Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

Zhu, Rong; Kok, Wim Th.

doi:10.1021/ac970323m

Cited by 122 publications

(71 citation statements)

References 29 publications

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“…Various approaches for Ep analysis include methods such as fluorescence, [7][8][9][10] chemiluminescence, 11 radioimmunoassay, 12 capillary electrophoresis [13][14][15] and liquid chromatography coupled with several types of detection methods. 16,17 The detection of tyrosine is usually accomplished by spectrophotometric, 18 fluorimetric, 19 and liquid chromatography-tandem mass spectrometry, 20 gas chromatography-mass spectrometry, 21 and highperformance liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 The absence of Tyr could cause albinism and alkaptonuria, while a high L-tyrosine concentration in culture medium results in increased sister chromatid exchange. Thus, the analytical determination of Tyr is a pharmacological necessity.Various approaches for Ep analysis include methods such as fluorescence, 7-10 chemiluminescence, 11 radioimmunoassay, 12 capillary electrophoresis [13][14][15] and liquid chromatography coupled with several types of detection methods. 16,17 The detection of tyrosine is usually accomplished by spectrophotometric, 18 fluorimetric, 19 and liquid chromatography-tandem mass spectrometry, 20 gas chromatography-mass spectrometry, 21 and highperformance liquid chromatography.…”

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confidence: 99%

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A sensitive simultaneous determination of epinephrine and tyrosine using an iron(III) doped zeolite-modified carbon paste electrode

Babaei¹,

Mirzakhani²,

Khalilzadeh³

2009

J. Braz. Chem. Soc.

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Um eletrodo de pasta de carbono modificado com zeólitas e dopado com ferro(III) foi usado como um sensor eletroquímico de alta sensibilidade e seletividade para a determinação simultânea de pequenas quantidades de compostos biologicamente importantes como epinefrina (Ep) e tirosina (Tyr). Os resultados da voltametria de pulso diferencial (DPV) usando este eletrodo modificado mostra duas ondas anódicas bem traçadas para a oxidação da Ep e da Tyr, as quais tornam possíveis a determinação simultanêa destes compostos. A faixa linear de 0,9 a 216 mmol L -1 para a Ep em uma concentração fixa de 30 mmol L -1 Tyr em solução tampão fosfato, pH 3,0 foi obtida das medidas de DPV usando este eletrodo, com um coeficiente de correlação de 0,9998 e limite de quantificação igual a 0,44 mmol L -1 . A linearidade obtida para a Tyr na presença de 30 mmol L -1 de Ep, foi de 1,2 a 90 mmol L -1 com um coeficiente de correlação de 0,9989 e limite de quantificação de 0,32 mmol L -1 . O eletrodo modificado apresentou uma boa reprodutibilidade (RSD< 2.5%), baixo limite de quantificação (sub-micromolar) e alta sensibilidade para a detecção de ambos Ep e Tyr com uma elevada estabilidade em sua resposta voltamétrica. O desempenho analítico deste sensor tem sido avaliado para a detecção de epinefrina e tirosina em soro humano e em amostras farmacêuticas com resultados satisfatórios.A Zeolite modified carbon paste electrode doped with iron(III) was used as a highly sensitive and selective electrochemical sensor for simultaneous determination of minor amounts of epinephrine (Ep) and tyrosine (Tyr) as the biologically important compounds. The results of differential pulse voltammetry (DPV) using this modified electrode show two well-resolved anodic waves for the oxidation of Ep and Tyr ,which makes it possible for simultaneous determination of both compounds. A linear range from 0.9 to 216 mmol L -1 for Ep at a constant concentration of 30 mmol L -1 Tyr in buffered solution (phosphate buffer, pH 3.0) was obtained from DPV measurement using this electrode with a correlation coefficient of 0.9998 and a detection limit of 0.44 mmol L -1 . The linear range, which is obtained for Tyr in the presence of 30 mmol L -1 Ep, was in the range from 1.2 to 90 µmol L -1 with a correlation coefficient of 0.9989 and a detection limit of 0.32 mmol L -1 . The modified electrode had good reproducibility (RSD < 2.5%), low detection limit (sub-micromolar) and high sensitivity for the detection of both Ep and Tyr with a very high stability in its voltammetric response. The analytical performance of this sensor has been evaluated for detection of Ep and Tyr in human serum and in a pharmaceutical sample with satisfactory results.Keywords: Epinephrine, tyrosine, Fe(III) doped zeolite, modified carbon paste electrode IntroductionEpinephrine (Ep), Scheme 1 (a), often called adrenaline, is one of the most important neurotransmitters in mammalian central nervous systems, existing in the nervous tissue and body fluid, and controlling the nervous system in the performance of ...

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

A sensitive simultaneous determination of epinephrine and tyrosine using an iron(III) doped zeolite-modified carbon paste electrode

Babaei¹,

Mirzakhani²,

Khalilzadeh³

2009

J. Braz. Chem. Soc.

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“…The determination of EP, a group of catecholamines, is primarily useful for the diagnosis for various diseases and an evaluation of their medical treatments. [13][14][15] We then proposed the Luc CL method for the determination of EP by a conventional batch method.…”

Section: Measurement Of CL Response Curvesmentioning

confidence: 99%

Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip

et al. 2001

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The chemiluminescent (CL) detection of epinephrine (EP) with lucigenin (Luc) was performed using a micro flow cell fabricated on a silicon chip. A solution of EP was injected into the Luc carrier stream. The Luc solution containing EP and an alkaline solution were successively poured into the flow cell by a pressure-driven flow system. Two types of flow cells were fabricated for estimating the effect of the mixing modes in the flow cells on the intensity of light emission. In flow cell 1, two streams entered through separate inlet ports and merged to flow adjacently. In flow cell 2, a Luc solution containing EP was split up to 36 partial flows by passage through the nozzles, and was injected into the alkaline solution. The intensity of light emission in flow cell 2 increased markedly compared to that in flow cell 1. The detection limit of 8.0 × 10 -7 M for EP in flow cell 2 was a factor of six-times better than that in flow cell 1. The improvement in the sensitivity for EP could be explained in terms of the distortion of laminar flow in flow cell 2.

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“…Previously, a few analyses of several lanthanide complexes by capillary electrophoresis had been reported, but in those systems the metal ion was complexed with a ligand either on the column with a free ligand added in the carrier buffer to avoid metal dissociation, [24][25][26][27][28][29] or a metal ion was introduced after capillary electrophoresis in order to avoid metal dissociation during electrophoresis. 30 Early in lanthanide chelate research, a Tb chelate complex with a ligand obtained from the reaction of sodium p-aminosalicylate with diethyltriamine pentaacetic acid (DTPA) dianhydride was reported to be a stable Tb chelate label for DNA in gel electrophoresis. 31 The label was stable during electrophoresis and stable on-line monitoring of the Tb luminescence was possible.…”

Section: Introductionmentioning

confidence: 99%

Sodium Dodecyl Sulfate Polyacrylamide Slab Gel Electrophoresis and Hydroxyethyl Cellurose Gel Capillary Electrophoresis of Luminescent Lanthanide Chelate-labeled Proteins with Time-Resolved Detection

et al. 2009

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Proteins labeled with a luminescent lanthanide chelate, {{2,2¢,2≤,2¢¢¢-{4¢-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2¢:6¢,2≤-terpyridine-6,6≤-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTAEu 3+ ), were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) slab gel electrophoresis and hydroxyethyl cellurose gel capillary electrophoresis with a time-resolved fluorometric detector. The metal ion of the luminescent lanthanide chelate did not dissociate from the ligand during electrophoresis, and the luminescence was retained. On a slab gel, the band of DTBTA-Eu 3+ -labeled streptavidin was apparently less broad than that of AlexaFluor 488-labeled streptavidin. DTBTA-Eu 3+ in SDS-PAGE slab gel is stable, and the gel after electrophoresis can be dried and stored for at least one year without luminescence fading. In capillary gel electrophoresis (CGE), five labeled proteins with different molecular weight were separated, and a good correlation was observed between the molecular weight and the migration time. Although the detection limits of these proteins determined in buffer solutions of the capillary electrophoresis were not better than those reported for CGE with laser-induced-detection, the detection limits of the same proteins in the present CGE system were not significantly deteriorated in serum solutions compared to those in buffer solutions, and the advantage of using time-resolved detection has been shown.

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Determination of Catecholamines and Related Compounds by Capillary Electrophoresis with Postcolumn Terbium Complexation and Sensitized Luminescence Detection

Cited by 122 publications

References 29 publications

A sensitive simultaneous determination of epinephrine and tyrosine using an iron(III) doped zeolite-modified carbon paste electrode

A sensitive simultaneous determination of epinephrine and tyrosine using an iron(III) doped zeolite-modified carbon paste electrode

Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip

Sodium Dodecyl Sulfate Polyacrylamide Slab Gel Electrophoresis and Hydroxyethyl Cellurose Gel Capillary Electrophoresis of Luminescent Lanthanide Chelate-labeled Proteins with Time-Resolved Detection

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