Determination of Partition Coefficients of Carotenoids as Tool in Pigment Analysis

Petracek, F. J.; Ƶechmeister, L.

doi:10.1021/ac60117a041

Cited by 137 publications

(37 citation statements)

References 3 publications

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“…The partition coefficients of the acanthizid carotenoid were 17:83 between aqueous 95% methanol and light petroleum, and 42:58 between aqueous 80% methanol and light petroleum. These compare respectively with 12:88 and 4357 for lutein between the methanol solutions and hexane (Petracek & Zechmeister 1956;Foppen 197 1). Variation in the amount of yellow carotenoid with latitude revealed a cline, apparently with increased variability in the region of 28"s (Fig.…”

Section: Resultsmentioning

confidence: 60%

“…The carotenoid was transferred from methanol by adding petroleum spirit and deionized water, and gentle shaking. The spectrum of a neutralised methanolic solution was also recorded to eliminate the possibility of the presence of Partition coefficients provide another common technique for identifying carotenoids (Petracek & Zechmeister 1956;Foppen 197 1). These were determined by extracting the feathers with 5% KOH in methanol, aqueous 95% methanol and in aqueous 80% methanol, adding equal volumes of light petroleum to the filtered extracts, equilibrating, and measuring the absorbance of each phase at the wavelength of maximum absorbance.…”

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confidence: 99%

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Pigment and Morphometric Variation in the Buff-rumped Thornbill

Ford¹,

Simpson²

1987

Emu - Austral Ornithology

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Section: Resultsmentioning

confidence: 60%

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confidence: 99%

Pigment and Morphometric Variation in the Buff-rumped Thornbill

Ford¹,

Simpson²

1987

Emu - Austral Ornithology

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“…Extinction coefficients of 2200 (canthaxanthin) and 2900 (e-carotene) were used (Foppen, 1971). Partition coefficients were determined by the method of Petracek & Zechmeister (1956) and the types and numbers of polar side groups (Mso) estimated by the method of Krinsky (1963). Infrared absorption spectra for structural studies on individual fractions were made on a Perkin-Elmer Model 257 Grating IR recording spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Pigments of a color polymorphism in a cichlid fish

Webber¹,

Barlow²,

Brush³

1973

Comparative Biochemistry and Physiology Part B: Comparative Bio

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Abstract-i. Cichlasoma citrinellum occurs as gray or gold morphs in lakes in Nicaragua. The gold morph is variable and individuals range from yellow to dark orange.2. The pigments responsible for the carotenoid coloration are E-carotene and canthaxanthin. The gray morph contains a carotenoid component and also heavy melanin deposits.3. The carotenoids are dietary in origin, but their distribution and intensity is presumably under genetic control.4. The relation of color morphs to the ecological and selective forces which might produce them is discussed.

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“…The pigments were identified by the following methods: behaviour on column chromatography; absorption spectrum; comparison of Rf values by means of thin-layer chromatography; partition coefficient (Petracek and Zechmeister, 1956;Foppen, 1971). Quantitative determinations of the various carotenoids were made by the method of Davies (1965).…”

Section: Methodsmentioning

confidence: 99%

Carotenoids inNiphargus casimiriensisSkalski (Amphipoda) from artesian wells

Czeczuga¹,

Skalski²

1978

IJS

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Carotenoids are some of the commonest pigments found in nature. They are produced only by plants, both autotrophic and heterotrophic, whereas animals obtain them through the food they eat. These pigments may, however, in the latter undergo further conversion as a result, for example, of oxidation.Various functions have been ascribed to carotenoids ranging from their participation in the photosynthetic process in plants to a role played by these pigments in protecting fruit and invertebrate animals, plankton crustaceans in particular, from the effect and photooxidation of light rays. With the latter function in view, we were interested in the question as to whether carotenoids were present in crustaceans inhabiting subterranean waters where, naturally, the light factor does not play the important role it plays in water exposed to light. In order to study the problem, the carotenoid content of Niphargus casimiriensis Skalski, a species which is to be found in Poland in the artesian wells of Lower Kazimierz on Vistula, was investigated (Skalski, 1976). MATERIAL AND METHODSThe Niphargus casimiriensis specimens were collected from an artesian well in Lower Kazimierz on Vistula. On being removed from the well, the crustaceans were washed thoroughly in distilled water, dried on tissue paper, placed in bottles of dark glass and then covered with acetone. The material was then kept in a refrigerator until removed for the separation of the pigments. Columnar and thin-layer chromatographic methods were used for separation of the carotonoids.Column and thin-layer chromatography were used in the investigations, the method employed was as described in a previous paper (Czeczuga, 1971). Separation of the carotenoids was begun, after preliminary analysis, by

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Determination of Partition Coefficients of Carotenoids as Tool in Pigment Analysis

Cited by 137 publications

References 3 publications

Pigment and Morphometric Variation in the Buff-rumped Thornbill

Pigment and Morphometric Variation in the Buff-rumped Thornbill

Pigments of a color polymorphism in a cichlid fish

Carotenoids inNiphargus casimiriensisSkalski (Amphipoda) from artesian wells

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