Recent advances in separation and detection methods for thiol compounds in biological samples

Toyo’oka, Toshimasa

doi:10.1016/j.jchromb.2009.03.034

Cited by 156 publications

(110 citation statements)

References 144 publications

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“…Moreover, low concentration of biologically important thiols in real matrices, high polarity, and good solubility in water make their extraction very troublesome [7,8]. Among a variety of assays designed to determination of thiols in biological fluids, most of them depend on derivatization followed by chromatographic separation and ultraviolet [7][8][9] or fluorescence detection [10][11][12][13][14][15]. Most of chromatographic procedures dedicated to hydrophilic thiols measurements exploit reversed phase (RP)-HPLC and buffered eluents containing ion-pairing reagents, such as alkyl sulphonates or trichloroacetic acid [7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

A simple HPLC—UV method for simultaneous determination of cysteine and cysteinylglycine in biological fluids

Głowacki¹,

Stachniuk²,

Borowczyk³

2016

Acta Chromatographica

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Summary.A new and simple method based on high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for the determination of cysteine (Cys) and cysteinylglycine (CysGly) in plasma and urine has been developed. The method involves reduction of disulfide bonds with tris(2-carboxyethyl)phosphine, derivatization of the analytes with 2-chloro-1-methylquinolinium tetrafluoroborate, and separation on Aeris PEPTIDE XB-C18 column (150 mm × 4.6 mm, 3.6 µm, Phenomenex) with UV detection at 355 nm. The calibration lines, obtained with human plasma and urine spiked with CysGly and Cys, were linear in the range of 2.5-50 μmol L −1 and 20-300 μmol L −1 , respectively. The intra-and inter-day precision values of the method, expressed as a relative standard deviation, were 0.25-11.1% and 0.71-12.3%, respectively. The analytical recovery varied from 89.7 to 112.3%. The LOQs for total Cys and CysGly were 1.5 pmol and 2.3 pmol in peak, respectively. The method was successfully applied to samples donated by apparently healthy individuals. Concentrations of Cys and CysGly in human plasma from 18 subjects varied from 141.6 to 217.8 μmol L −1 and from 21.1 to 50.9 μmol L −1 , respectively. Their concentrations in urine samples (n = 14) ranged from 137.3 to 426.8 μmol L −1 and from 1.6 to 4.9 μmol L −1 , respectively.

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Section: Introductionmentioning

confidence: 99%

A simple HPLC—UV method for simultaneous determination of cysteine and cysteinylglycine in biological fluids

Głowacki¹,

Stachniuk²,

Borowczyk³

2016

Acta Chromatographica

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“…For example, liberated lysine was determined after the reaction with 1, 2-diacetylbenzene but the method requires removal of endogenous lysine from the sample 32) . Other methods reported for the colorimetric determination of thiols and disulfides are not suitable for the detection of small amounts of sulfhydryl compounds like DHLA originated from LA in biological samples [33][34] . Garganta and Wolf developed a colorimetric assay for quantifying LA liberated from lipoyl-N-ε-lysine by lipoamidase action using 2,6-dibromoquinone-4-chlorimide.…”

Section: Fig 1 Working Of Hypothesis Of the Metabolic Fate Of Externmentioning

confidence: 99%

Activity assay of Lipoamidase, an expected modulator of metabolic fate of externally administered lipoic acid

Wang

Motafakkerazad

Matsugo

et al. 2011

Inflammation and Regeneration

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α-Lipoic acid (LA) is a cofactor functioning in four multienzymes: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, branched-chain α-keto acid dehydrogenase, and the glycine cleavage enzyme systems. In each enzyme, LA is covalently bound to the ε-amino group of lysine residues. LA has been used as a medicine for treating diabetic condition in Europe and is also currently a popular supplement resource, and thus the metabolic fate after orally intake of LA attracts much attention whether externally administered LA is incorporated into protein bound form because large fraction of administered dose is not fully explained yet. Lipoamidase is the enzyme catalyzing formation and breakage of amide bond between LA and lysine ε-amino groups in the protein and thus is expected to play critical role in LA metabolism and function. In order to know how the enzyme lipoamidase is involved in the fate of LA in the body, a simple assay method is requested for determining lipoamidase activity in tissues. The method using simple HPLC detection of newly synthesized fluorescent substrate, dansyl-α-lipoyllysine, is discussed together with other previously reported methods. Rec.2/26

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“…As can be seen in Table 1 the developed SI methods were fast enabling a sampling rate of 70 h -1 and quite sensitive with the limits of detection being in the ppbrange for all thiols; ii) the second method involved the investigation of the reaction of the selected thiols with monobromobimane (MBB) under SI conditions. Although MBB is a well-known reagent for thiols [2], up to now it was restricted to applications involving pre-column derivatization prior to separation with liquid chromatography and capillary electrophoresis. Despite of its advantages, the high cost of the reagent was exclusionary for on-line FI-based applications.…”

Section: Determination Of Thiols By Si-fluori-metrymentioning

confidence: 99%

“…Apart from the several review articles on the analysis of thiols [1][2][3][4][5] and the many research articles that appear in the literature, the analytical challenge can be even pointed out by a very recent special issue of Journal of Chromatography B (Elsevier) under the characteristic title "Analysis of thiols" (Volume 877, Issue 28, 2009). …”

Section: Introductionmentioning

confidence: 99%

Automated Determination of Pharmaceutically and Biologically Active Thiols by Sequential Injection Analysis: A Review

Tzanavaras

2010

TOCBMJ

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Abstract:The present review article presents and discusses automated sequential injection (SI) based methods for the determination of pharmaceutically and biologically important thiols, namely cysteine, N-acetylcysteine, penicillamine, glutathione and captopril. The review intends to cover a range of ten years of published research on this topic (2000 -2009). The methods are classified according to the detection systems in three categories, namely spectrophotometric, fluorimetric and electroanalytical. The determination principles of the methods are discussed and the main analytical figures of merit are tabulated.

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Recent advances in separation and detection methods for thiol compounds in biological samples

Cited by 156 publications

References 144 publications

A simple HPLC—UV method for simultaneous determination of cysteine and cysteinylglycine in biological fluids

A simple HPLC—UV method for simultaneous determination of cysteine and cysteinylglycine in biological fluids

Activity assay of Lipoamidase, an expected modulator of metabolic fate of externally administered lipoic acid

Automated Determination of Pharmaceutically and Biologically Active Thiols by Sequential Injection Analysis: A Review

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