Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography

Tang, Tao; Shi, Tianyu; Qian, Kun; Li, Pingliang; Li, Jianqiang; Cao, Yongsong

doi:10.1016/j.jchromb.2008.12.064

Cited by 75 publications

(39 citation statements)

References 58 publications

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“…It can react with amines in low concentration to form stable derivatives under alkaline conditions, and the excess reagent is hydrolyzed to the corresponding phenol without any other by-products and interferences Sun et al 2009;Tang et al 2009). The hydrolysis compound can be written as (CNBF)OH.…”

Section: Derivatives and Derivatization Conditionsmentioning

confidence: 99%

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

Hou

Cao

et al. 2011

Food Anal. Methods

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A novel method was studied for determination of 20 free amino acids in asparagus tin by high-performance liquid chromatography with pre-column derivatization. Derivatization of the samples was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF), and the SPE cartridge was used for purification and enrichment of the analytes. The derivatization conditions and the influence of elution composition on the separation were investigated. The reaction of amino acids with CNBF was completed in pH 9.0 borate buffer. The separation of amino acids was achieved at room temperature within 60 min by gradient elution mode with triethylamine in mobile phase, and the flow rate is 0.32 mL min −1 constantly. The method correlation coefficient was from 0.9975 to 1.0000 in concentrations ranging from 20 to 2000 μmol L −1 , except asparagine (from 100 to 10000 μmol L −1 ). The detection limits of amino acids were from 1.2 to 6.0 μmol L −1 , with a signal-to-noise ratio of three times. The calculated recoveries of the proposed method were from 81.4% to 109.4%, and relative standard deviations were 0.48-3.94% in application to the quantitative determination of free amino acids in asparagus tin samples. The present method is reliable and sensitive that allows fast analysis of free amino acids in asparagus tin, which makes it suitable for further study of free amino acids in other asparagus foods.

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Section: Derivatives and Derivatization Conditionsmentioning

confidence: 99%

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

Hou

Cao

et al. 2011

Food Anal. Methods

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“…The CNBF derivatization was rapid at moderate temperature, along with the resultant derivatives being very stable and of high ultraviolet and fluorescent absorption [41][42][43][44][45][46][47]. There were not any multiple derivatives and by-products except CNBF hydrolysis compound in the reaction, while excess reagent and its hydrolysis compound could not disturb the separation.…”

Section: Introductionmentioning

confidence: 96%

High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride

Shi

Tang

Qian

et al. 2009

Analytica Chimica Acta

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“…Biogenic amines are usually determined by thin layer chromatography (TLC) [6,7], capillary electrophoresis (CE) [8][9][10], gas chromatography (GC) [11,12] and liquid chromatography (LC) with ultra-violet (UV) or fluorescence detection [13][14][15][16][17][18][19][20][21][22][23]25]. Among these methods, LC is the most popular and frequently used for the separation and quantification of biogenic amines.…”

Section: Introductionmentioning

confidence: 99%

“…There are many derivatization reagents usually applied for biogenic amines analysis such as bis-3,5-dinitrobenzoyl chloride (DNBZ-Cl) [13], dabsyl chloride (Dbs-Cl) [14], benzoyl chloride [15], 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) [16], [2-ethylhexyl] sulphosuccinate (AOT) [17], dansyl chloride (Dns-Cl) [18], 9-fluorenylmethyl chloroformate (FMOC) [19], o-phthalaldehyde (OPA) [20], N-hydroxysuccinimidyl fluorescein-o-acetate (SIFA) [21], 1,2-naphthoquinone-4-sulfonate (NOS) [22] and 4-chloro-3, 5-dinitrobenzotrifluoride (CNBF) [23]. In spite of the popularity of these pre-column methods, there have also been many reports describing various shortcomings in their applications, such as short detection wavelengths, poor reproducibility and stability, redundant hydrolysates, incomplete reactions and serious interference for the determination of real samples.…”

Section: Introductionmentioning

confidence: 99%

LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification

You

Sun

et al. 2011

Chromatographia

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A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M ? H] ? in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10 lmol L -1 with coefficients of [0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were \0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.

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Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography

Cited by 75 publications

References 58 publications

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride

LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification

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