [7], 1 ml of plasma, 100 /tl of internal standard (3-isobutyl-l-methylxanthine) and 800 mg ammonium sulfate were extracted by 10 ml of a mixture of toluene and ethylacetate (8:2 v/v). The phases were separated by eentrifugation and the organic phase was evaporated to dryness.…”

Section: Pharmacoklnetle Determinationsmentioning

confidence: 99%

Anti-inflammatory effect of LA 2851 and reference drugs on some models of inflammation. Investigation of the mechanism of action

Junien¹,

Lakatos²,

Brohon³

et al. 1982

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LA 2851 (2-4-diamino-7-methyl-pyrazolo (1,5-a) 1,3,5-triazine), a bronchodilator and antiallergic compound, in type I hypersensitivity, has been tested orally for activity against carrageenan oedema and complement dependent, reverse passive Arthus (RPA) and zymosan oedema in rats. Pharmacokinetic determinations were also realized in order to correlate plasma blood levels and pharmacological activity. LA 2851 was found active in the first test but showed a more marked effect in the immunologically mediated RPA reaction and zymosan oedema. Among reference drugs tested, theophylline showed the same pattern in contrast with non-steroidal anti-inflammatory agents. LA 2851 and theophylline, using a superfused lung preparation, were found ineffective on the synthesis of cyclooxygenase products from arachidonic acid. LA 2851 as theophylline inhibited cAMP phosphodiesterase (PDE) but this inhibition does not seem to be involved in their anti-inflammatory activity since papaverine, a potent inhibitor of PDE, was totally inactive. The activity on RPA and zymosan inflammation was achieved at the drug plasma level in the range of those required to relax the trachea. The same antagonism was obtained with the 2 drugs at a lower plasma level with LA 2851 than with theophylline for the same dose administered (25 mg/kg). LA 2851 and theophylline did not inhibit all the components of the inflammatory process since maximum inhibition did not exceed 60% up to 200 mg/kg and 100 mg/kg respectively.

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“…Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever methods, namely ultraviolet spectroscopy ( 6 , 7 ) , gas liquid chromatography (8,9) and high pressure liquid chromatography (10) have been described to monitor serum theophylline levels.…”

Section: Specific Antibodies To Theophylline For Use In a Homogeneousmentioning

confidence: 99%

Specific Antibodies to Theophylline for Use in a Homogeneous: Enzyme Immunoassay

Singh

Gushaw

et al. 1980

Journal of Immunoassay

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Bovine serum albumin conjugate of 1-methyl-3-(3'-carboxypropyl)xanthine elicits highly specific anti-theophylline antibodies when injected into sheep. When used in a homogeneous enzyme immunoassay for theophylline these antibodies show insignificant cross-reactivity (less than 1%) to 1-methyl- and 1,3-dimethyluric acid, 3-methylxanthine, caffeine, and theobromine. In contrast, immunogens prepared from the C-8 functionalized drug afford antibodies which show more serious cross-reactivity to these compounds. Plausible rationale for attachment of the drug to carrier proteins through its N-3 position which furnished specific antibodies are given.

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Determination of theophylline in plasma by electron capture gas chromatography

Cited by 29 publications

References 11 publications

Synthesis and central nervous system actions of thyrotropin‐releasing hormone analogs containing a 1‐substituted 2‐oxoimadazolidine moiety*

Synthesis and central nervous system actions of thyrotropin‐releasing hormone analogs containing a 1‐substituted 2‐oxoimadazolidine moiety*

Anti-inflammatory effect of LA 2851 and reference drugs on some models of inflammation. Investigation of the mechanism of action

Specific Antibodies to Theophylline for Use in a Homogeneous: Enzyme Immunoassay

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