Determination of phenylethanolamine A in animal hair, tissues and feeds by reversed phase liquid chromatography tandem mass spectrometry with QuEChERS

Zhang, Mingxia; Li, Cun; Wu, Yuan-li

doi:10.1016/j.jchromb.2012.05.030

Cited by 24 publications

(12 citation statements)

References 16 publications

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“…The performances of the developed method for the analysis of β‐agonists in animal feeds were compared to those of other reports as shown in Table S3. In this work the extraction solvent volume was smaller than almost all other reports and the extraction time (10 min without a pump) was shorter than most other reports . In addition it also provided better recoveries than some methods as well as lower RSDs.…”

Section: Resultsmentioning

confidence: 72%

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

et al. 2015

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A new mixed-mode cation-exchange sulfonate composite functionalized with multiwalled carbon nanotubes with polyvinyl alcohol cryogel was fabricated and used for the first time as a solid-phase extraction sorbent for the determination of β-agonists in animal feeds. Feed samples were extracted with 0.20 M phosphoric acid and methanol (1:4, v/v) using ultrasonication, cleaned-up using the developed sorbent to which the β-agonists bound then finally eluted with 5.0% ammonia in methanol and analyzed by high-performance liquid chromatography. Various parameters that affected the extraction efficiency were optimized. Under the optimal conditions, the developed sorbent strongly interacted with β-agonists by cationic exchange and hydrophobic and hydrophilic interactions, that provided a high extraction efficiency in the range of 92.8 ± 3.7-104.4 ± 2.3% over a range of 0.04-2.0 mg/kg for salbutamol and ractopamine, and 0.40-8.0 mg/kg for clenbuterol. The relative standard deviations were less than 6.0%. The developed method was successfully applied for the determination of β-agonists in various types of animal feed and effectively reduced any matrix interference.

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Section: Resultsmentioning

confidence: 72%

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

et al. 2015

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“…For the detection of PA, the Ministry of Agriculture of China issued a standard analytical method using HPLC‐MS/MS . Recently, there was a report of the LC‐MS/MS for the detection PA in animal hair, tissues and feeds . Generally, chromatographic methods are accurate; however, they are high cost and time‐consuming because of expensive instrument and extensive sample preparation.…”

Section: Introductionmentioning

confidence: 99%

“…18 Recently, there was a report of the LC-MS/MS for the detection PA in animal hair, tissues and feeds. 19 Generally, chromatographic methods are accurate; however, they are high cost and time-consuming because of expensive instrument and extensive sample preparation. Immunoassays are analytical methods which are based on the specific interaction between an antibody and corresponding antigen.…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

et al. 2016

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“…An LC‐MS/MS method is developed to detect PEAA in swine muscle (Sun and Yan ). A UHPLC‐ESI‐MS/MS with QuEChERS method is developed to detect PEAA in animal hair, tissues, and feed (Zhang and others ). A method of direct competitive enzyme‐linked immunosorbent assay (ELISA) is reported to detect PEAA residues in urine samples (Bai and others ).…”

Section: Introductionmentioning

confidence: 99%

Development of an Ultrasensitive Immunochromatographic Assay (ICA) Strip for the Rapid Detection of Phenylethanolamine A in Urine and Pork Samples

et al. 2015

Journal of Food Science

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In this study a one-step immunochromatographic assay based on competitive format was developed for the rapid detection of phenylethanolamine A (PEAA) residues in urine and pork samples. A monoclonal antibody against PEAA was produced from BALB/c mice immunized with the PEAA-BSA conjugate. The results of this qualitative test strip were to be interpreted visually. The visual detection limit (VDL) and threshold level of the optimized immunochromatographic assay for PEAA were 0.1 ng/mL and 0.5 ng/mL, respectively. Cross-reactions with other β-agonists were not significant inhibitions to the performance of the test strip assay. The results from the test strip were in a good agreement with those obtained using a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. The immunochromatographic assay developed here was a useful on-site screening tool that is rapid to use, low in cost, and extremely convenient for the detection of PEAA in urine samples and pork samples.

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Determination of phenylethanolamine A in animal hair, tissues and feeds by reversed phase liquid chromatography tandem mass spectrometry with QuEChERS

Cited by 24 publications

References 16 publications

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

New sulfonate composite functionalized with multiwalled carbon nanotubes with cryogel solid-phase extraction sorbent for the determination of β-agonists in animal feeds

Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

Development of an Ultrasensitive Immunochromatographic Assay (ICA) Strip for the Rapid Detection of Phenylethanolamine A in Urine and Pork Samples

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