Determination of orotic acid in aqueous solutions by micellar electrokinetic capillary chromatography using sensitized lanthanide-ion luminescence detection

Milofsky, Robert E.; Spaeth, S.

doi:10.1007/bf02271048

Cited by 16 publications

(14 citation statements)

References 27 publications

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“…In CE, terbium sensitized luminescence has been used for the detection of steroids 24 and for orotic acid. 25 In these applications, terbium salt had been added to the separation buffer.…”

mentioning

confidence: 99%

Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

1997

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A method has been developed for the determination of catecholamines and related compounds (DA, E, NE, DOPA, DOPAC, DOPAG) in urine samples. The compounds of interest were separated by capillary electrophoresis with a borate buffer as background electrolyte. Detection was based on the sensitized luminescence of terbium ions. Using a homemade postcolumn reactor, a basic solution containing a stoichiometric mixture of terbium(III) chloride and EDTA was added postcolumn to the separation buffer. The ternary catechol−EDTA−terbium complexes formed in the reaction mixture showed a strong luminescence with excitation and emission maxima at 300 and 545 nm, respectively. By optimization of the experimental conditions, zone broadening could be restrained and plate numbers up to 130 000 appeared to be possible. Detection limits found were in the order of 10-7 mol L-1. The catecholic compounds could be determined in urine samples after a standard cleanup and preconcentration procedure.

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“…In CE, terbium sensitized luminescence has been used for the detection of steroids 24 and for orotic acid. 25 In these applications, terbium salt had been added to the separation buffer.…”

mentioning

confidence: 99%

Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

1997

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“…The system was found to be stable for several weeks provided that the capillaries were rinsed and stored with deionized water overnight. Moreover, column conditioning only required a 2-minute flush with the running buffer, a significant improvement over earlier methods were terbium or europium was added to the running buffer [28][29]351. Figure 2 illustrates a typical run employing post-column addition of terbium followed by sensitized terbium luminescence detection.…”

Section: Resultsmentioning

confidence: 99%

“…The Iuminescence intensity is further increased by the addition of the surfactant CTAB to alkaline diflunisal and salicylic acid solutions. Addition of this cationic surfactant most likely increases luminescence by increasing the efficiency of the energy transfer process by compartmentalization and reducing the amount of quenching from water [29,341. Although static studies showed that the optimum CTAB concentration was 5 x M (pH = 12), the combination of a high surfactant concentration under alkaline conditions in the presence of Tb3+ led to the formation of a white precipitate.…”

Section: Resultsmentioning

confidence: 99%

“…In HPLC, lanthanide ions have been added post-column while in CE, terbium and europium have been added to the running buffer [9,[28][29][30]351. The application of sensitized lanthanide luminescence detection in CE is complicated by the strong adsorption of terbium and europium to the negatively charged walls of the silica capillaries as well as the formation of metal-ligand complexes with differing coordination numbers.…”

Section: Resultsmentioning

confidence: 99%

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Capillary electrophoresis with post‐column addition of terbium and sensitized lanthanide‐ion luminescence detection for the determination of diflunisal and salicylic acid

Milofsky¹,

Bauer²

1997

J. High Resol. Chromatogr.

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SummaryA detection scheme based on the post-column addition of terbium followed by sensitized luminescence detection has been developed.Two model compounds -salicylic acid and diflunisal -were separated by capillary electrophoresis in an alkaline phosphate buffer. A solution containing terbium and CTAB was added post-column via a "home-built" reactor resulting in the rapid formation of the strongly luminescent terbium-salicylic acid and terbium-diflunisal complexes in a reaction capillary. Following complexation in the reaction capillary, the 325 nm line from a HeCd laser was used to excite the analytes which efficiently transfer their energy to terbium. Resultant luminescence of terbium was linear of 2 orders of magnitude. This novel and practical system enables the detection of micromolar concentrations of diflunisal and salicylic acid -a 10-fold improvement over UV absorbance detection in the same system. Post-column addition of terbium also eliminates the problems associated with strong adsorption of the lanthanide ion to the silica walls of the separation capillary and the formation of metal-ligand complexes with different coordination numbers.

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“…The first description on the use of lanthanide ions as luminescent detection chromophores in liquid chromatography was by DiBella et al [39] for the detection of aromatic ketones and aldehydes. Since then, the method has been extended to a number of other compounds including tetracyclines [40,41], single-stranded nucleic acids [42], nalidixic acid and bleomycins [43], indoles and heterocyclics [44], orotic acid [45,46], steroids such as progesterone and testosterone [47,48], diphacinone [49], fluoroquinolines [50], propyl gallate [51], ochratoxin A and citrinin [52], theopylline [53], and hydrolysis products of dexrazoxane [54]. Inorganic ions have been measured using quenched lanthanide luminescence detection in electrophoretic techniques has also been described [46,48,55].…”

Section: Time-resolved Fluorometric Hplcmentioning

confidence: 99%

Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses

Matsuya¹,

Hoshino²,

Okuyama

2006

CAC

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Lanthanide complexes have been used for fluorescent labels to the wide variety of analytical methods including high performance liquid chromatography, immunoassay, and fluorescent imaging with time-resolved fluorometric measurements. Introduction of a time-resolved fluorometric measurement has been permitted the easy distinction of a specific long-lived fluorescence signal from short-lived natural background fluorescence present in most biological samples, reaction wells and optical components. Recently, new fluorescent labels and new instruments have been reported for timeresolved fluorescence including high performance liquid chromatography as in environmental analysis as river water, clinical chemistry as allergen assay, and a biochemical analysis as in staining method of proteins on polyacrylamide gel. The time-resolved fluorometric detection methods using new fluorescent labels improved the detection sensitivity compared to those of previously-described methods. This paper reviews those developments of applications of lanthanide fluorescent labels for various purposes: time-resolved fluorometric high performance liquid chromatography, timeresolved fluorescent immunoassay, and protein staining in recent years.

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Determination of orotic acid in aqueous solutions by micellar electrokinetic capillary chromatography using sensitized lanthanide-ion luminescence detection

Cited by 16 publications

References 27 publications

Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

Capillary electrophoresis with post‐column addition of terbium and sensitized lanthanide‐ion luminescence detection for the determination of diflunisal and salicylic acid

Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses

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