Determination of prednisolone and the most important associated compounds in ocular and cutaneous pharmaceutical preparations by micellar electrokinetic capillary chromatography

Gallego, Jorge; Arroyo, J. Pérez

doi:10.1016/s1570-0232(02)00748-1

Cited by 31 publications

(21 citation statements)

References 31 publications

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“…The analytes were separated in a 5 mM phosphate-5 mM borate buffer, pH 8.2, containing 50 mM SDS and 3% v/v CH 3 CN, with detection at 205 nm. The level of sulfacetamide sulfate found, 30.5 mg/mL, agreed well with the declared level of 32.0 mg/mL, and the 60.5 mg/mL zinc bacitracin found agreed well with the 63.8 mg/mL declared [24].…”

Section: Ointmentssupporting

confidence: 72%

“…Under these conditions, the bacitracin complex elutes in one peak, simplifying quantitation. The method is linear from 5.0 to 73.4 mg/mL, with an LOQ of 5.0 mg/mL, and an LOD of 1.5 mg/mL [24]. The determination of the amount of bacitracin present in a nasal ointment was demonstrated [24].…”

Section: Polypeptidesmentioning

confidence: 99%

“…MEKC was also used for the determination of zinc bacitracin in a mixture of compounds that can be found in ocular or nasal preparations [24]. Prednisolone, prednisolone acetate, zinc bacitracin, sulfacetamide sulfate, phenylephrine and naphazoline nitrate were separated in a 5 mM phosphate-5 mM borate buffer, pH 8.2, containing 50 mM SDS and 3% v/v CH 3 CN, with detection at 205 nm.…”

Section: Polypeptidesmentioning

confidence: 99%

“…MEKC was used for the determination of sulfacetamide sulfate in a mixture of compounds that can be found in ocular preparations [24]. A commercial eye drop sample containing prednisolone acetate, sulfacetamide sulfate and phenylephrine was diluted with 30% MeOH.…”

Section: Eye Dropsmentioning

confidence: 99%

“…MEKC was used for the determination of sulfacetamide sulfate and zinc bacitracin in a mixture of compounds that can be found in ocular and nasal ointments [24].…”

Section: Ointmentsmentioning

confidence: 99%

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Analysis of antibiotics by capillary electrophoresis

Flurer

2003

Electrophoresis

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Recent developments in the characterization of antibiotics are reviewed. Many capillary electrophoretic techniques have been utilized in their analyses, addressing various aspects of quantifying, profiling, and monitoring. Laser-induced fluorescence detection systems demonstrated their usefulness in clinical settings and in the monitoring of residue levels in food matrices. Different sample introduction methods have been explored, enhancing detection sensitivity, or reducing or eliminating sample manipulation prior to injection.

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Section: Ointmentssupporting

confidence: 72%

Section: Polypeptidesmentioning

confidence: 99%

Section: Polypeptidesmentioning

confidence: 99%

Section: Eye Dropsmentioning

confidence: 99%

“…MEKC was used for the determination of sulfacetamide sulfate and zinc bacitracin in a mixture of compounds that can be found in ocular and nasal ointments [24].…”

Section: Ointmentsmentioning

confidence: 99%

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Analysis of antibiotics by capillary electrophoresis

Flurer

2003

Electrophoresis

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Online naphazoline quality control by micellar‐enhanced spectrofluorimetry

et al. 2011

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The aim of this study was to develop a method for online spectrofluorimetric quality control of naphazoline (NPZ) in pharmaceuticals and raw drugs. A combination of a flow-injection analysis (FIA) system with micellar-enhanced fluorescence detection is presented as a powerful alternative for the rapid and sensitive analysis of naphazoline. Since NPZ shows low native fluorescence, the use of an anionic surfactant, such as sodium dodecyl sulphate (SDS), provides a considerable enhancement of fluorescence intensity and the nature of the technique allows a possible and easy adaptation to a FIA system. Using λ(exc) = 280 nm and λ(em) = 326 nm, a good linear relationship (LOL) was obtained in the range 0.003-10 µg mL(-1) with a detection limit (LOD) of 3 × 10(-4) µg mL(-1) (s/n = 3). Parameters related to the nature of the analytical signal and to the FIA manifold were optimized. Satisfactory recoveries were obtained in the analysis of commercial pharmaceutical formulations. The proposed method is simple, accurate and allows for high-speed sampling and considerably shorter analysis times. In addition, it requires inexpensive equipment and reagents and has easy operational conditions and no side effects, thus avoiding environmental pollution through toxic waste.

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Recent advances in micellar electrokinetic chromatography

2005

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This review contains nearly 200 reference citations, and covers advances in electrokinetic capillary chromatography based on micelles, including stabilized micelle complexes, polymeric and mixed micelles from 2003-2004. Detection strategies, analyte determinations, and applications in micellar electrokinetic capillary chromatography (MEKC) are discussed. Information regarding methods of analyte concentration, analyte specific analyses, and nonstandard micelles has been summarized in tabular form to provide a means of rapid access to information pertinent to the reader.

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Determination of prednisolone and the most important associated compounds in ocular and cutaneous pharmaceutical preparations by micellar electrokinetic capillary chromatography

Cited by 31 publications

References 31 publications

Analysis of antibiotics by capillary electrophoresis

Analysis of antibiotics by capillary electrophoresis

Online naphazoline quality control by micellar‐enhanced spectrofluorimetry

Recent advances in micellar electrokinetic chromatography

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