Determination of inorganic phosphate by the molybdovanadate method in the presence of ATP and some interfering organic bases

Ueda, Issaku; Wada, Tomio

doi:10.1016/0003-2697(70)90273-3

Cited by 44 publications

(16 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Samples were centrifuged at 1500×g for 10 min to obtain the final product which was present in the supernatant [19]. Lastly, samples were analyzed for the presence of inorganic phosphate using the Molybdovanadate method [20] and assessed in triplicate at a wavelength of 400 nm using a Synergy 4 microplate reader (Bio-Tek, Winooski, VT). Specific Na + /K + ATPase activity was determined by the difference in inorganic phosphate (P i ) production from ATP in the presence of absence of ouabain (specific Na + /K + ATPase inhibitor).…”

Section: Methodsmentioning

confidence: 99%

Heat Stress Reduces Intestinal Barrier Integrity and Favors Intestinal Glucose Transport in Growing Pigs

et al. 2013

View full text Add to dashboard Cite

Excessive heat exposure reduces intestinal integrity and post-absorptive energetics that can inhibit wellbeing and be fatal. Therefore, our objectives were to examine how acute heat stress (HS) alters intestinal integrity and metabolism in growing pigs. Animals were exposed to either thermal neutral (TN, 21°C; 35–50% humidity; n = 8) or HS conditions (35°C; 24–43% humidity; n = 8) for 24 h. Compared to TN, rectal temperatures in HS pigs increased by 1.6°C and respiration rates by 2-fold (P<0.05). As expected, HS decreased feed intake by 53% (P<0.05) and body weight (P<0.05) compared to TN pigs. Ileum heat shock protein 70 expression increased (P<0.05), while intestinal integrity was compromised in the HS pigs (ileum and colon TER decreased; P<0.05). Furthermore, HS increased serum endotoxin concentrations (P = 0.05). Intestinal permeability was accompanied by an increase in protein expression of myosin light chain kinase (P<0.05) and casein kinase II-α (P = 0.06). Protein expression of tight junction (TJ) proteins in the ileum revealed claudin 3 and occludin expression to be increased overall due to HS (P<0.05), while there were no differences in claudin 1 expression. Intestinal glucose transport and blood glucose were elevated due to HS (P<0.05). This was supported by increased ileum Na+/K+ ATPase activity in HS pigs. SGLT-1 protein expression was unaltered; however, HS increased ileal GLUT-2 protein expression (P = 0.06). Altogether, these data indicate that HS reduce intestinal integrity and increase intestinal stress and glucose transport.

show abstract

Section: Methodsmentioning

confidence: 99%

Heat Stress Reduces Intestinal Barrier Integrity and Favors Intestinal Glucose Transport in Growing Pigs

et al. 2013

View full text Add to dashboard Cite

show abstract

“…12) The hydroxyproline concentration was determined by a colorimetric method, in which the hydrolyzed solution was oxidized with chloramine-T and applied to colorimetry by Ehrlich reaction at 558 nm. 13) The maximum breaking force of the left femoral diaphysis (the center of the femur) was measured as the bone strength. A threepoint bending test 14) was performed with a rheometer (RE-3305 Rheoner; Yamaden, Tokyo) under the following conditions: 1.0 cm of sample space; 30 mm/min of pranger speed; 20 kg of load range.…”

Section: Improvement Of Bone Strength and Dermal Thickness Due To Diementioning

confidence: 99%

Improvement of Bone Strength and Dermal Thickness Due to Dietary Edible Bird’s Nest Extract in Ovariectomized Rats

Matsukawa

Matsumoto

Bukawa³

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…Change in absorbance over time at 340 nm was measured, and NADH generation rate was determined. One unit was defined as the amount of enzyme necessary to produce 1 mmole of NADH per minute at 258C [22]. All the reagents were purchased from Sigma-Aldrich unless otherwise specified.…”

mentioning

confidence: 99%

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Kuchukashvili¹,

Menabde²,

Chachua³

et al. 2011

ABBS

View full text Add to dashboard Cite

We studied the functionality of the antioxidant system in laboratory rat cardiomyocytes and blood under psychoemotional stress. It was found that 40-day isolation and violation of diurnal cycle among the animals were accompanied by the intensification of lipid peroxidation process and marked with a reduced activity of antioxidant system enzymes, such as catalase and superoxide dismutase activity. The results suggested that psycho-emotional stress was accompanied by oxidative stress, causing a reduction in the intensity of energy metabolism in cardiomyocytes, which was further strengthened by the fact that the activity of the enzymes involved in ATP synthesis in mitochondria was reduced. Based on the results, we proposed that psychological stress is one of the factors contributing to the development of various cardiac diseases.

show abstract

Determination of inorganic phosphate by the molybdovanadate method in the presence of ATP and some interfering organic bases

Cited by 44 publications

References 7 publications

Heat Stress Reduces Intestinal Barrier Integrity and Favors Intestinal Glucose Transport in Growing Pigs

Heat Stress Reduces Intestinal Barrier Integrity and Favors Intestinal Glucose Transport in Growing Pigs

Improvement of Bone Strength and Dermal Thickness Due to Dietary Edible Bird’s Nest Extract in Ovariectomized Rats

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Contact Info

Product

Resources

About