Determination of the dose-dependent toxic effects of mad honey on mouse liver using ATR-FTIR spectroscopy

Çakmak-Arslan, Gülgün; Haksoy, Hümeyra; Goc-Rasgele, Pinar; Kekeçoğlu, Meral

doi:10.1016/j.saa.2019.117719

Cited by 20 publications

(39 citation statements)

References 58 publications

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“…In the case of thick or strongly absorbing SD samples, attenuated total reflectance FTIR (ATR-FTIR) spectroscopy, which has been utilized widely as a powerful technique in recent SD studies and the analysis of biopharmaceuticals, is recommended to overcome the intense peaks that are produced by these samples [ 67 , 68 , 69 , 70 ]. This system consists of an FTIR spectroscope and an ATR accessory to create an evanescent wave that is then directed into the sample [ 71 ]. Contact between an ATR crystal surface and a sample will affect the quality of measurement [ 72 ].…”

Section: Spectroscopic Techniques Used In the Investigation Of Molmentioning

confidence: 99%

Molecular Interactions in Solid Dispersions of Poorly Water-Soluble Drugs

Tran

2020

Pharmaceutics

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Physicochemical characterization is a crucial step for the successful development of solid dispersions, including the determination of drug crystallinity and molecular interactions. Typically, the detection of molecular interactions will assist in the explanation of different drug performances (e.g., dissolution, solubility, stability) in solid dispersions. Various prominent reviews on solid dispersions have been reported recently. However, there is still no overview of recent techniques for evaluating the molecular interactions that occur within solid dispersions of poorly water-soluble drugs. In this review, we aim to overview common methods that have been used for solid dispersions to identify different bond formations and forces via the determination of interaction energy. In addition, a brief background on the important role of molecular interactions will also be described. The summary and discussion of methods used in the determination of molecular interactions will contribute to further developments in solid dispersions, especially for quick and potent drug delivery applications.

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Section: Spectroscopic Techniques Used In the Investigation Of Molmentioning

confidence: 99%

Molecular Interactions in Solid Dispersions of Poorly Water-Soluble Drugs

Tran

2020

Pharmaceutics

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“…They observed that it did not produce any significant changes in the liver tissue, except for a decrease in glycogen content. However, they also showed that 75 mg/kg mad honey and grayanotoxin-III caused significant changes to the composition, structure, and function of the liver tissue [4].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Properties and Phytochemical Screening of Mad Honey Bee Pollen

2022

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Introduction: Mad honey is widely used in folk medicine in the Black Sea region of Turkey for its medicinal properties, but its pollen is not collected by mad honey producers and its benefits are unknown. Methods: In this study, water and ethanol extracts of mad honey bee pollen from four different plateaus in the Black Sea region of Turkey were researched for their antioxidant and antimicrobial properties. Phytochemical analyses were carried out and the data were supported with UV-Vis spectrophotometry. Antimicrobial activity was researched against four Gram-positive and four Gram-negative bacteria, one fungus, and one yeast. The results were compared with standard antioxidants and antibiotics. Results: The ethanol extracts of the samples from Sinop (P5), Giresun (P7), and Trabzon (P8) were the most active samples as antioxidants, and almost all the extracts of R. ponticum bee pollen were effective on the tested microorganisms. However, P5–8 showed better values for B. cereus, E. feacalis, E. coli, and P. aeruginosa. Conclusion: It has been proven that mad honey bee pollen is a natural product with previously unknown medicinal properties and rich phytochemical content. Future research and clinical trials are important to scientifically support its benefits in complementary medicine.

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“…In FTIR spectrometers by utilizing the Attenuated Total Reflectance (ATR) unit, it is possible to determine such alterations more quickly and accurately by minimizing sample preparation or by examining samples directly, regardless of sample thickness. Detection of alterations in the band area ratios, wavenumbers and bandwidths of ATR-FTIR bands gives information about the amounts of biomolecules, their interactions with each other, the level of lipid peroxidation and secondary structures of proteins in a tissue (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Doksorubisinin Karaciğer Dokusunda Oluşturduğu Hasarın ve Propolisin Bu Hasar Üzerindeki Potansiyel Koruyucu Etkisinin Değerlendirilmesi

ORAK

ARSLAN

Kaya

2022

Konuralp Tıp Dergisi

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Objective: Doxorubicin (DOX), one of the chemotherapeutic drugs utilized in cancer treatment, has limited clinical use due to its serious toxic effects on non-target organs. The purpose of this study is to reveal the harmful effects of DOX in rat liver and the possible protective effect of propolis (PRPLS), a mixture of various herbal products collected by honeybees, on these damages by Attenuated Total Reflection-Fourier Transformation Infrared (ATR-FTIR) spectroscopy. Methods: Sprague dawley rats were separated into 4 groups; control, DOX (cumulative dose: 15 mg/kg), PRPLS (200 mg/kg) and DOX + PRPLS. The rats were given 200 mg/kg PRPLS by oral gavage daily for 20 consecutive days and 2.5 mg/kg DOX intraperitoneally on days 10, 12, 14, 16, 18 and 20 of the experiment. 24 hrs after the last administrations, liver samples were collected and examined by ATR-FTIR spectroscopy. Results: DOX caused a decrease in the amount of glycogen and nucleic acids, an increase in the amount of lipids and proteins and some important changes in the metabolism, structure and conformation of these molecules in the liver. DOX also induced lipid peroxidation, an increase in membrane fluidity, a decrease in membrane order and protein denaturation. PRPLS did not induce any toxic effect on the liver when it was given alone and PRPLS administered before DOX was not effective to eliminate these harmful effects of DOX. Conclusions: DOX caused significant structural and compositional changes in liver tissue and PRPLS was inadequate to prevent these changes at the dose and time used here.

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Determination of the dose-dependent toxic effects of mad honey on mouse liver using ATR-FTIR spectroscopy

Cited by 20 publications

References 58 publications

Molecular Interactions in Solid Dispersions of Poorly Water-Soluble Drugs

Molecular Interactions in Solid Dispersions of Poorly Water-Soluble Drugs

Bioactive Properties and Phytochemical Screening of Mad Honey Bee Pollen

Doksorubisinin Karaciğer Dokusunda Oluşturduğu Hasarın ve Propolisin Bu Hasar Üzerindeki Potansiyel Koruyucu Etkisinin Değerlendirilmesi

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