Determination of optimum ethylmethanesulfonate conditions for chemical mutagenesis of selected vernonia (<i>Centrapalus pauciflorus</i>) accessions

Hadebe, Sandile T.; Modi, Albert T.; Shimelis, Hussein

doi:10.1080/02571862.2017.1317851

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…Induced mutation offers an opportunity to develop high‐performing and well‐adapted genotypes possessing useful agronomic traits such as seed size, plant height and seed yield as well as resistance/tolerance to abiotic and biotic stresses (Gwata, Shimelis, & Matova, 2016; Kumar, Katna, & Sharma, 2019). Different types of mutagens such as gamma rays and ethyl methane sulphonate (EMS) have been utilized to create genetic variation in cultivated crops (Hadebe, Modi, & Shimelis, 2017; Olaolorun, Shimelis, Mathew, & Laing, 2019). However, ethyl methane sulphonate is preferred as it creates large number of point mutations and its mutation frequency is independent of the genome size (Greene et al., 2003; Henikoff & Comai, 2003; McCallum, Comai, Greene, & Henikoff, 2000).…”

Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

“…This shows that mutation breeding has a potential of creating sufficient genotypic and phenotypic variation for genetic improvement and selection of mutant lines for drought tolerance breeding. However, mutagenesis using EMS is reported to affect germination capacity (Olaolorun et al, 2019) and thus requires a dose formulation specific for each genotype (Gwata et al, 2016; Hadebe et al, 2017; Olaolorun et al, 2019).…”

Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

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Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

et al. 2020

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Tepary bean (Phaseolus acutifolius A. Gray) is a relatively higher drought‐tolerant crop than common bean (P. vulgaris), serving as genetic resource for food and genetic enhancement of related legumes. Tepary bean production is hampered by cultivation of low yielding and abiotic stress‐susceptible cultivars. Targeted selection of agronomic, physiological and biochemical traits that maximizes yield gains using Phaseolus gene pool is useful to develop stress‐tolerant and high‐performing genotypes. The objective of this review is to provide breeding progress made regarding tepary bean improvement for drought adaptation. Agronomic, physiological and biochemical traits utilized for selection of drought‐tolerant genotypes are highlighted. Genetic and genomic resources developed for tepary bean or closely related species such as common bean useful for genetic analysis and breeding are discussed. Opportunities and challenges to facilitate breeding of tepary bean genotypes with improved abiotic stress adaptation are highlighted. This will enable development of drought‐tolerant tepary bean genotypes targeting selection of agronomic, physiological and biochemical traits. Use of genetically related and complementary Phaseolus species and marker‐assisted selection method is key to developing drought‐tolerant genotypes.

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Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

et al. 2020

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“…Then, with a 6-hour immersion period, four concentrations were chosen: 0.05%, 0.06%, 0.07%, and 0.08%. According to the following procedure, the EMS reagent was made by dissolving EMS in distilled water and 2% DMSO (Hadebe et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Induction of Protocorm-Like Bodies (PLBs) Phalaenopsis spp. Hybrids Mutation through Ultraviolet Irradiation (UV254) and Ethyl Methane Sulfonate (EMS)

et al. 2023

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Phalaenopsis sp. is the most-produced orchid species in Indonesia. Compared to conventional breeding, mutation induction by using mutagens, such as Ultraviolet Light-C (λ = 254 nm) (UV254) and Ethyl Methane Sulfonate (EMS), could probably result in new superior orchid variants. This research aims to get some mutants with phenotypes that have visual differences in the Phalaenopsis spp. hybrids wild type. There were 4 durations of UV254 irradiation: 5’ on, 85’ off; 10’ on, 80’ off (1 day and 7 days for each treatment); 4 concentrations of EMS used in this research: 0.05%; 0.06%; 0.07%; 0.08% for 6 hours of immersion; selected UV254 irradiation (5’ on, 85’ off (7 days)) combined with these concentrations. UV254 irradiation treatment (5’ on, 85’ off (1 day and 7 days); 10’ on, 80’ off (7 days)) resulted in some mutants with leaf phenotypes that were visually different from the wild type; 0.05% EMS treatment resulted in PLBs mutant with a visually larger size than the wild type; 0.08% EMS treatment and combination treatments (for EMS 0.05% and 0.08% for each treatment) resulted in non-growing albino PLBs. Hence, mutation induction using UV254 and EMS in this research produced several most likely mutants having visual differences that may be more desirable than the wild type.

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“…De acuerdo a lo mencionado anteriormente también podemos mencionar que las auxinas desempeñan un papel vital en el desarrollo lateral y vertical de las plantas (Hadebe et al, 2017). Como tal, la inactivación de estas enzimas debido a la mutagénesis por EMS podría ser no solo la causa principal de las disminuciones observadas en la altura de las plántulas, sino también la causa de la perdida de dominancia apical en las plántulas.…”

Section: Altura De Planta Y Dominancia Apicalunclassified

Determinación de la DL50 de Metanosulfonato de Etilo (EMS) para la inducción de cambios morfológicos y fisiológicos en plántulas de Plukenetia volubilis

Corazón-Guivin

Rojas²,

Acosta-Córdoba

et al. 2022

Rev. agrotec. amaz

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El uso de mutágenos químicos es una herramienta muy utilizada para la generación de nuevas variantes genéticas en diversos cultivos agrícolas. Se evaluó el uso Ethyl Methanesulphonate (EMS) en semillas de Plukenetia volubilis L. para determinar la concentración óptima de EMS que redujera la germinación y/o emergencia de las semillas hasta un 50.0%, y evaluar las alteraciones morfológicas y fisiológicas en plántulas de P. volubilis durante la primera generación. Se empleó un DCA simple con diferentes dosis (0.0%, 0.5%, 1.0%, 1.5%, 2.0% y 3.0%) de EMS en un solo tiempo de exposición (30 hrs.), más un control absoluto (semillas sin tratamiento), para evaluar la sensibilidad mutagénica de P. volubilis L.., considerando parámetros como porcentaje de emergencia, altura de planta, pérdida de dominancia apical, clorosis y deformación de las hojas. Los resultados mostraron que la dosis de 3.0% de EMS con 30 hrs. de exposición, redujo hasta un 50.0% la emergencia de plántulas, valor considerado como la dosis letal media (DL50) para P. volubilis. Así mismo, se evidenciaron alteraciones fenotípicas como deformación de hojas, clorosis, disminución de la altura y pérdida de dominancia apical con el incremento de dosis de EMS. Estos resultados demuestran el potencial del EMS para ser utilizados en semillas de sacha inchi con el objetivo de generar nuevas variantes genética de esta especie.

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Determination of optimum ethylmethanesulfonate conditions for chemical mutagenesis of selected vernonia (Centrapalus pauciflorus) accessions

Cited by 6 publications

References 18 publications

Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

Induction of Protocorm-Like Bodies (PLBs) Phalaenopsis spp. Hybrids Mutation through Ultraviolet Irradiation (UV254) and Ethyl Methane Sulfonate (EMS)

Determinación de la DL50 de Metanosulfonato de Etilo (EMS) para la inducción de cambios morfológicos y fisiológicos en plántulas de Plukenetia volubilis

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