Review of HPLC methods and HPLC methods with mass spectrometric detection for direct determination of aspirin with its metabolite(s) in various biological matrices

Abstract

Aspirin, the most widely used drug in the world, has been known to mankind for over a century. It is not only the pharmacologically active entity, but is also biotransformed into a major metabolite, i.e. salicylic acid, which also exhibits similar pharmacologic/pharmacodynamic properties. Hence it is necessary to quantitate aspirin along with its metabolite(s) in various biological matrices accurately and precisely to correlate with pharmacological/pharmacodynamic activity. This paper provides a comprehensive overview of various bioanalytical methods (HPLC and LC-MS/MS) that have been reported for direct quantitation of aspirin along with its metabolite(s). The review also provides general information on sample collection, sample processing, internal standard selection, conditions for chromatographic separation, succinct validation data and applicable conclusions for reported assays in a structured manner.

Absorption kinetics of low‐dose chewable aspirin – implications for acute coronary syndromes

“…Acetylsalicylic acid is the acetylated form of salicylic acid with a pKa of 3·5 . Following administration, acetylsalicylic acid is rapidly hydrolysed by ubiquitous esterases to salicylic acid, a weak acid which is the main metabolite .…”

Poly[(2‐(acryloyloxy) ethyl]trimethylammonium chloride‐co‐ethylene dimethacrylate monolith on‐line solid‐phase extraction coupled with liquid chromatography and tandem mass spectrometry for the fast determination of salicylic acid in foodstuffs

“…SA (and its salicylate anion) is the principal metabolite of ASA and it is the pharmacologically active substance in food and biological samples . Many chromatographic methods including CE , LC–UV , and LC–MS/MS have been proposed for the analysis of SA in food and biological samples.…”

Separation of Aliphatic and Aromatic Carboxylic Acids by Conventional and Ultra High-Performance Ion-Exclusion Chromatography

“…These methods include GC [18], reversed phase LC [19, 20], LC/MS [21], CE [22], microdialysis [23] and voltammetry [24]. These reversed phase LC methods used conventional C18 columns with acidic (pH 2.5) mobile phases to keep the aromatic carboxylic acids neutral in charge.…”