Measurement of Lipid Peroxide Levels in Erythrocyte Ghosts from Patients with Various Diseases.

Abstract

By the method using a methylene blue derivative, we investigated the hydroperoxide (LPO) level of erythrocyte membranes (EG) in patients suffering from diabetes mellitus, hypertension, cerebral stroke, hypercholesterolemia, and ischemic heart disease. In addition, the correlation between the EG-LPO level and the clinical parameters was analyzed. The EG-LPO level registered 1.6 ±0.9 nmol/mg protein in the healthy controls, whereas higher EG-LPO levels were obtained in the diabetic and the hypertensive patients. However, the EG-LPO in the hypercholesterolemic patients showed lower value than that in the diabetic, from which we would suggest the inhibitory effect of high cholesterol on lipid peroxidation. There was no correlation between the EG-LPO level and the clinical parameters. The lipid peroxide in the erythrocyte membrane would be supposed to be one of the independent vascular risk factors, and especially the high values of the EG-LPO in the diabetic and hypertensive patients might be related to the high incidence of vascular accidents in these diseases.

Effect of Exercise Testing on Lipid Peroxide Levels of Plasma and Erythrocyte Ghosts.

“…In previous studies [2,3], we reported finding high values of serum LPO and EG-LPO in patients with hypertension or diabetes mellitus. Particularly in the hypertensives, we concluded that the high LPO levels in serum and the EG-LPO might damage tissues and organs.…”

“…EG were prepared by the method of Burton et al [6]. The details of this procedure were reported in our previous study [3]. In brief, the EG-and plasma LPO levels were measured with a Determiner LPO kit (Kyowa Medex, Tokyo), which utilizes the Hb-MB method [7].…”

“…Using a derivative of methylene blue (Hb-MB) and correlating the findings with clinical parameters such as age, blood pressure, routine blood chemistry, serum lipids, and serum lipid peroxide level, we previously reported lipid peroxide (LPO) levels of serum and erythrocyte ghosts (EG) in patients with vascular diseases [2]. Although no significant correlation between the EG-LPO level and any of the clinical parameters was observed, the level of the EG-LPO was higher in patients with hypertension or diabetes mellitus [3], suggesting that the metabolism of lipid peroxidation of erythrocyte membranes might play an important pathophysiological role in diabetes mellitus, hypertension, and other vascular diseases. Although the level of the EG-LPO in stable patients with old myocardial infarction did not show any significant difference from the control, we suppose that exercise loading could affect the circulatory balance of the coronary artery and induce lipid peroxidation of the erythrocyte membrane.…”

Alterations of lipid peroxide levels in the plasma and erythrocyte ghosts under treatment with simvastatin

“…[5], and EG-LPO was measured using a Determiner LPO kit. Details of this method were reported previously [2].…”

“…Erythrocyte membranes or ghosts (EG) readily undergo lipid oxidation and protein crosslinking by various peroxides [ 1 ]. We have previously evaluated the level of EG lipid peroxide (LPO) in various diseases, and reported that it was increased in patients with hypertension and diabetes mellitus in a manner independent of other clinical parameters [2]. Also, we have measured the effect of exercise (treadmill test) on the level of the EG-LPO in patients with hyperlipidemia or hypertension, and concluded that exertion increased the level of EG-LPO [3].…”