Determination of quinolone residues in raw cow milk. Application of polar stir-bars and ultra-high performance liquid chromatography–tandem mass spectrometry

Rodríguez-Gómez, R.; García-Córcoles, María Teresa; Çipa, Morsina; Barrón, D.; Zafra-Gómez, A.; Zafra-Gómez, Alberto

doi:10.1080/19440049.2018.1430382

Cited by 23 publications

(5 citation statements)

References 30 publications

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“…Although it is a bit less sensitive than other previously reported approaches, it has significantly reduced time, sample consumption, and labor cost (Table S1). − The whole chip–MS analysis procedure developed here took approximately 30 min per sample, whereas it takes more than 90 min to analyze one sample with the traditional LC-MS method. Moreover, less than 100 μL of organic reagents was needed for the developed method.…”

Section: Resultsmentioning

confidence: 99%

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

et al. 2019

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In this work, a highly integrated microfluidic chip with multifunction coupled to mass spectrometry (MS) was developed for online analysis of seven different regulated quinolones (QNs) in milk samples. Procedures of sample extraction, immunoaffinity enrichment, magnetic separation, and online elution were performed simultaneously on the specifically designed device. Based on the specificity of antibodies, direct (electrospray ionization) ESI-MS at full scan mode without liquid chromatography (LC) separation and further tandem mass spectrometry (MS/MS) analysis was developed for the identification of target QNs. One single isotope internal standard (IS) method was presented for quantitative analysis of seven QNs. Upon targeted online extraction and enrichment by antibody conjugated magnetic beads, seven QNs were quantitatively determined by the IS method with the linear range of 0.2/0.5–10 ng/mL (R 2 > 0.991). The limits of detection (LODs) for the seven QNs were in the range of 0.047–0.490 ng/mL. This system permits automated on-chip immunoaffinity enrichment and accurate MS detection without additional off-line cleanup procedures.

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Section: Resultsmentioning

confidence: 99%

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

et al. 2019

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“…SBSE has a large preconcentration capacity, requires relatively low sample volumes and can be run overnight without any special requirements. Due to its large volume and surface area, the extraction capacity is higher than that of SPME [54,55]. Because SBSE is not convenient for in vivo or on-site sampling, the application of SBSE based on a MIP coating for antibiotics is less frequent [56].…”

Section: Spe Modes Based On Mipsmentioning

confidence: 99%

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

et al. 2023

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The abuse and residues of antibiotics have a great impact on the environment and organisms, and their determination has become very important. Due to their low contents, varieties and complex matrices, effective recognition, separation and enrichment are usually required prior to determination. Molecularly imprinted polymers (MIPs), a kind of highly selective polymer prepared via molecular imprinting technology (MIT), are used widely in the analytical detection of antibiotics, as adsorbents of solid-phase extraction (SPE) and as recognition elements of sensors. Herein, recent advances in MIPs for antibiotic residue analysis are reviewed. Firstly, several new preparation techniques of MIPs for detecting antibiotics are briefly introduced, including surface imprinting, nanoimprinting, living/controlled radical polymerization, and multi-template imprinting, multi-functional monomer imprinting and dummy template imprinting. Secondly, several SPE modes based on MIPs are summarized, namely packed SPE, magnetic SPE, dispersive SPE, matrix solid-phase dispersive extraction, solid-phase microextraction, stir-bar sorptive extraction and pipette-tip SPE. Thirdly, the basic principles of MIP-based sensors and three sensing modes, including electrochemical sensing, optical sensing and mass sensing, are also outlined. Fourthly, the research progress on molecularly imprinted SPEs (MISPEs) and MIP-based electrochemical/optical/mass sensors for the detection of various antibiotic residues in environmental and food samples since 2018 are comprehensively reviewed, including sulfonamides, quinolones, β-lactams and so on. Finally, the preparation and application prospects of MIPs for detecting antibiotics are outlined.

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“…According to previous papers, a variety of analytical methods for the racemic determination of flumequine in animal productions (including milk, honey, egg, fish, shrimp, chicken, pork, and beef) have been published using capillary electrophoresis (CE), 6,7 CE tandem mass spectrometry (CE-MS/MS), 8,9 liquid chromatographyultraviolet detection (LC-UV), [10][11][12][13][14][15] and liquid chromatography tandem mass spectrometry (LC-MS/MS). [16][17][18][19][20][21] LC-UV and LC-MS have been widely utilized for quantification analysis of flumequine. For example, Zhao et al 10 developed a method for 10 quinolones in swine, chicken, and shrimp muscle by LC-UV.…”

Section: Abbreviationsmentioning

confidence: 99%

Enantioseparation and determination of flumequine enantiomers in multiple food matrices with chiral liquid chromatography coupled with tandem mass spectrometry

Liu

Xue

et al. 2019

Chirality

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The present work firstly described the enantioseparation and determination of flumequine enantiomers in milk, yogurt, chicken, beef, egg, and honey samples by chiral liquid chromatography‐tandem mass spectrometry. The enantioseparation was performed under reversed‐phase conditions on a Chiralpak IC column at 20°C. The effects of chiral stationary phase, mobile phase components, and column temperature on the separation of flumequine enantiomers have been studied in detail. Target compounds were extracted from six different matrices with individual extraction procedure followed by cleanup using Cleanert C18 solid phase extraction cartridge. Good linearity (R2>0.9913) was obtained over the concentration range of 0.125 to 12.5 ng g‐1 for each enantiomer in matrix‐matched standard calibration curves. The limits of detection and limits of quantification of two flumequine enantiomers were 0.015‐0.024 and 0.045‐0.063 ng g‐1, respectively. The average recoveries of the targeted compounds varied from 82.3 to 110.5%, with relative standard deviation less than 11.7%. The method was successfully applied to the determination of flumequine enantiomers in multiple food matrices, providing a reliable method for evaluating the potential risk in animal productions.

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Determination of quinolone residues in raw cow milk. Application of polar stir-bars and ultra-high performance liquid chromatography–tandem mass spectrometry

Cited by 23 publications

References 30 publications

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

Highly Integrated Microfluidic Chip Coupled to Mass Spectrometry for Online Analysis of Residual Quinolones in Milk

Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis

Enantioseparation and determination of flumequine enantiomers in multiple food matrices with chiral liquid chromatography coupled with tandem mass spectrometry

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