Determination of serum lipid peroxide levels in healthy and diseased subjects by use of a methylene blue derivative.

Tateishi, Tohru; Yoshimine, Noboru; Kuzuya, Fumio

doi:10.3164/jcbn.7.169

Cited by 4 publications

(7 citation statements)

References 9 publications

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“…(Hb-MB) and their correlation with the clinical parameters [2]. In that study the LPO level was higher in hypertensive patients than in healthy subjects and there was no age-related change in the level.…”

Section: Discussionmentioning

confidence: 52%

Measurement of Lipid Peroxide Levels in Erythrocyte Ghosts from Patients with Various Diseases.

Yoshimine

Tateishi

Ando

et al. 1992

J. Clin. Biochem. Nutr.

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By the method using a methylene blue derivative, we investigated the hydroperoxide (LPO) level of erythrocyte membranes (EG) in patients suffering from diabetes mellitus, hypertension, cerebral stroke, hypercholesterolemia, and ischemic heart disease. In addition, the correlation between the EG-LPO level and the clinical parameters was analyzed. The EG-LPO level registered 1.6 ±0.9 nmol/mg protein in the healthy controls, whereas higher EG-LPO levels were obtained in the diabetic and the hypertensive patients. However, the EG-LPO in the hypercholesterolemic patients showed lower value than that in the diabetic, from which we would suggest the inhibitory effect of high cholesterol on lipid peroxidation. There was no correlation between the EG-LPO level and the clinical parameters. The lipid peroxide in the erythrocyte membrane would be supposed to be one of the independent vascular risk factors, and especially the high values of the EG-LPO in the diabetic and hypertensive patients might be related to the high incidence of vascular accidents in these diseases.

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Section: Discussionmentioning

confidence: 52%

Measurement of Lipid Peroxide Levels in Erythrocyte Ghosts from Patients with Various Diseases.

Yoshimine

Tateishi

Ando

et al. 1992

J. Clin. Biochem. Nutr.

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“…In previous studies [2,3], we reported finding high values of serum LPO and EG-LPO in patients with hypertension or diabetes mellitus. Particularly in the hypertensives, we concluded that the high LPO levels in serum and the EG-LPO might damage tissues and organs.…”

Section: Discussionmentioning

confidence: 99%

“…Erythrocyte membranes readily undergo lipid oxidation and protein crosslinking by various peroxides [1]. Using a derivative of methylene blue (Hb-MB) and correlating the findings with clinical parameters such as age, blood pressure, routine blood chemistry, serum lipids, and serum lipid peroxide level, we previously reported lipid peroxide (LPO) levels of serum and erythrocyte ghosts (EG) in patients with vascular diseases [2]. Although no significant correlation between the EG-LPO level and any of the clinical parameters was observed, the level of the EG-LPO was higher in patients with hypertension or diabetes mellitus [3], suggesting that the metabolism of lipid peroxidation of erythrocyte membranes might play an important pathophysiological role in diabetes mellitus, hypertension, and other vascular diseases.…”

mentioning

confidence: 99%

Effect of Exercise Testing on Lipid Peroxide Levels of Plasma and Erythrocyte Ghosts.

Yoshimine

Goto

Hayashi

et al. 1993

J. Clin. Biochem. Nutr.

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SummaryThis study was designed to detect whether the plasma level of lipid peroxide and of erythrocyte ghost (EG) would be affected by treadmill exercise testing carried out by the modified Bruce method in patients with hypertension or hypercholesterolemia. A total of 27 patients were studied for changes in their ECG. On exercise testing, 10 patients responded positively and 17 negatively. Before testing, plasma levels of lipid peroxide were 3.9 ±0.9 nmol/ml (mean ± SE) in the positive group and 1.6 ± 0.4 nmol/ml in the negative group, a significant difference (p < 0.05). The lipid peroxide (LPO) level of the EG was 3.7±0.8 nmol/mg protein in the positive group and 3.2 ±0.7 nmol/mg protein in the negative group. Exercise testing tended to induce a rise in the lipid peroxide values in the EG of the positive group (11.3±5.0 nmol/mg protein vs. 3.7 ±0.8 nmol/mg protein, p<0.1). The fold-increase in the level of erythrocyte membrane lipid peroxide was significantly higher in the positive responders (2.3±0.7 vs. 0.8±0.1, p<0.05). There was no significant change in the plasma level of lipid peroxide in either group. Other clinical parameters including blood pressure, heart rate, obesity, smoking, medication, exercise intensity, and plasma lipid levels showed no differences between the positive and the negative responders. We conclude that the erythrocyte membranes of the positive responders to treadmill exercising are easily peroxidized by various stimuli, which may contribute in part to the high plasma lipid peroxide level before testing. A stimulus such as treadmill testing may accelerate the lipid peroxidation of the erythrocyte membranes significantly and independently.

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“…Malondialdehyde (MDA) formed from 1,1,3,3-tetramethoxypropane was used as a standard. LPO in 100 gl of sample was measured with a Determiner LPO kit (Kyowa Medex, Tokyo, Japan), as previously described [16][17][18]. Cumen hydroperoxide was used as a standard.…”

Section: Assays For Products Of Lipid Peroxidationmentioning

confidence: 99%

“…Cumen hydroperoxide was used as a standard. The fluorescence intensity of each lipoprotein was measured on a spectrofluorometer (Hitachi F-3010), with excitation at 400nm and emission at 470nm, as protein adducts of reactive aldehydes [17].…”

Section: Assays For Products Of Lipid Peroxidationmentioning

confidence: 99%

Chelation of copper reduces inhibition by oxidized lipoproteins of endothelium-dependent relaxation in porcine coronary arteries

et al. 1994

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We examined the effect of dialyzing oxidized low-density lipoprotein (oLDL) against Krebs-Ringer solution, in the absence (yielding d-oLDL) or presence (yielding EDTA-oLDL) of ethylenediamine tetraacetic acid (EDTA), to investigate the mechanism that underlies the inhibition of endothelium-dependent relaxation (EDR) by o-LDL. Oxidation of LDL by exposure to Cu2+ resulted in the formation of a thiobarbituric acid-reacting substance (TBARS) and lipid hydroperoxide (LPO). At a concentration of 5 mg/dl, d-oLDL markedly attenuated EDR in the porcine coronary artery. Analysis of d-oLDL by gel filtration revealed that TBARS was ditributed in both the lipoprotein and the aqueous phases, whereas LPO was present only in the lipoprotein particles. Lysophosphatidylcholine (LPC), which has been suggested to be responsible for the impairment of EDR by oLDL, was present not only in the lipoprotein but also in the aqueous phase. However, EDR inhibitory activity was observed only in the oLDL particles, not in the aqueous phase. Almost all Cu2+ associated with the oLDL particles was removed by dialysis of oLDL against Krebs-Ringer solution containing EDTA. EDTA-oLDL or native LDL, at concentrations as high as 75 mg/dl, exerted only a moderate inhibitory action on EDR, Both TBARS and LPO in EDTA-oLDL were distributed only in the lipoprotein particles, not in the aqueous phase. These results demonstrate that the impairment of EDR by oLDL is related both to LPO and to transition metal ions such as Cu2+ associated with the lipoprotein particles, not to the amount of the TBARS or negative charge, and that factors other than LPC may affect EDR.

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Determination of serum lipid peroxide levels in healthy and diseased subjects by use of a methylene blue derivative.

Cited by 4 publications

References 9 publications

Measurement of Lipid Peroxide Levels in Erythrocyte Ghosts from Patients with Various Diseases.

Measurement of Lipid Peroxide Levels in Erythrocyte Ghosts from Patients with Various Diseases.

Effect of Exercise Testing on Lipid Peroxide Levels of Plasma and Erythrocyte Ghosts.

Chelation of copper reduces inhibition by oxidized lipoproteins of endothelium-dependent relaxation in porcine coronary arteries

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