Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides

Abstract

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

“…However, the quality and characteristics of plant raw materials depend on different indicators (geographic, climatic, agro-technological, etc. ); therefore, it is essential to study medicinal plants from different growing areas [ 16 ]. This work aimed to study in vitro the properties and characteristics of extracts from Cotinus coggygria, growing in the Russian region of the Baltics.…”

Bioactive Compounds from Natural Products: Separation, Characterization, and Applications

“…In [11], chromatographic and NMR methods described the chemical composition of extracts from callus, cell suspension, and Rhaponticum carthamoides roots. Flavonoids, such as ecdysteroids and anthocyanins, can inhibit the proliferation of tumor cells and have other biological effects, which can be used in the future to produce biopharmaceuticals.…”