Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide

Recent advances in separation and detection methods for thiol compounds in biological samples

“…4. MIAC [68], MMPB [69] and 3-MB [70] are new maleimide-type reagents for the derivatization of sulfhydryl-containing compounds. Because the reactivity to the SH group is based on the coupling with the maleimide structure, the derivatization conditions are almost comparable for each maleimide reagent.…”

Methodology for a rapid and simultaneous determination of total cysteine, homocysteine, cysteinylglycine and glutathione in plasma by isocratic RP-HPLC

“…The simultaneous determination of the plasma low molecularmass weight thiols concentrations, such as homocysteine (Hcy), cysteine (Cys), cysteinyl-glycine (Cys-Gly), and glutathione (GSH), is of potential interest for the understanding the dynamic relationship among them, as well as for clinical diagnosis and screening of various human disorders [1], including diabetes, cancer, neurodegenerative and cardiovascular diseases (CVD) [2,3]. These aminothiols ( Fig.…”

“…Different types of labeling reagents have been used, including monobromobimane [30,37], ortho-phthaldialdehyde [38,39], N-(1-pyrenil)maleimide [40,41], 9-acetoxy-2-(4-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)phenyl)-3-oxo-3H-naphtho[2,1-b]pyran (ThioGlo 3) [42], N-(2-acridonyl)maleimide [1], ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F) [20,[43][44][45], and 7-fluoro-2,1,3-benzoxadiazole-4-sulfonamide (ABD-F) [46,47]. The halogenobenzofurazans reagents (SBD-F and ABD-F) are the most often used, since they react selectively with thiol compounds (Fig.…”

Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography

“…Biogenic amines are usually determined by separation techniques like thin layer chromatography (TLC) [22][23][24], capillary electrophoresis (CE) [17,[25][26][27][28][29], gas chromatography (GC) [30][31][32][33][34] and High performance liquid chromatography (HPLC) [21,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49]. HPLC is the technique most extensively used due to its high resolution, sensitivity, great versatility, and simple sample treatment.…”

“…Biogenic amines do not exhibit satisfactory absorption at the visible or ultraviolet wavelengths, nor do they exhibit fluorescence. Therefore, many derivatization agents is usually applied for their analysis such as 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) [40], N-(2-acridonyl)-maleimide (MIAC) [48], benzoyl chloride [38], bis [2-ethylhexyl] sulphosuccinate (AOT) [41], dabsyl chloride (Dbs-Cl) [21,36,37], dansyl chloride (Dns-Cl) [43,44,46], 3,5-dinitrobenzoyl chloride (DNBZ-Cl) [35], 9-fluorenylmethyl chloroformate (FMOC) [51], 9-fluorenylmethyloxy carbonyl chloride (Fmoc-Cl) [45], N-(9-fluorenylmethoxycarbonyloxy) succinimide (Fmoc-Osu) [39], N-hydroxysuccinimidyl fluorescein-o-acetate (SIFA) [49], 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimideester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (TMPAB-OSu) [42], and o-phthalaldehyde (OPA) [47]. 4-Chloro-3,5-dinitrobenzotrifluoride (CNBF) is an important fine chemical, which has been known to react with primary or secondary amines in presence of base to produce stable N-substituted-2,6-dinitro-4-(trifluoromethyl)-benzamine derivatives which are satisfactory ultraviolet absorption [50][51][52][53][54].…”