Determination of paracetamol, salicylic acid and acetyl salicylic acid in serum by high-performance liquid chromatography

Tebbett, Ian R.; Omile, C. I.; Danesh, B. J. Z.

doi:10.1016/s0021-9673(01)81918-x

Cited by 22 publications

(2 citation statements)

References 4 publications

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“…Three extraction solvents were used in order to compare the extraction efficiency of triflusal and HTB from plasma. Solvent A was acetonitrile-chloroform (40:60, v/v) [5], solvent B was diethylether [2] and solvent C was tert-butyl methyl ether (TBME) [6]. For a human plasma sample (0.5 ml), an aliquot of 100 l of the IS (1 g/ml of furosemide) and 200 l of 0.1 M hydrochloric acid were immediately added and mixed with vortex-mixer for 30 s. For a rat plasma sample (0.1 ml), an aliquot of 100 l of the IS (0.5 g/ml of furosemide) and 100 l of 0.1 M hydrochloric acid were used and mixed.…”

Section: Extraction Proceduresmentioning

confidence: 99%

“…Mobile phase A consisted of a mixture of 50% methanol and 0.005 M dilution of n-tetrabutyl ammonium phosphate (PIC A, Waters, Millipore, Milford, MA, USA) [2][3][4]. Mobile phase B was consisted of acetonitrile-methanol-water (25:10:65, v/v/v) adjusted to pH 2.5 with 85% (w/v) orthophosphoric acid [5]. All separations were performed isocratically at a flow-rate of 1.0 ml/min.…”

Section: Chromatographymentioning

confidence: 99%

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Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Cho

Jeong

Lee

2003

Journal of Chromatography B

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Section: Extraction Proceduresmentioning

confidence: 99%

Section: Chromatographymentioning

confidence: 99%

Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Cho

Jeong

Lee

2003

Journal of Chromatography B

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Review of HPLC methods and HPLC methods with mass spectrometric detection for direct determination of aspirin with its metabolite(s) in various biological matrices

Mullangi

Sharma

Srinivas³

2012

Biomedical Chromatography

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Aspirin, the most widely used drug in the world, has been known to mankind for over a century. It is not only the pharmacologically active entity, but is also biotransformed into a major metabolite, i.e. salicylic acid, which also exhibits similar pharmacologic/pharmacodynamic properties. Hence it is necessary to quantitate aspirin along with its metabolite(s) in various biological matrices accurately and precisely to correlate with pharmacological/pharmacodynamic activity. This paper provides a comprehensive overview of various bioanalytical methods (HPLC and LC-MS/MS) that have been reported for direct quantitation of aspirin along with its metabolite(s). The review also provides general information on sample collection, sample processing, internal standard selection, conditions for chromatographic separation, succinct validation data and applicable conclusions for reported assays in a structured manner.

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Comparative antidotal efficacy of activated charcoal tablets, capsules and suspension in healthy volunteers

Remmert

Olling

Slob

et al. 1990

Eur J Clin Pharmacol

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The efficacy of several formulations of activated charcoal (AC) was compared by measuring the intestinal absorption of a solution of 1 g paracetamol administered 2 min before administration of 5 g AC as suspension (200 ml), tablets (40 of 125 mg) or capsules (25 of 200 mg). The suspension medium without AC was used as the control treatment. Based on the results of a pilot experiment, an 8 subject panel was used in a two 4 x 4 Latin square design. All treatments with AC resulted in a statistically significant decrease in paracetamol absorption compared to the control treatment. The suspension was considerably and significantly more effective than the tablets or capsules. Treatment with tablets was slightly but significantly more effective than capsules. The intake of large numbers of tablets and capsules was difficult. In the hospital AC suspensions are available. For first aid elsewhere, at home, at the working place or in the general practitioner's surgery a preservable and easily redispersible AC formulation would be preferable to the present solid forms.

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Determination of paracetamol, salicylic acid and acetyl salicylic acid in serum by high-performance liquid chromatography

Cited by 22 publications

References 4 publications

Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Review of HPLC methods and HPLC methods with mass spectrometric detection for direct determination of aspirin with its metabolite(s) in various biological matrices

Comparative antidotal efficacy of activated charcoal tablets, capsules and suspension in healthy volunteers

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