Determination of reliability and practicality of saliva as a genetic source in forensic investigation by analyzing DNA yield and success rates: A systematic review

Sundararajan, G.; Muruganandhan, Jayanandan; Priya, V. Vishnu; Srinivasan, Manali Ramakrishnan

doi:10.1016/j.ajoms.2018.12.004

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…The sources of DNA include soft tissues such as human skin [12], dental pulp odontoblastic processes [13], and cellular cementum [14]. Body fluids that are rich sources of DNA include human saliva, which contains desquamated epithelial cells and can be obtained in a non-invasive manner [15]. Other external sources of human DNA include saliva-stained stamps, cigarette butts [16], toothbrushes, and oral prostheses [17].…”

Section: Introductionmentioning

confidence: 99%

Toothbrushes as a Source of DNA for Gender and Human Identification—A Systematic Review

Sundararajan

Priya

Dubey

et al. 2021

IJERPH

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Background: Few studies have reported the use of toothbrushes as a reliable source of DNA for human or gender identification. The present systematic review with the available information was conducted to answer the focus question “Is a toothbrush a reliable source of DNA for human or gender identification?”. Methods: The keyword combination “Toothbrush” and “DNA” was used to search databases including MEDLINE, Scopus, and Web of Science along with a manual search of reference lists of relevant articles. Duplicates and irrelevant articles were excluded, and the remaining articles were fully read for the final selection of articles. The risk of bias of the included studies was evaluated using the Appraisal tool for Cross-Sectional Studies (AXIS tool). Results: Of the 130 articles obtained, 122 duplicates or irrelevant articles were eliminated. Following the full-text reading of eight articles, five articles were selected based on eligibility criteria. The five studies reported that a toothbrush is a good source of DNA irrespective of the time interval. In a few studies some samples were not sufficient for complete DNA profiling due to factors such as the method of DNA extraction. Conclusion: Although a toothbrush is an excellent source of DNA for human and gender identification, future studies with a larger sample size, appropriate control group, and standardized technique of DNA extraction need to be conducted. Additionally, factors influencing the quantity and quality of DNA in toothbrushes need to be determined with standardized techniques.

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Section: Introductionmentioning

confidence: 99%

Toothbrushes as a Source of DNA for Gender and Human Identification—A Systematic Review

Sundararajan

Priya

Dubey

et al. 2021

IJERPH

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“…Forensic analysis has successfully improved public security by systematically collecting trace evidence. 1,2 One of the main areas of focus in forensic investigations are the bodily fluids obtained at a crime scene, 3,4 including saliva, 5 urine, 6 blood, 7 and sweat. 8 Among fluid types, blood is the most commonly studied piece of biological evidence.…”

Section: Introductionmentioning

confidence: 99%

Latent bloodstain detection using a selective turn-on NIR fluorescence dye responsive to serum albumin

Qu,

Meador,

Cheah

et al. 2023

RSC Adv.

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“…A similar principle applies to Van Gieson staining technique [5]. Our extensive slide collection has enabled us to publish numerous articles in the past 3 years [6][7][8][9][10][11][12][13]. Based on these inspirations, we have planned to compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson's Trichrome to employ them for better histological grading of the disease.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study

Umashankar

Sherlin

Jayaraj

et al. 2021

BDS

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Background: Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features could be identified in routine Hematoxylin and Eosin (H and E) staining in shades of pink. However, the use of special stains provides a contrast to various connective tissue components thereby aiding in better visualization of these connective tissue changes in advanced OSMF cases. Objective: To compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson’s Trichrome. Material and Methods: 10 Formalin-fixed paraffin-embedded tissue sections of advanced OSMFwere stained using 3 different stains namely Hematoxylin and Eosin (H&E), Van-Gieson, and Masson trichrome. The results obtained were tabulated and statistically analyzed using Kruskal Wallis ANOVA. Results: The hyalinization and fibrosis involving the skeletal muscle were better visualized in Masson’s Trichrome but were not statistically significant. The muscle degeneration in deeper areas was better visualized in Masson’s trichrome when compared to the H&E and Van Gieson. Conclusion: Hematoxylin and eosin stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Masson’s trichrome stain can be used as a single adjunct to hematoxylin and eosin stain as changes in the superficial and deeper connective tissue could be ascertained. Keywords Fibrosis; Masson’s trichrome stain; Muscle degeneration; Oral submucous fibrosis grading.

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Determination of reliability and practicality of saliva as a genetic source in forensic investigation by analyzing DNA yield and success rates: A systematic review

Cited by 4 publications

References 22 publications

Toothbrushes as a Source of DNA for Gender and Human Identification—A Systematic Review

Toothbrushes as a Source of DNA for Gender and Human Identification—A Systematic Review

Latent bloodstain detection using a selective turn-on NIR fluorescence dye responsive to serum albumin

Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains – a retrospective cross-sectional study

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