Determination of polyamines in organic extracts from roots ofCanavalia ensiformisby capillary electrophoresis

Santos, Soraya da Silva; Moraes, Maria de Lourdes Leite de; Rezende, Maria Olímpia de Oliveira

doi:10.1080/03601231003704481

Cited by 6 publications

(11 citation statements)

References 23 publications

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“…Observou-se ainda neste trabalho que ocorre um aumento na porcentagem de inibição de germinação de sementes das plantas daninhas utilizadas no trabalho ao se utilizar a mistura das substâncias citadas quando comparado aos bioensaios utilizando soluções de apenas um dos Podemos inferir com isso que é o efeito sinérgico das quatro substâncias presentes nas folhas de feijão-de-porco, ácido clorogênico e ácido p-anísico o responsável pelo controle de C. canadensis e C. bonariensis. Outros tecidos vegetais de C. ensiformis, como sementes e raízes tem sido investigados por alguns autores, revelando também substâncias alelopáticas contra espécies de plantas daninhas (Mendes e Rezende, 2014;Santos et al, 2007;Santos et al, 2010).…”

Section: Figura 2 Porcentagem De Controle De C Canadensis (Esquerdaunclassified

CONTROLE EM PÓS-EMERGÊNCIA DE Conyza canadensis e Conyza bonariensis COM EXTRATOS DE Canavalia ensiformis

2017

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Avaliou-se o efeito alelopático dos extratos aquoso e etanólico de folhas de Canavalia ensiformis no controle em pós-emergência das espécies Conyza canadensis e Conyza bonariensis. Para isso, foram preparados extratos aquoso e etanólico nas concentrações: 10, 25, 50, 100 e 150 g.L-1. Para a avaliação do efeito alelopático, os extratos foram aplicados no estádio de dois e quatro pares de folhas e a plântulas avaliadas aos 10 dias após as pulverizações com auxílio de uma escala de notas da Sociedade Brasileira De Ciência Das Plantas Daninhas. Ao final do experimento foi quantificado a massa fresca e seca em gramas das repetições referentes ao respectivo tratamento. Para ambas as espécies de daninha o extrato aquoso se destacou. A concentração de 150 g.L-1 inibiu 94% o desenvolvimento de C. bonariensis e as concentrações de 100 g.L-1 e 150 g.L-1 inibiram 96% e 100%, respectivamente as plântulas de C. canadensis, enquanto que o extrato etanólico controlou apenas 50% o desenvolvimento de C. bonariensis na mesma concentração e para C. canadensis alcançou apenas 4% e 12% nas respectivas concentrações. As matérias fresca e seca decresceram à medida que se aumentaram as doses do extrato aquoso de folhas de C. ensiformis, para ambas as espécies.

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Section: Figura 2 Porcentagem De Controle De C Canadensis (Esquerdaunclassified

CONTROLE EM PÓS-EMERGÊNCIA DE Conyza canadensis e Conyza bonariensis COM EXTRATOS DE Canavalia ensiformis

2017

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“…This was the hydroalcoholic sample. [11][12][13] Both samples (aqueous and hydroalcoholic) were analyzed directly by HPLC. They were analyzed in triplicate.…”

Section: Extraction Of Samples For Analysis and Chromatographic Condimentioning

confidence: 99%

“…The samples extracted in the Clevenger apparatus were analyzed and the retention times of their peaks were compared with standards of known compounds whose presence in the samples had been previously determined. [13] Standard solutions of these compounds were prepared at various concentrations and determined by HPLC …”

Section: Extraction Of Samples For Analysis and Chromatographic Condimentioning

confidence: 99%

“…[10] This article presents Canavalia ensiformis as a source of allelopathic compounds, popularly known as jackbean. [11][12][13] This species belongs to the legume family Fabaceae and is much cultivated as green cover, as its roots form symbiotic nodules carrying nitrogen-fixing bacteria, eliminating the use of nitrogen fertilizers. Its remarkable hardiness and ability to grow on soils of low fertility leads immediately to soil improvement.…”

Section: Introductionmentioning

confidence: 99%

“…In view of the possibility that this bioherbicide would be used on soybean (Glycine max), the deleterious effects of the extract on transgenic and conventional soybean were also assessed. In addition, four phenolic compounds and alkaloids with allelopathic properties, previously identified in these extracts, [11][12][13] were quantitated by high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the allelopathic effect of leaf and seed extracts ofCanavalia ensiformisas postemergent bioherbicides: A green alternative for sustainable agriculture

Mendes

Rezende

2014

Journal of Environmental Science and Health, Part B

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The herbicidal potential of aqueous extracts prepared from leaves and seeds of Canavalia ensiformis was tested by applying them postemergence to the crop weeds Ipomoea grandifolia and Commelina benghalensis. Considering the possible objective of using this bioherbicide on soybean fields, the extracts were also tested for deleterious effects on both transgenic and conventional soybean (Glycine max). In addition, the concentrations of four phenolic compounds and alkaloids with allelopathic properties in these extracts were estimated by high-performance liquid chromatography. The seed extract at concentrations of 25 g L −1 and 50 g L −1 was the most efficient postemergent bioherbicide. By applying these extracts, the development of the weed seed was progressively hindered until no recovery was observed. The application of 3.5 mL of crude extract on a plant that takes 30 days after germination of the seed to develop is sufficient for full control of the species within 5 days. Since both Ipomoea grandifolia and Commelina benghalensis are broadleaf dicotyledons, it is possible that other species with these characteristics can also be controlled or suffer the same effects under the action of the seed extract, if the same volume and mode of application are used. The selectivity of the treatments with 25 g L −1 and 50 g L −1 was assessed on transgenic and conventional cultivars. The treatments did not visibly affect the soybean cultivars, which developed normally during the observation period that continued up to 15 days after the death of the weeds.

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Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

Silva

Nishida

Calixto

et al. 2023

Biomedical Chromatography

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Polyamines are low molecular weight compounds that are present in all living organisms. They are related to the pathological processes, and have been studied as biomarkers for tumor progression, being analyzed in patients' biological fluids. However, polyamines can undergo degradation in serum samples, depending on storage conditions, which impairs their quantification in these matrices. In this work, capillary electrophoresis using indirect ultraviolet detection has been developed and applied to evaluate the stability of polyamines [cadaverine (Cad), putrescine (Put), spermine (Spm), and spermidine (Spd)] in human serum at different storage temperatures. By using this method, Cad, Put, Spm, and Spd were separated in less than 4 min. The range of the correlation coefficients was 0.993-0.998. The corresponding limits of detection and quantification were as follows (in mg L -1 ): Spm: 0.209 and 0.697; Spd: 0.165 and 0.549; Put: 0.189 and 0.632; Cad: 0.125 and 0.417. Besides, the coefficient of variation was lower than 1% for all analytes and the recovery was 92%-110%. The method was successfully applied for polyamines spiked in human serum samples from healthy people. The results showed that the degradation of polyamines was lower in samples stored in a freezer (À20 C).

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Determination of polyamines in organic extracts from roots ofCanavalia ensiformisby capillary electrophoresis

Cited by 6 publications

References 23 publications

CONTROLE EM PÓS-EMERGÊNCIA DE Conyza canadensis e Conyza bonariensis COM EXTRATOS DE Canavalia ensiformis

CONTROLE EM PÓS-EMERGÊNCIA DE Conyza canadensis e Conyza bonariensis COM EXTRATOS DE Canavalia ensiformis

Assessment of the allelopathic effect of leaf and seed extracts ofCanavalia ensiformisas postemergent bioherbicides: A green alternative for sustainable agriculture

Evaluation of the stability of polyamines in human serum at different storage temperatures using capillary electrophoresis with indirect ultraviolet detection

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