Determination of local anaesthetics and their impurities in pharmaceutical preparations using HPLC method with amperometric detection

Fijałek, Zbigniew; Baczyński, Emil; Piwońska, Agata; Warowna-Grześkiewicz, Małgorzata

doi:10.1016/j.jpba.2004.09.025

Cited by 36 publications

(37 citation statements)

References 16 publications

(15 reference statements)

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“…Several chromatographic and spectroscopic techniques were developed in order to determine prilocaine concentrations in both pharmaceutical preparations and human plasma [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] .…”

Section: Fig 1: Chemical Structure Of Prilocaine Hclmentioning

confidence: 99%

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2012

IJPSR

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A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical preparation. INTRODUCTION:The reason of using local anesthetics is to block the conduction of impulses in nerve fibers which cause anesthesia. They are most commonly used in dentistry and minor surgery in order to provide temporary relief of pain. There are two application types of local anesthetics which are topical and parenteral applications that reversibly block the nerve conductances.

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Section: Fig 1: Chemical Structure Of Prilocaine Hclmentioning

confidence: 99%

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2012

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“…Tampão a PH 7,9: acetonitrila (45:55)] foi selecionado para a separação e determinação dos anestésicos estudados e suas impurezas. O método desenvolvido neste estudo é sensível e seletivo e pode ser aplicado em estudos de rotina nas formas farmacêuticas de cremes e injetáveis [13].…”

Section: Determinação De Anestésicos Locais E Suas Impurezas Em Prepaunclassified

“…Estas impurezas podem ser formadas durante a síntese destes anestésicos locais ou durante a estocagem das preparações [13].…”

Section: Determinação De Anestésicos Locais E Suas Impurezas Em Prepaunclassified

“…Atualmente, seu emprego em vários laboratórios é considerado indispensável por químicos, farmacêuticos, bioquímicos e outros [13].…”

Section: Introdução Da Técnica De Análiseunclassified

“…È um tipo de cromatografia líquida que emprega colunas recheadas com materiais especialmente preparados e uma fase móvel, eluída sob altas pressões. Ela tem a capacidade de realizar separações e análises quantitativas de uma grande variedade de compostos presentes em diversos tipos de amostras, em escalas de tempo de poucos minutos, com alta resolução, eficiência e detectibilidade [13].…”

Section: Introdução Da Técnica De Análiseunclassified

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Estudo analítico de anestésicos injetáveis e validação de estabilidade

Merheb¹

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Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

Liu

Yang

et al. 2012

Luminescence

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A simple and new method for the simultaneous determination of procaine (Pro), lidocaine (Lid), ropivacaine (Rop) and bupivacaine (Bup) was developed using capillary electrophoresis separation with mixed micelles and electrochemiluminescence detection. The use of mixed micelles of 2.0 × 10(-3) mol/L sodium dodecyl sulfate (SDS) and 8.0 × 10(-3) mol/L Tween 20 greatly improved separation selectivity. The detection sensitivities of four drugs with a Pt working electrode were increased by modification of the Pt electrode with europium(III)-doped Prussian Blue analog (Eu-PB). Under optimal conditions, the four local anesthetics were well separated and detected. The limits of detection (LOD, S/N = 3) of Pro, Lid, Rop and Bup in standard solution are 2.5 × 10(-8) , 1.3 × 10(-8) , 3.0 × 10(-8) and 4.1 × 10(-8) mol/L, respectively. The limits of quantitation (LOQ, S/N = 10) of Pro, Lid, Rop and Bup are 2.3 × 10(-7) , 1.2 × 10(-7) , 3.7 × 10(-7) and 5.6 × 10(-7) mol/L in a human urine sample, and 8.5 × 10(-7) , 6.9 × 10(-7) , 2.8 × 10(-6) and 1.1 × 10(-6) mol/L in a human serum sample, respectively. The recoveries of four drugs at different spiked concentrations in human urine and serum samples were between 86.5 and 107.6%. The proposed method has been successfully applied to determine local anesthetics in biofluids.

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Determination of local anaesthetics and their impurities in pharmaceutical preparations using HPLC method with amperometric detection

Cited by 36 publications

References 16 publications

Untitled

Untitled

Estudo analítico de anestésicos injetáveis e validação de estabilidade

Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

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