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Abstract

A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical preparation. INTRODUCTION:The reason of using local anesthetics is to block the conduction of impulses in nerve fibers which cause anesthesia. They are most commonly used in dentistry and minor surgery in order to provide temporary relief of pain. There are two application types of local anesthetics which are topical and parenteral applications that reversibly block the nerve conductances.