Determination of phospholipid fatty acid structures and stable carbon isotope compositions of deep-sea sediments of the Northwest Pacific, ODP site 1179

Mills, Christopher T.; Dias, Robert F.; Graham, D.; Mandernack, Kevin W.

doi:10.1016/j.marchem.2005.10.001

Cited by 19 publications

(17 citation statements)

References 62 publications

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“…Branched and unbranched FAs with chain lengths of C 14 to C 26 and up to one double bond were detected. The highest concentrations were found for FAs C 16:0 , C 16:1 , C 18:0 , and C 18:1 which is consistent with the results of intact lipids and previous studies of PLFA in deeply buried sediments (Summit et al, 2000;Cardace et al, 2006;Mills et al, 2006). Notably, long-chain (C 20 to C 26 ) FAs have been found in all samples with relative amounts of up to 50% of total PLFA.…”

Section: Distribution Of Bacterial Ipls In Marine Sedimentssupporting

confidence: 90%

“…Total lipid extracts were separated on a glass column packed with 0.5 g silica gel (Silica Gel 60, 60-200 lm, Roth, Germany) following the procedure described by Mills et al (2006). Two fractions were eluted: apolar F1 (5 ml dichloromethane) and polar F2 (10 ml acetone followed by 5 ml methanol).…”

Section: Preparation and Analysis Of Phospholipid-derived Fatty Acidsmentioning

confidence: 99%

“…PLFA have been validated as markers for live bacteria, since the "parent" phospholipid is degraded by hydrolytic cleavage of the polar head group bond within days to weeks after cell death (White et al, 1979a;Harvey et al, 1986). Several PLFA-based studies focused on marine sediments and studied the in-situ population (e.g., Summit et al, 2000;Cardace et al, 2006;Mills et al, 2006). However, the assignment of PLFAs to organisms is difficult at times as the fatty acids in surface sediments represent a complex mixture of eukaryotic and bacterial sources, often including contamination by wax esters from higher plant material (Eglinton and Hamilton, 1967;Volkman and Johns, 1977) which sometimes are difficult to exclude from analysis even in deeper samples (Aries et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Structural diversity and fate of intact polar lipids in marine sediments

Lipp

Hinrichs

2009

Geochimica et Cosmochimica Acta

179

151

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Section: Distribution Of Bacterial Ipls In Marine Sedimentssupporting

confidence: 90%

Section: Preparation and Analysis Of Phospholipid-derived Fatty Acidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural diversity and fate of intact polar lipids in marine sediments

Lipp

Hinrichs

2009

Geochimica et Cosmochimica Acta

179

151

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“…FAMEs were identified and quantified using an Agilent 6890 gas chromatograph coupled to an Agilent 5973 mass spectrometer. Microbial biomass in sediment was measured by converting picomoles of lipids per gram to viable cells per gram by multiplying bacterial size based conversion factor of 5.9 × 10 4 cells/pmol (Mills et al, 2006).…”

Section: Phospholipid Fatty Acid Analysismentioning

confidence: 99%

Clay Flocculation Effect on Microbial Community Composition in Water and Sediment

Chen

Pan

Shi

et al. 2018

Front. Environ. Sci.

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Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O 2 added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH + 4 ) concentration and increase of nitrate (NO − 3 ) concentration in zeolite and O 2 loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite capping may also help prevent nutrients from being released back into the water thus preventing additional algal blooms. IMPORTANCEDespite the effectiveness of clay flocculation for removing harmful algal blooms as highlighted in numerous studies, the potential ecological impacts on the microbial community have rarely been investigated. Characterization of clay flocculation treated algal bloom water and sediment microbial community provides new insights into the ecological impacts of this algal bloom controlling technology.

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“…Due to d 13 C values of CH 4 that are generally very negative and large carbon isotope fractionation effects associated with methane uptake, the d 13 C PLFA values of methanotrophs are expected to be more negative than those of heterotrophs or autotrophs in most environments (Zyakun et al, 1987;Jahnke et al, 1999;Cifuentes and Salata, 2001). These fractionation effects demonstrated in the laboratory have been exploited to understand microbial carbon cycling pathways in a variety of environments (Boetius et al, 2000;Orphan et al, 2001;Michaelis et al, 2002;Pearson et al, 2005;Zhang et al, 2005;Mills et al, 2006).…”

Section: As Electron Acceptorsmentioning

confidence: 99%