Determination of the parent of origin in nine cases of prenatally detected chromosome aberrations found after intracytoplasmic sperm injection

Abstract

Prenatal cytogenetic analysis of 71 fetuses conceived by intracytoplasmic sperm injection (ICSI) resulted in the detection of nine (12.7%) chromosome aberrations including two cases of 47,XXY, four cases involving a 45,X cell line and three autosomal trisomies. Molecular analysis of the parental origin of the deleted or supernumerary chromosome was performed by using polymorphic microsatellite markers. Six cases involving a sex chromosome abnormality were found to be of paternal origin while the two trisomic cases that could be analysed were of maternal origin. Two cases involved the same infertile couple who had two consecutive ICSI pregnancies terminated because of a chromosome abnormality. The replaced embryos in both cases originated from a single batch of ICSI fertilized oocytes of which part was used to initiate the first pregnancy and part was cryopreserved and used to initiate the second pregnancy.

The high incidence of meiotic errors increases with decreased sperm count in severe male factor infertilities

“…The much higher incidences of chromosomal abnormalities after ICSI reported by In't Veld et al (1995) and Van Opstal et al (1997) are consistent with our data. Previously, Martin (1996) suggested that there may be a risk of transmitting chromosomal abnormalities to offspring following ICSI.…”

Sperm Chromosomal Abnormalities In Patients With Unexplained Recurrent Abortions

“…Increased gonosomal aneuploidies were also reported in the sperm of oligoasthenoteratozoospermic patients [23]. Van Opstal and colleagues [33] prenatally evaluated 9 ICSI pregnancies and found out that 7 were paternally transmitted chromosome aberrations and 6 cases were sex chromosome abnormalities.…”

High Frequency of Sex Chromosomal Disomy in Spermatozoa of Lebanese Infertile Men

“…Oligozoospermic men are frequent candidates for ICSI, which is known to be a more invasive procedure than routine IVF (Devroey and Van Steirteghem, 2004). Some studies have reported a higher risk for de novo chromosomal anomalies of paternal origin (Van Opstal et al, 1997) in fetuses (Van Steirteghem et al, 2002) and children (Aboulghar et al, 2001) issued from the ICSI procedure. These aberrations are likely related to the high aneuploidy rates observed in the sperm of infertile men.…”