Analysis of antibiotics by capillary electrophoresis

Flurer, Cheryl L.

doi:10.1002/elps.200305639

Cited by 42 publications

(25 citation statements)

References 44 publications

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“…Several reviews have been published on the application of CE methods in food analysis in the last three years, which is a good demonstration of the possibilities of this technique in this particular field. Table 1 summarizes the different reviews published on this topic [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], including the aforementioned review by Frazier and Papadopoulou [1].…”

Section: Among the Different Analytical Techniques That Can Be Employmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

García‐Cañas

Cifuentes

2007

Electrophoresis

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Section: Among the Different Analytical Techniques That Can Be Employmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

García‐Cañas

Cifuentes

2007

Electrophoresis

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“…Additionally, the reviews published by Flurer describing recent advances in the analysis of antibiotics by CE, among which the FQs, presented an overview about the determination of this kind of pharmaceutical in different matrixes. [11][12][13] Due to the use of FQs for human infections and prophylactic treatment of some animal species used in food industry, the development and validation of methodology capable of improving the quality control of pharmaceutical formulations used for this purpose is very important.…”

Section: Introductionmentioning

confidence: 99%

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Faria¹,

Souza²,

Oliveira³

2008

J. Braz. Chem. Soc.

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Um método alternativo por eletroforese capilar de zona (CZE) para determinação de ciprofloxacina (CPFLX), gatifloxacina (GTFLX), moxifloxacina (MFLX) e ofloxacina (OFLX) foi validado. O sistema de eletrólito utilizado consistiu da mistura de 25 mmol L -1 de TRIS/ HCl e 15 mmol L -1 de tetraborato de sódio em meio aquoso resultando em pH 8,87. A análise foi realizada sob detecção direta por UV em 282 nm com tempo de análise de 3 min. Os parâmetros analíticos de validação avaliados foram: linearidade (r > 0,998), seletividade (comparação entre a inclinação da curva de calibração de padronização externa e curva de calibração de adição de padrão), repetitividade em área para amostras (RSD %: < 3,94% para CPFLX, < 3,87% para GTFLX, 1,30% para MFLX e < 1,88% para OFLX), precisão intermediária em área para amostras (RSD%: < 3,59% para CPFLX, < 3,09% para GTFLX, 2,67% para MFLX e < 2,25% para OFLX), exatidão (média da faixa de recuperação: 101,2% para CPFLX, 101,0% para GTFLX, 101,3% para MFLX e 99,9% para OFLX), limite de detecção (mg L -1 : 2,72 para CPFLX, 1,92 para GTFLX, 0,795 para MFLX e 1,05 para OFLX), limite de quantificação (mg L -1 : 9,06 para CPFLX, 6,40 para GTFLX, 2,65 para MFLX e 3,50 para OFLX) e robustez. Devido à simplicidade, seletividade, precisão, exatidão e rapidez, o método pode ser uma alternativa interessante para auxiliar o controle da qualidade dessas drogas na indústria farmacêutica.An alternative capillary zone electrophoresis (CZE) method for the determination of ciprofloxacin (CPFLX), gatifloxacin (GTFLX), moxifloxacin (MFLX) and ofloxacin (OFLX) through a simple aqueous electrolyte system consisting of 25 mmol L -1 of TRIS/ hydrochloride and 15 mmol L -1 of sodium tetraborate buffer mixture (pH 8.87) using direct UV detection at 282 nm within 3 min was validated. The analytical parameters of validation evaluated were: linearity (r > 0.998), selectivity (comparison between slope of the calibration curve of external standard and calibration curve of standard addition), repeatability in area for sample (RSD%: < 3.94% for CPFLX, < 3.87% for GTFLX, 1.30% for MFLX and < 1.88% for OFLX), intermediate precision in area for sample (RSD%: < 3.59% for CPFLX, < 3.09% for GTFLX, 2.67% for MFLX and < 2.25% for OFLX), accuracy (mean of recovery range: 101.2% for CPFLX, 101.0% for GTFLX, 101.3% for MFLX and 99.9% for OFLX), limit of detection (mg L -1 : 2.72 for CPFLX, 1.92 for GTFLX, 0.795 for MFLX and 1.05 for OFLX), limit of quantification (mg L -1 : 9.06 for CPFLX, 6.40 for GTFLX, 2.65 for MFLX and 3.50 for OFLX) and robustness. Due to its simplicity, selectivity, precision, accuracy and rapidity, the methodology can be an interesting alternative for quality assurance in the pharmaceutical industry of these drugs.

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“…Literature published before this date on this subject has already been reviewed by Flurer [2][3][4][5]. The experimental conditions employed to achieve the analysis of antibiotics by CE are first presented in this article.…”

Section: Introductionmentioning

confidence: 99%

“…The analysis of antibiotics by CE is mainly included in two different working modes: (i) CZE where a separation buffer without or with additives is used for the separation of ionic or ionogenic antibiotics based on their different electrophoretic mobilities, and (ii) MEKC where a micellar system (surfactant at a concentration higher than its CMC) is added to the separation buffer to perform the separation of neutral and/or ionic or ionogenic antibiotics based on the generation of a pseudostationary phase in which analyte partition takes place. Although much less used, CEC and nonaqueous CE (NACE) have also been used for the analysis of antibiotics [2]. …”

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confidence: 99%

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Recent advances in the analysis of antibiotics by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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Recent advances in the analysis of antibiotics by capillary electrophoresisIn this review, the main aspects related to the separation of different groups of antibiotics by CE as well as the different applications reported in the literature from the beginning 2003 till May 2005 will be provided to the readers. Firstly, the experimental conditions employed to achieve the analysis of antibiotics by CE are given. Then, the main applications performed in the pharmaceutical, clinical, food, and environmental fields have been reviewed making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Although HPLC is mainly used for the analysis of antibiotics by separation techniques, CE is being increasingly employed due to its favorable characteristics (high efficiency, large flexibility, and low consumption of samples and reagents). In addition, CE is being used in routine analysis because it allows obtaining appropriate analytical characteristics and good quantitative results. The analysis of antibiotics by CE is mainly included in two different working modes: (i) CZE where a separation buffer without or with additives is used for the separation of ionic or ionogenic antibiotics based on their different electrophoretic mobilities, and (ii) MEKC where a micellar system (surfactant at a concentration higher than its CMC) is added to the separation buffer to perform the separation of neutral and/or ionic or ionogenic antibiotics based on the generation of a pseudostationary phase in which analyte partition takes place. Although much less used, CEC and nonaqueous CE (NACE) have also been used for the analysis of antibiotics [2]. KeywordsIn order to provide to the readers an updated view of the separation conditions as well as the different applications reported in the analysis of antibiotics using CE as separation technique, this review covers the literature dealing on the analysis of antibiotics by CE from the beginning of 2003 till May 2005. Literature published before this date on this subject has already been reviewed by Flurer [2][3][4][5]. The experimental conditions employed to achieve the analysis of antibiotics by CE are first presented in this article. Section 3 of this work describes the main applications performed in the pharmaceutical, clinical, food, and environmental fields making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Analysis of antibiotics by CE

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Analysis of antibiotics by capillary electrophoresis

Cited by 42 publications

References 44 publications

Recent advances in the application of capillary electromigration methods for food analysis

Recent advances in the application of capillary electromigration methods for food analysis

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Recent advances in the analysis of antibiotics by capillary electrophoresis

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