Determination of pre and post treatment oxidative status and oxidative DNA damage in diarrheic calves

Abstract

Increase in production of free radicals causes damage in lipids of cell membrane, weakening in functions of cell proteins and DNA damage. The aim of the present study was to determine the oxidative status, level of oxidative DNA damage and treatment effectiveness on these parameters on diarrheic calves. The study was conducted using 10 clinically holstein calves from 2-3 months of age. Antidiarrheic treatment containing Amonium sulphate (30mg/kg/day, Gabbrocol, Ceva Inc.), a mixture of Bismuth subcarbonate, kaolin, pectin (10g/day, Bismol, Bioteknik Inc.), vitamin and mineral mixture (105g/day, Sky High Energy, Egevet Co.Ltd) as drugs orally administered to all calves for 3 days. Body temperature, feces and blood samples were taken before and after treatment. Dry matter measured (%) in feces, DNA damage level and some hematologic parameters, total oxidant status (TOS), total antioxidant status (TAS) and some biochemical parameters were measured from blood samples. HB, ALT, TP levels did not differ before and after treatment. However, body temperature (p<0.01), WBC (p<0.01), HCT (p<0.05), GGT (p<0.01), TOS (p<0.05), DNA damage (p<0.05) were decreased, dry matter in feces (p<0.01), RBC (p<0.05), AST (p<0.01), Albumin (p<0.01) and TAS (p<0.05) were increased after treatment. The results of the study indicate that oxidative DNA damage increases with degenerative diseases such as diarrhea and decreases to the normal range after effective treatment. It can be suggested from these results that oxidative DNA damage might be a good indicator to reveal the degeneration level of diarrhea in animals and a good parameter to evaluate the effectiveness of the treatment in terms of cellular form.

​A Study on Alteration in Serum Biochemical Parameters in Colibacillosis Affected Diarrhoeic Cattle Calves

“…There was a significant increase in the total oxidant status andoxidative stress index in the calves affected with colibacillosis, however, there was significant decrease in total oxidant status. Our findings are in agreement with the study conducted by Kabu et al (2015). Similarly, many researchers also reported an increase in the values of TOS in many other diseases.…”

“…Aslan et al (2011) demonstrated that TOS levels were significantly higher in patients with ulcerative colitis while TAC was significantly lower. This rise of total oxidant status and decreased TAC appeared to be possibly associated with stress caused by the disease (Kabu et al, 2015).…”

Evaluation of Oxidative Stress Index and Some Biochemical Parameters in Neonatal Calves with Diarrhea

“…As a result, the oxidative stress index increased. In many studies, it has been reported that oxidative stress occurs in diarrhea and septicemia that develops as a result [39][40][41]. When cells are exposed to oxidative stress, they increase the activity and expression of antioxidant enzymes to protect them from damage caused by free radicals [42].…”

Effects of eprinomectin administration on apoptosis, acute phase response and antioxidant status in cattle

“…Free radicals are produced in the body as by products of normal metabolism and as a result of exposure to radiation and some environmental pollutants. Free radicals are normally neutralized by efficient systems in the body that include the antioxidant defense system (Kabu et al, 2015;Sardesai, 1995). Low levels of antioxidants in blood reflect oxidative damage caused by free radicals (Joreno, 1990;Kanbur et al, 2008;Noori et al, 2018).…”