Microbial carbon cycling in oligotrophic regional aquifers near the Tono Uranium Mine, Japan as inferred from δ13C and Δ14C values of in situ phospholipid fatty acids and carbon sources

Mills, Christopher T.; Amano, Yuki; Slater, G. F.; Dias, Robert F.; Iwatsuki, Teruki; Mandernack, Kevin W.

doi:10.1016/j.gca.2010.03.016

Cited by 43 publications

(37 citation statements)

References 111 publications

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“…For any environment, there can be overlapping ranges of d 13 C biomass values that result from autotrophy and heterotrophy (Mills et al, 2010). Furthermore, isotopic fractionation of assimilated carbon can also occur during lipid synthesis, often resulting in membrane-derived PLFAs that are depleted in 13 C relative to the total biomass by as much as À15& (Zhang et al, 2003).…”

Section: Metabolic Pathwaysmentioning

confidence: 99%

Bacterial abundance and composition in marine sediments beneath the Ross Ice Shelf, Antarctica

et al. 2013

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Marine sediments of the Ross Sea, Antarctica, harbor microbial communities that play a significant role in the decomposition, mineralization, and recycling of organic carbon (OC). In this study, the cell densities within a 153-cm sediment core from the Ross Sea were estimated based on microbial phospholipid fatty acid (PLFA) concentrations and acridine orange direct cell counts. The resulting densities were as high as 1.7 × 10⁷ cells mL⁻¹ in the top ten centimeters of sediments. These densities are lower than those calculated for most near-shore sites but consistent with deep-sea locations with comparable sedimentation rates. The δ¹³C measurements of PLFAs and sedimentary and dissolved carbon sources, in combination with ribosomal RNA (SSU rRNA) gene pyrosequencing, were used to infer microbial metabolic pathways. The δ¹³C values of dissolved inorganic carbon (DIC) in porewaters ranged downcore from -2.5‰ to -3.7‰, while δ¹³C values for the corresponding sedimentary particulate OC (POC) varied from -26.2‰ to -23.1‰. The δ¹³C values of PLFAs ranged between -29‰ and -35‰ throughout the sediment core, consistent with a microbial community dominated by heterotrophs. The SSU rRNA gene pyrosequencing revealed that members of this microbial community were dominated by β-, δ-, and γ-Proteobacteria, Actinobacteria, Chloroflexi and Bacteroidetes. Among the sequenced organisms, many appear to be related to known heterotrophs that utilize OC sources such as amino acids, oligosaccharides, and lactose, consistent with our interpretation from δ¹³CPLFA analysis. Integrating phospholipids analyses with porewater chemistry, δ¹³CDIC and δ¹³CPOC values and SSU rRNA gene sequences provides a more comprehensive understanding of microbial communities and carbon cycling in marine sediments, including those of this unique ice shelf environment.

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Section: Metabolic Pathwaysmentioning

confidence: 99%

Bacterial abundance and composition in marine sediments beneath the Ross Ice Shelf, Antarctica

et al. 2013

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“…The percentage of signature PLFAs 18:3x3 and 20:3x3 showed that cyanobacteria made up the highest fraction of the microbial community as their contribution in PLFA profiles constituted from 28.7 to 81 % of the total PLFAs. The fact that specific lipids and/or fatty acids reflect the taxonomic or functional groups of micro-organisms has been used to detect ammonia-oxidising (AOB) (Saenz et al 2012), sulphate-reducing bacteria (SRB) (Elvert et al 2003) and methanotrophs in sediments (Costello et al 2002;Mills et al 2010). For example, Saenz et al (2012) investigated the presence of anaerobic AOB in the Waquoit Bay subterranean estuary using two types of ladderane lipids.…”

Section: Microbial Diversity In Sedimentsmentioning

confidence: 99%

“…Interestingly, FAME profiles contained a high amount of cyclic unusual fatty acid 17:0cy x5,6 that was proposed by authors as chemotaxonomic markers of the AOM-dependent Desulfosarcina/Desulfococcus species. An assessment of the natural abundance of d 13 C and 14 C values of microbial membrane PLFAs allowed Mills et al (2010) to detect methanotrophs type II in the sediments and granitic groundwater in Japan. The analysis of PLFA profiles showed that 18:1x8c, a biomarker for this microbial group, comprised 3 and 18 % of the total PLFAs in anoxic sediments and granitic waters, respectively.…”

Section: Microbial Diversity In Sedimentsmentioning

confidence: 99%

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

Mrozik

Nowak

Piotrowska‐Seget

2013

Int. J. Environ. Sci. Technol.

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The review summarises recent advances towards a greater comprehensive assessment of microbial diversity in aquatic environments using the fatty acid methyl esters and phospholipid fatty acids approaches. These methods are commonly used in microbial ecology because they do not require the culturing of micro-organisms, are quantitative and reproducible and provide valuable information regarding the structure of entire microbial communities. Because some fatty acids are associated with taxonomic and functional groups of micro-organisms, they allow particular groups of micro-organisms to be distinguished. The integration of fatty acid-based methods with stable isotopes, RNA and DNA analyses enhances our knowledge of the role of micro-organisms in global nutrient cycles, functional activity and phylogenetic lineages within microbial communities. Additionally, the analysis of fatty acid profiles enables the shifts in the microbial diversity in pristine and contaminated environments to be monitored. The main objective of this review is to present the use of lipid-based approaches for the characterisation of microbial communities in water columns, sediments and biomats.

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“…The results of our experiment support this hypothesis. Furthermore, d 13 C and 14 C measurements of bacterial phospholipids from groundwaters of the Toki Lignite-bearing formation indicated heterotrophy as the dominant form of metabolism, including utilization of 14 C dead organic matter as would be expected from lignite degradation (Mills et al 2010).…”

Section: Microbial Degradation Of Organic Matter In the Lignite Layersmentioning

confidence: 95%

Redox Buffer Capacity in Water-Rock-Microbe Interaction Systems in Subsurface Environments

Amano

Sasao

Niizato

et al. 2012

Geomicrobiology Journal

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An incubation experiment was conducted to estimate redox buffer capacity of "water-rock-microbe" interaction systems in sedimentary rocks. The water chemistry, microbial growth and community structure were analyzed during the incubations. The dissolved oxygen (DO) concentrations and oxidation-reduction potential (ORP) values decreased notably in the presence of active microorganisms, whereas abiotic reactions did not lead to reducing conditions during incubation. The change in microbial community structure suggests that nitrate-reducing and sulfate-reducing bacteria played an important role in reduction of water by using lignite-derived organic matter. These results show that the microbial role is extremely important for the redox buffering capacity in sedimentary rock environments.Keywords redox buffering capacity, deep geological disposal of high-level radioactive waste, water-rock-microbe interaction systems, lignite, microbial degradation of high molecular weight organic matter INTRODUCTIONHigh-level radioactive waste (HLW) from nuclear power generation must be isolated in a safe manner. Therefore, many countries have proposed the disposal of HLW deep in geological formations. A reducing environment in the subsurface is a

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Microbial carbon cycling in oligotrophic regional aquifers near the Tono Uranium Mine, Japan as inferred from δ13C and Δ14C values of in situ phospholipid fatty acids and carbon sources

Cited by 43 publications

References 111 publications

Bacterial abundance and composition in marine sediments beneath the Ross Ice Shelf, Antarctica

Bacterial abundance and composition in marine sediments beneath the Ross Ice Shelf, Antarctica

Microbial diversity in waters, sediments and microbial mats evaluated using fatty acid-based methods

Redox Buffer Capacity in Water-Rock-Microbe Interaction Systems in Subsurface Environments

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